Elucidation of dedifferentiation mechanism by transcriptome analysis
| Project/Area Number |
26430185
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Genome biology
|
|---|
| Research Institution | Hiroshima University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
穂積 俊矢 広島大学, 理学研究科, 助教 (10597222)
武藤 彰彦 広島大学, 理学研究科, 助教 (70589366)
|
|---|
| Co-Investigator(Renkei-kenkyūsha) |
Machida Masayuki 独立行政法人産業技術総合研究所, 生物プロセス研究部門, 総括研究主幹 (30358006)
Kokubo Hiroki 広島大学, 大学院医歯薬保健学研究院, 講師 (10270480)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
|---|
| Keywords | ゼブラフィッシュ / 再生 / トランスクリプトーム解析 / トランスクリプトーム |
|---|
| Outline of Final Research Achievements |
Some vertebrates such as teleosts and amphibians can regenerate their lost appendages, whereas mammalians have limited regeneration ability. In animals that have regenerative ability, the restart of cell proliferation occurs during regeneration. Although this phenomenon is well known as “dedifferentiation”, the mechanism of this dedifferentiation remains elusive. To elucidate this dedifferentiation mechanism, I tried to screen genes that are expressed in the dedifferentiated proliferative cells during zebrafish fin regeneration by using next-generation sequencing. I identified the 132 genes that are up-regulated their expression more than twice after fin amputation compared with those before fin amputation. I am planning to analyze the function of these genes in dedifferentiation processes.
|
Report
(4 results)
Research Products
(27 results)
-
-
[Journal Article] Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle.2017
Author(s)
Fujii, M., Sakaguchi, A., Kamata, R., Nagao, M., Kikuchi, Y., Evans, S.M., Yoshizumi M., Shimono, A., Saga, Y., and Kokubo, H.
-
Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 14664-14664
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
-
-
-
-
-
-
[Journal Article] Nipbl and mediator cooperatively regulate gene expression to control limb development.2014
Author(s)
Muto, A., Ikeda, S., Lopez-Burks, M.E., Kikuchi, Y., Calof, A.L., Lander, A.D., Schilling, T.F.
-
Journal Title
PLOS Genetics
Volume: 10
Issue: 9
Pages: e1004671-e1004671
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] No evidence for AID/MBD4-coupled DNA demethylation in zebrafish embryos.2014
Author(s)
Shimoda, N., Hirose, K., Kaneto, R., Izawa, T., Yokoi, H., Hashimoto, N. and Kikuchi, Y
-
Journal Title
PLoS ONE
Volume: 9
Issue: 12
Pages: e114816-e114816
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
