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Elucidation of dedifferentiation mechanism by transcriptome analysis

Research Project

Project/Area Number 26430185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Genome biology
Research InstitutionHiroshima University

Principal Investigator

Kikuchi Yutaka  広島大学, 理学研究科, 教授 (20286438)

Co-Investigator(Kenkyū-buntansha) 穂積 俊矢  広島大学, 理学研究科, 助教 (10597222)
武藤 彰彦  広島大学, 理学研究科, 助教 (70589366)
Co-Investigator(Renkei-kenkyūsha) Machida Masayuki  独立行政法人産業技術総合研究所, 生物プロセス研究部門, 総括研究主幹 (30358006)
Kokubo Hiroki  広島大学, 大学院医歯薬保健学研究院, 講師 (10270480)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsゼブラフィッシュ / 再生 / トランスクリプトーム解析 / トランスクリプトーム
Outline of Final Research Achievements

Some vertebrates such as teleosts and amphibians can regenerate their lost appendages, whereas mammalians have limited regeneration ability. In animals that have regenerative ability, the restart of cell proliferation occurs during regeneration. Although this phenomenon is well known as “dedifferentiation”, the mechanism of this dedifferentiation remains elusive. To elucidate this dedifferentiation mechanism, I tried to screen genes that are expressed in the dedifferentiated proliferative cells during zebrafish fin regeneration by using next-generation sequencing. I identified the 132 genes that are up-regulated their expression more than twice after fin amputation compared with those before fin amputation. I am planning to analyze the function of these genes in dedifferentiation processes.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (27 results)

All 2017 2016 2015 2014

All Journal Article (10 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 10 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (17 results) (of which Invited: 4 results)

  • [Journal Article] A limited role for Planar Cell Polarity during early endoderm morphogenesis.2017

    • Author(s)
      Miles, L.B., Mizoguchi, T., Kikuchi, Y., and Verkade, H.
    • Journal Title

      Biology Open

      Volume: 印刷中

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Sfrp5 identifies murine cardiac progenitors for all myocardial structures except for the right ventricle.2017

    • Author(s)
      Fujii, M., Sakaguchi, A., Kamata, R., Nagao, M., Kikuchi, Y., Evans, S.M., Yoshizumi M., Shimono, A., Saga, Y., and Kokubo, H.
    • Journal Title

      Nature Communications

      Volume: 8 Issue: 1 Pages: 14664-14664

    • DOI

      10.1038/ncomms14664

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Magnetically tunable control of light reflection in an unusual optical protein of squid2017

    • Author(s)
      M Iwasaka, K Tagawa, Y Kikuchi
    • Journal Title

      AIP Advances

      Volume: 7 (5) Issue: 5 Pages: 056722-056722

    • DOI

      10.1063/1.4976938

    • NAID

      120006549334

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Histone H3 lysine 27 trimethylation leads to loss of mesendodermal competence during gastrulation in zebrafish ectodermal cells.2017

    • Author(s)
      Shiomi T., Muto A., Hozumi S., Kimura H., and Kikuchi, Y.
    • Journal Title

      Zoological Science

      Volume: 34 Issue: 1 Pages: 64-71

    • DOI

      10.2108/zs160032

    • NAID

      210000176852

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish2016

    • Author(s)
      Y. Nakahara, A. Muto, R. Hirabayashi, T. Sakuma, T. Yamamoto, S. Kume, and Y. Kikuchi
    • Journal Title

      Genes to Cells

      Volume: 21 Issue: 5 Pages: 492-504

    • DOI

      10.1111/gtc.12358

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Journal Article] Decrease in cytosine methylation at CpG island shores and increase in DNA fragmentation during zebrafish aging2014

    • Author(s)
      Shimoda, N., T. Izawa, A. Yoshizawa, H. Yokoi, Y. Kikuchi and N. Hashimoto
    • Journal Title

      Age

      Volume: 36 Issue: 1 Pages: 103-115

    • DOI

      10.1007/s11357-013-9548-5

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression patterns of dnmt3aa, dnmt3ab, and dnmt4 during development and fin regeneration in zebrafish2014

    • Author(s)
      K Takayama, N Shimoda, S Takanaga, S Hozumi, Y Kikuchi.
    • Journal Title

      Gene Expression Patterns

      Volume: 14(2) Issue: 2 Pages: 105-110

    • DOI

      10.1016/j.gep.2014.01.005

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nipbl and mediator cooperatively regulate gene expression to control limb development.2014

    • Author(s)
      Muto, A., Ikeda, S., Lopez-Burks, M.E., Kikuchi, Y., Calof, A.L., Lander, A.D., Schilling, T.F.
    • Journal Title

      PLOS Genetics

      Volume: 10 Issue: 9 Pages: e1004671-e1004671

    • DOI

      10.1371/journal.pgen.1004671

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mechanistic target of rapamycin complex 1 signaling regulates cell proliferation, cell survival, and differentiation in regenerating zebrafish fins.2014

    • Author(s)
      Hirose K, Shiomi T, Hozumi S, Kikuchi Y.
    • Journal Title

      BMC Dev Biol.

      Volume: 14 Issue: 1 Pages: 42-42

    • DOI

      10.1186/s12861-014-0042-9

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] No evidence for AID/MBD4-coupled DNA demethylation in zebrafish embryos.2014

    • Author(s)
      Shimoda, N., Hirose, K., Kaneto, R., Izawa, T., Yokoi, H., Hashimoto, N. and Kikuchi, Y
    • Journal Title

      PLoS ONE

      Volume: 9 Issue: 12 Pages: e114816-e114816

    • DOI

      10.1371/journal.pone.0114816

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ゼブラフィッシュ初期発生における核内F-アクチンの機能解析2016

    • Author(s)
      青木 駿, 穂積 俊矢, 菊池 裕
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] ゼブラフィッシュの尾ビレ損傷時における表皮特異的な異常細胞増殖の解析2016

    • Author(s)
      高山 和也, Jingxin Wang, 小松原 康史, 菊池 裕
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 染色体制御因子NIPBLによる細胞骨格を介した脂肪細胞分化の新規制御機構2016

    • Author(s)
      武藤 彰彦, 片山 大也, 菊池 裕
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] 核の移動制御を介したNodalシグナルによる内胚葉分化機構の解明2016

    • Author(s)
      穂積俊矢,青木駿,菊池 裕
    • Organizer
      日本遺伝学会
    • Place of Presentation
      三島市
    • Year and Date
      2016-09-07
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] ゼブラフィッシュ尾ビレ再生におけるmTORC1の機能解析2015

    • Author(s)
      廣瀬健太郎,高山和也,塩見太志,穂積俊矢,菊池 裕
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-04
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 脊椎動物の中内胚葉分離における時空間的制御機構の解析2015

    • Author(s)
      穂積俊矢,青木 駿,菊池 裕
    • Organizer
      第38回日本分子生物学会年会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-03
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 脊椎動物の中内胚葉分離における時空 間的制御機構の解析2015

    • Author(s)
      穂積俊矢,青木 駿,菊池 裕
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] 三胚葉分化過程における細胞運命決定 の可塑性制御機構の解明2015

    • Author(s)
      塩見太志、武藤彰彦、木村 宏、 菊池 裕
    • Organizer
      日本遺伝学会第87回大会
    • Place of Presentation
      東北大学川内北キャンパス
    • Year and Date
      2015-09-26
    • Related Report
      2015 Research-status Report
  • [Presentation] ゼブラフィッシュの尾ビレ再 生における位置特異的再 生機構の解析2015

    • Author(s)
      高山 和也,菊池 裕
    • Organizer
      日本動物学会第86 回大会
    • Place of Presentation
      朱鷺メッセ
    • Year and Date
      2015-09-17
    • Related Report
      2015 Research-status Report
  • [Presentation] 四肢発生過程における染色体高次構造形成を介した遺伝子発現調節機構2014

    • Author(s)
      武藤彰彦、池田晋悟、Lopez-Burks Martha, 菊池 裕,Calof Anne, Lander Arthur, Schilling Thomas
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Mechanistic target of rapamycin complex1(mTORC1)はゼブラフィッシュ尾ビレ再生に必要である2014

    • Author(s)
      廣瀬健太朗、塩見太志、穂積俊矢、菊池 裕
    • Organizer
      日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 遺伝子発現制御解析による細胞可塑性コントロール機構の解明2014

    • Author(s)
      穂積 俊矢、宮本 良祐、粂 昭苑、菊池 裕
    • Organizer
      日本生化学会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2014-10-15 – 2014-10-18
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] AIDとMBD4による脱メチル化はゼブラフィッシュ胚で起こるのか?2014

    • Author(s)
      下田修義、廣瀬健太朗、井澤俊明、横井勇人、橋本有弘、菊池 裕
    • Organizer
      日本遺伝学会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] ゼブラフィッシュの発生・再生過程におけるマウスdnmt3相同遺伝子の発現・機能解析2014

    • Author(s)
      髙山和也、下田修義、高永俊祐、穂積俊矢、菊池 裕
    • Organizer
      日本遺伝学会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] 四肢発生過程における染色体高次構造形成を介した遺伝子発現調節機構2014

    • Author(s)
      武藤彰彦、池田晋悟、Lopez-Burks Martha, 菊池 裕,Calof Anne, Lander Arthur, Schilling Thomas
    • Organizer
      日本遺伝学会
    • Place of Presentation
      長浜バイオ大学
    • Year and Date
      2014-09-17 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] ターゲットオブラパマイシン複合体1(TORC1)は、ゼブラフィッシュ尾ビレ再生を制御する2014

    • Author(s)
      廣瀬健太朗、塩見太志、穂積俊矢、菊池 裕
    • Organizer
      日本動物学会
    • Place of Presentation
      東北大学
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 再生過程におけるマウスdnmt3相同遺伝子の発現・機能解析2014

    • Author(s)
      髙山和也、下田修義、高永俊祐、穂積俊矢、菊池 裕
    • Organizer
      日本動物学会中国四国支部
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2014-05-10 – 2014-05-11
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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