Improvemtnt of reactive pyrolysis GC/MS system for highly sensitive detection of drug-resistant bacteria
| Project/Area Number |
26410164
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Chubu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
川村 久美子 名古屋大学, 医学系研究科(保健), 准教授 (30335054)
堤内 要 中部大学, 応用生物学部, 教授 (50329851)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 消毒薬耐性菌 / 薬剤耐性菌 / バイオ分析 / 細菌の精密化学計測 |
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| Outline of Final Research Achievements |
A reactive pyrolysis GC/MS system combined with magnetic bead probe was newly developed. This technique realized 1) selective capture of a certain species of bacteria, and 2 ) fatty acid analysis of the species. Moreover, this method was applied to the analysis of fatty acid components in mutant-type E. coli with resistance to 5-chloro-2-(2,4-dichlorophenoxyl)phenol (triclosan). Based on the pattern of the obtained chromatograms, the mutant colonies were clearly discriminated from the wild type ones reflecting their resistance to triclosan.
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Report
(4 results)
Research Products
(22 results)
