The statistical verification of the minimum experimental datasets for the isolation of plant secondary metabolites related transcription factors using RNA-Seq analysis technique.
Project/Area Number |
26350967
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Biomolecular chemistry
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Research Institution | Kazusa DNA Research Institute |
Principal Investigator |
Suzuki Hideyuki 公益財団法人かずさDNA研究所, バイオ研究開発部, グループ長 (80276162)
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Co-Investigator(Kenkyū-buntansha) |
松田 一彦 近畿大学, 農学部, 教授 (00199796)
尾形 善之 大阪府立大学, 生命環境科学研究科, 准教授 (90446542)
水谷 正治 神戸大学, 農学研究科, 准教授 (60303898)
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Co-Investigator(Renkei-kenkyūsha) |
Mannen Kazuto (公財)かずさDNA研究所, バイオ研究開発部, 特任研究員 (60744216)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
Keywords | RNAーSeq解析 / ネットワーク解析 / 相関解析 / 生合成酵素遺伝子 / 除虫菊 / ピレスリン / アントシアニン / 転写因子 / RNA-Seq解析 / 相関ネットワーク解析 / 生合成関連酵素 |
Outline of Final Research Achievements |
We established standalone ConfeitoGUIplus software for detecting local communities from a correlation network involving size sensitivity. The developed software accepts any kind of a multivariate dataset and a correlation matrix. Using the public Arabidopsis DNA array resource data (more than 9 thousands), we have shown an application of our developed software to isolate gene co-expression network module in Arabidopsis flavonol biosynthesis. We performed RNA-Seq analysis in Tanacetum cinerariifolium for gene discovery to isolate biosynthetic emzyme involved in natural pyrethrins biosynthesis. We have investigated the effects of the blend of the volatile organic compounds (VOCs) on gene expressions of seven biosynthetic enzymes.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.2017
Author(s)
Tamura, K., Seki, H., Suzuki, H., Kojoma, M., Saito, K. and Muranaka, T.: CYP716A179 functions as a triterpene C-28 oxidase in tissue-cultured stolons of Glycyrrhiza uralensis.
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Journal Title
Plant Cell Rep.
Volume: 36
Issue: 3
Pages: 437-445
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Seasonal induction of alternative principal pathway for rose flower scent.2016
Author(s)
Hirata, H., Ohnishi, T., Tomida, K., Ishida, H., Kanda, M., Sakai, M., Yoshimura, J., Suzuki, H., Ishikawa, T., Dohra, H., Watanabe, N
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Journal Title
Sci. Rep.
Volume: 6
Issue: 1
Pages: 1-9
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Tangled evolutionary processes with commonality and diversity in plastidial glycolipid synthesis in photosynthetic organisms2016
Author(s)
Hori, K., Nobusawa, T., Watanabe, T., Madoka, Y., Suzuki, H., Shibata, D., Shimojima, M., and Ohta, H.
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Journal Title
Biochim. Biophys. Acta, Molecular and Cell Biology of Lipids
Volume: in press
Issue: 9
Pages: 1294-1308
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Benzenoid biosynthesis in the flowers of Eriobotrya japonica: molecular cloning and functional characterization of p-methoxybenzoic acid carboxyl methyltransferase.2016
Author(s)
Koeduka, T., Kajiyama, M., Suzuki, H., Furuta, T., Tsuge, T. and Matsui, K.
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Journal Title
Planta
Volume: 244
Issue: 3
Pages: 725-736
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Characterization of an O-methyltransferase specific to guaiacol-type benzenoids from the flowers of loquat (Eriobotrya japonica)2016
Author(s)
Koeduka, T., Kajiyama, M., Furuta, T., Suzuki, H., Tsuge, T. and Matsui, K.
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Journal Title
Journal of Bioscience and Bioengineering
Volume: 122
Issue: 6
Pages: 679-684
DOI
NAID
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Comparative Characterization of the Leaf Tissue of Physalis alkekengi and Physalis peruviana Using RNA-seq and Metabolite Profiling2016
Author(s)
Atsushi Fukushima, Michimi Nakamura, Hideyuki Suzuki, Mami Yamazaki, Eva Knoch, Tetsuya Mori, Naoyuki Umemoto, Masaki Morita, Go Hirai, Mikiko Sodeoka, and Kazuki Saito
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Journal Title
Frontiers in Plant Science
Volume: 7
Pages: 1883-1883
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Targeted Integration of RNA-Seq and Metabolite Data to Elucidate Curcuminoid Biosynthesis in Four Curcuma Species2015
Author(s)
Li D, Ono N, Sato T, Sugiura T, Altaf-Ul-Amin M, Ohta D, Suzuki H, Arita M, Tanaka K, Ma Z, Kanaya S
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Journal Title
Plant Cell Physiol
Volume: 2015
Issue: 5
Pages: 843-51
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Transcriptome Analysis of Nine Tissues to Discover Genes Involved in the Biosynthesis of Active Ingredients in <i>Sophora flavescens</i>2015
Author(s)
Han R, Takahashi H, Nakamura M, Bunsupa S, Yoshimoto N, Yamamoto H, Suzuki H, Shibata D, Yamazaki M, Saito K.
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Journal Title
Biological and Pharmaceutical Bulletin
Volume: 38
Issue: 6
Pages: 876-883
DOI
NAID
ISSN
0918-6158, 1347-5215
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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