Functional analysis of amino acid transporter LAT1 in tumor endothelial cells
Project/Area Number |
26430121
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Tumor biology
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Research Institution | Kyorin University |
Principal Investigator |
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Co-Investigator(Renkei-kenkyūsha) |
WADA Youichiro 東京大学, アイソトープ総合センター, 教授 (10322033)
SAKURAI Hiroyuki 杏林大学, 医学部, 教授 (00508294)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | がん微小環境 / アミノ酸トランスポーター / 腫瘍血管新生 / LAT1 / 血管内皮細胞 / LAT1 |
Outline of Final Research Achievements |
To elucidate the pathological roles of system L amino acid transporter LAT1 in tumor microenvironment, we examined in vitro endothelial function and in vivo tumor angiogenesis using LAT1 selective inhibitor, JPH203. JPH203 elicited amino acid deprivation stress and impaired endothelial cell growth, migration and tube formation in human umbilical vein endothelial cells. In mouse B16F10 melanoma xenograft model, JPH203 significantly inhibited tumor growth by attenuating angiogenesis. Taken together, these findings have provided the new insight in LAT1 function in tumor progression and showed the possibility of JPH203 as an angiogenesis inhibitor.
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Wolf-Hirschhorn syndrome candidate 1-like 1 epigenetically regulates nephrin gene expression.2017
Author(s)
Ito Y, Katayama K, Nishibori Y, Akimoto Y, Kudo A, Kurayama R, Hada I, Takahashi S, Kimura T, Fukutomi T, Katada T, Suehiro J, Beltcheva O, Tryggvason K, Yan K.
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Journal Title
Am J Physiol Renal Physiol.
Volume: -
Issue: 6
Pages: F1184-F1199
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Dynamically and epigenetically coordinated GATA/ETS/SOX transcription is indispensable for endothelial cell differentiation2017
Author(s)
Kanki Y, Nakaki R, Shimamura T, Matsunaga T, Yamamizu K, Katayama S, Suehiro JI, Osawa T, Aburatani H, Kodama T, Wada Y, Yamashita JK, Minami T.
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Journal Title
Nucleic Acids Research
Volume: 印刷中
Issue: 8
Pages: 4344-4358
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
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Journal Title
Genome Biology
Volume: 15
Issue: 4
Pages: R63-R63
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Direct Evidence for Pitavastatin Induced Chromatin Structure Change in the KLF4 Gene in Endothelial Cells.2014
Author(s)
Maejima T, Inoue T, Kanki Y, Kohro T, Li G, Ohta Y, Kimura H, Kobayashi M, Taguchi A, Tsutsumi S, Iwanari H, Yamamoto S, Aruga H, Dong S, Stevens JF, Poh HM, Yamamoto K, Kawamura T, Mimura I,
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Journal Title
PLoS One.
Volume: 9
Issue: 5
Pages: e96005-e96005
DOI
Related Report
Peer Reviewed / Open Access
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