| Project/Area Number |
26390054
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Crystal engineering
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| Research Institution | The University of Tokushima |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
藤原 貴久 徳島大学, 大学院理工学研究部, 教務補佐員 (10648695)
佐藤 正英 金沢大学, 総合メディア基盤センター, 教授 (20306533)
佐崎 元 北海道大学, 低温科学研究所, 教授 (60261509)
勝野 弘康 立命館大学, 理工学部, 助教 (70377927)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脱塩 / 遠心濃縮 / 結晶構造解析 / 液液相分離 / 融液様成長 / リゾチーム / グリセロール / グルコースイソメラーゼ / 酵素活性 / 分子ステップ |
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| Outline of Final Research Achievements |
Salt-free protein crystallization method with centrifugal concentration apparatus has been developed. With this method, nucleation of hen egg-white lysozyme (HEWL) crystals occurs in hyper-concentrated liquid phase obtained by liquid-liquid phase separation during centrifugal concentration processes. We could successfully observed molecular steps on the obtained crystal surface. Three-dimensional structure of lysozyme molecule obtained in this method is significantly different from that obtained by normal salting-out method.
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