Analysis for structure and DNA/RNA binding activity of the bacterial cytoskeletal protein RodZ
| Project/Area Number |
26440014
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
Jiro Mitobe 国立感染症研究所, 細菌第一部, 主任研究官 (40333364)
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| Co-Investigator(Kenkyū-buntansha) |
柳原 格 地方独立行政法人大阪府立病院機構大阪母子医療センター(研究所), 免疫部門, 部長 (60314415)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | バクテリア細胞骨格 / RNA結合蛋白 / 転写後調節 / 蛋白局在 / 細胞骨格蛋白 / 局在 / 赤痢菌 / ワクチン / 多量体形成能 / ジスルフィド結合 / 多量体形成 / RNA結合 / DNA結合 / DNA結合蛋白 |
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| Outline of Final Research Achievements |
Bacterial cytoskeletal protein RodZ, which is involved in formation of rod shape of bacilli, is also involved in post-transcriptional regulation of invE(virB) gene in the type 3 secretion system of Shigella through the RNA binding activity. In this study, we found that the RNA binding activity was depended on hexamer formation and clustering ability of multiple hexamers. Such cluster was consistently detected by immuno-fluorescent microscopy with super resolution confocal setting. In an attempt to isolate genes under regulation by RodZ, we found that expression of rpoS and rpoH genes encoding sigma subunits of RNA polymerase, which have been well-characterized post-transcriptional regulation, were increased by rodZ mutation. Analysis of DNA and RNA binding activities indicated that sequence specificity was not identified. In addition, the binding activity was sensitive to the salt concentration.
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Report
(5 results)
Research Products
(16 results)
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[Journal Article] Intracellular fate of Ureaplasma parvum entrapped by host cellular autophagy2017
Author(s)
Nishiumi F, Ogawa M, Nakura Y, Hamada Y, Nakayama M, Mitobe J, Hiraide A, Sakai N, Takeuchi M, Yoshimori T, Yanagihara I*
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Journal Title
MicrobiologyOpen
Volume: -
Issue: 3
Pages: 1-15
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Epidemiological analysis of a large enterohaemorrhagic Escherichia coli O111 outbreak in Japan associated with haemolytic uraemic syndrome and acute encephalopathy.2015
Author(s)
Yahata Y, Misaki T, Ishida Y, Nagira M, Watahiki M, Isobe J, Terajima J, Iyoda S, Mitobe J, Ohnishi M, Sata T, Taniguchi K, Tada Y, Okabe N; E. coli O111 Outbreak Investigation Team.
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Journal Title
Epidemiol Infect.
Volume: 143
Issue: 13
Pages: 2721-2732
DOI
Related Report
Peer Reviewed
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[Journal Article] Epidemiological analysis of a large enterohaemorrhagic Escherichia coli O111 outbreak in Japan associated with haemolytic uraemic syndrome and acute encephalopathy.2015
Author(s)
Yahata Y, Misaki T, Ishida Y, Nagira M, Watahiki M, Isobe J, Terajima J, Iyoda S, Mitobe J, Ohnishi M, Sata T, Taniguchi K, Tada Y, Okabe N
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Journal Title
Epidemiol Infect PMID: 25600435
Volume: 20
Pages: 1-12
Related Report
Peer Reviewed
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[Journal Article] Characterization of enterohemorrhagic Escherichia coli O111 and O157 strains isolated from outbreak patients in Japan.2014
Author(s)
Watahiki M, Isobe J, Kimata K, Shima T, Kanatani J, Shimizu M, Nagata A, Kawakami K, Yamada M, Izumiya H, Iyoda S, Morita-Ishihara T, Mitobe J, Terajima J, Ohnishi M, Sata T.
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Journal Title
J Clin Microbiol.
Volume: 52(8)
Issue: 8
Pages: 2757-63
DOI
Related Report
Peer Reviewed
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