Functional analyses of TCTP in neural stem/progenitor cells

• Project/Area Number: 26430074
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurochemistry/Neuropharmacology
• Research Institution: Keio University
• Principal Investigator: Ohta Shigeki 慶應義塾大学, 医学部(信濃町), 講師 (20365406)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 神経幹細胞 / グリオーマ幹細胞 / TCTP1 / ＴＣＴＰ１ / グリオーマ / エピジェネティクス / 再生医療 / ＴＣＴＰ1

Outline of Final Research Achievements

MIF (Macrophage migration inhibitory factor) was identified as a functional molecule, which supports the proliferation of murine neural stem/progenitor cells (NSPCs). We also identified a new factor TCTP1 (Tumor Protein Translationally-Controlled 1), contributing to the cell proliferation of mouse NSPCs. TCTP1 gene expression was regulated by MIF and TCTP1 gene silencing defected neurogenesis in human ES cell-derived neural stem cells (hES-NSCs). We also found that MIF-TCTP1-miR338-SMO signaling cascade regulated the cell proliferation in hES-NSCs. In addition, TCTP1 supported the glioma-initiating cell proliferation regulated by MIF.

Research Products

• [Journal Article] CHD7 promotes proliferation of neural stem cells mediated by MIF (2016)
  • Author(s): Ohta S, Yaguchi T, Okuno H, Chneiweiss H, Kawakami Y, Okano H
  • Journal Title: Molecular Brain Volume: 13 Issue: 1 Pages: 96-107
  • DOI: 10.1186/s13041-016-0275-6
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Functional analyses of TPT1 in neural stem/progenitor cells and glioma initiating cells (2017)
  • Author(s): Ohta S, Okano H, Kawakami Y
  • Organizer: 第76回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（横浜市西区）
  • Year and Date: 2017-09-28
• [Presentation] Functional analyses of TPT1 in neural stem/progenitor cells and glioma initiating cells (2017)
  • Author(s): Ohta S, Kawakami Y, Okano H
  • Organizer: ISN 2017 Meeting
  • Place of Presentation: パリ（フランス）
  • Year and Date: 2017-08-20
  • Int'l Joint Research
• [Presentation] TPT1 regulates the proliferation and/or survival of Neural stem/progenitor cells (2016)
  • Author(s): Ohta S, Kawakami Y, Okano H
  • Organizer: 第59回日本神経化学大会
  • Place of Presentation: 福岡国際会議場（福岡市博多区）
  • Year and Date: 2016-09-08
• [Presentation] TPT1 regulates the proliferation and/or survival of Neural stem/progenitor cells (2016)
  • Author(s): 大多茂樹、河上裕、岡野栄之
  • Organizer: 第59回日本神経化学会大会
  • Place of Presentation: 福岡国際会議場（福岡県福岡市）
  • Year and Date: 2016-09-08