Molecular mechanism of interaction between transcriptional factors Sp1 and TAF4.
| Project/Area Number |
26440025
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
HIBINO Emi 京都大学, 大学院薬学研究科
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 転写因子 / 分子間相互作用 / 天然変性蛋白質 / 核磁気共鳴分光法 / グルタミンリッチドメイン / ＮＭＲ |
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| Outline of Final Research Achievements |
The expression of eukaryotic genes is precisely controlled by specific interactions between general transcription initiation factors and gene-specific transcriptional activators. The general transcription factor TFIID plays an essential role in mediating transcriptional activation. On the other hand, biochemical approaches have shown that the promoter-specific transcriptional activator Sp1 interacts with one of the components of TFIID, the TBP-associated factor TAF4.
We herein report the structural details of the glutamine-rich domains (Q-domains) of Sp1 and TAF4. We found that the two Q-domains of Sp1 and four Q-domains of TAF4 were disordered under physiological conditions. We also quantitatively analyzed the interaction between the Q-domains of Sp1 and TAF4 by NMR and surface plasmon resonance, and detected a significant association between them.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] The unexpected role of polyubiquitin chains in the formation of fibrillar aggregates.2015
Author(s)
Morimoto D,Walinda E, Fukada H, Sou YS, Kageyama S, Hoshino M, Fujii T, Tsuchiya H, Saeki Y, Arita K, Ariyoshi M, Tochio H, Iwai K, Namba K, Komatsu M, Tanaka K, Shirakawa M.
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Journal Title
Nat Commun.
Volume: 6
Issue: 1
Pages: 6116-6116
DOI
NAID
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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