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Phosphorylation-dependent regulatory mechanism of calmoudlin-kinase cascade

Research Project

Project/Area Number 26440056
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Functional biochemistry
Research InstitutionOkayama University

Principal Investigator

Tokumitsu Hiroshi  岡山大学, 自然科学研究科, 教授 (20237077)

Co-Investigator(Kenkyū-buntansha) 曲 正樹  岡山大学, 自然科学研究科, 助教 (50359882)
Co-Investigator(Renkei-kenkyūsha) HATANO Naoya  神戸大学, 大学院医学(系)研究科, 特命助教 (10332280)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsCaMKK / AMPK / STO-609 / CaMK cascade / Protein Kinase / Signal Transduction / リン酸化反応 / AMPキナーゼ / CaMキナーゼI V / 細胞内カルシウム / シグナル伝達 / 特異性 / CaMK Cascade
Outline of Final Research Achievements

Intracellular Ca2+ plays an important role in the cellular signal transduction system as a second messenger. Calmodulin (caM) is one of Ca2+-binding proteins, which activates multifunctional CaM-kinases. In this research, we examined the molecular mechanism of substrate recognition for Ca2+/calmodulin-dependent protein kinase kinase beta based on the structure-function analysis.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (19 results)

All 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Acknowledgement Compliant: 2 results,  Open Access: 1 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (2 results)

  • [Int'l Joint Research] University of Southern California/Keck School of Medicine(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Differential AMP-activated Protein Kinase (AMPK) Recognition Mechanism of Ca2+/Calmodulin-dependent Protein Kinase Kinase Isoforms.2016

    • Author(s)
      Yuya Fujiwara, Yoshinori Kawaguchi, Tomohito Fujimoto, Naoki Kanayama, Masaki Magari, Hiroshi Tokumitsu
    • Journal Title

      The Journal of biological chemistry

      Volume: 291 Issue: 26 Pages: 13802-13808

    • DOI

      10.1074/jbc.m116.727867

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of striated muscle activator of Rho signaling (STARS) as a novel calmodulin target by a newly developed genome-wide screen.2016

    • Author(s)
      Yusui Furuya, Miwako Denda, Kyohei Sakane, Tomoko Ogusu, Sumio Takahashi, Masaki Magari, Naoki Kanayama, Ryo Morishita, Hiroshi Tokumitsu
    • Journal Title

      Cell calcium

      Volume: 60 Issue: 1 Pages: 32-40

    • DOI

      10.1016/j.ceca.2016.04.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative Stress Impairs the Stimulatory Effect of S100 Proteins on Protein Phosphatase 5 Activity2016

    • Author(s)
      Yamaguchi F, Tsuchiya M, Shimamoto S, Fujimoto T, Tokumitsu H, Tokuda M, and Kobayashi R
    • Journal Title

      The Tohoku Journal of Experimental Medicine

      Volume: 240 Issue: 1 Pages: 67-78

    • DOI

      10.1620/tjem.240.67

    • NAID

      130006776928

    • ISSN
      0040-8727, 1349-3329
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Distinct Roles of CaMKK Isoforms using STO-609-Resistant Mutants in Living Cells.2015

    • Author(s)
      Fujiwara Y, Hiraoka Y, Fujimoto T, Kanayama N, Magari M, Tokumitsu H.
    • Journal Title

      Biochemistry

      Volume: 54 Issue: 25 Pages: 3969-3977

    • DOI

      10.1021/acs.biochem.5b00149

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] NUMB phosphorylation destabilizes p53 and promotes self-renewal of tumor-initiating cells by a NANOG-dependent mechanism in liver cancer.2015

    • Author(s)
      Siddique HR, Feldman DE, Chen CL, Punj V, Tokumitsu H, Machida K.
    • Journal Title

      Hepatology

      Volume: 62 Issue: 5 Pages: 1466-1479

    • DOI

      10.1002/hep.27987

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Compartmentalized AMPK Signaling Illuminated by Genetically Encoded Molecular Sensors and Actuators2015

    • Author(s)
      Miyamoto T, Rho E, Sample V, Akano H, Magari M, Ueno T, Gorshkov K, Chen M, Tokumitsu H, Zhang J, Inoue T
    • Journal Title

      Cell Reports

      Volume: 印刷中 Issue: 4 Pages: 657-670

    • DOI

      10.1016/j.celrep.2015.03.057

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Oxidized S100A4 inhibits the activation of protein phosphatase 5 through S100A1 in MKN‑45 gastric carcinoma cells2014

    • Author(s)
      Tsuchiya M, Yamaguchi F, Shimamoto S, Fujimoto T, Tokumitsu H, Tokuda M, Kobayashi R
    • Journal Title

      International Journal of Molecular Medicine

      Volume: 34 Issue: 6 Pages: 1713-1719

    • DOI

      10.3892/ijmm.2014.1947

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] Ca2+/Calmodulin-dependent Protein Kinase Kinaseシグナル伝達機構の茶道原理の解明(基質認識機構)2016

    • Author(s)
      藤原侑哉、川口義典、藤本智仁、金山直樹、曲正樹、徳光 浩
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
  • [Presentation] Calmodulin Signal Networkのインターラクトーム解析2015

    • Author(s)
      徳光 浩
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] CaMKKβによる選択的AMPK活性化の細胞生物学的および生化学的解析2015

    • Author(s)
      藤原 侑哉、川口 義典、藤本 智仁、曲 正樹、金山 直樹、徳光 浩
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] Ca2+/Calmodulin結合型転写因子の網羅的同定2015

    • Author(s)
      太尾田 泰成、大西 和貴、古谷 雄穂、傳田 美和子、金山 直樹、曲 正樹、森下 了、徳光  浩
    • Organizer
      第38回日本分子生物学会年会 第88回日本生化学会大会 合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2015-12-01
    • Related Report
      2015 Research-status Report
  • [Presentation] COMPREHENSIVE IDENTIFICATION OF HUMAN CALMODULIN-TARGETS2015

    • Author(s)
      Furuya Y, Denda M, Kanayama N, Magari M, Morishita R, Tokumitsu H
    • Organizer
      19th International Symposium on Calcium Binding Proteins and Calcium Function In Health and Disease
    • Place of Presentation
      ナッシュビル・アメリカ
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ANALYSIS OF DISTINCT ROLES OF CAMKK ISOFORMS USING STO-609- RESISTANT MUTANTS IN LIVING CELLS2015

    • Author(s)
      Fujiwara Y, Hiraoka Y, Fujimoto T, Kanayama N, Magari M, Tokumitsu H
    • Organizer
      19th International Symposium on Calcium Binding Proteins and Calcium Function In Health and Disease
    • Place of Presentation
      ナッシュビル・アメリカ
    • Year and Date
      2015-05-30
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] ヒトCalmodulin標的分子の網羅的同定2014

    • Author(s)
      古谷 雄穂, 西口 みゆ, 傳田美和子, 曲 正樹,金山 直樹,森下 了, 徳光 浩
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-18
    • Related Report
      2014 Research-status Report
  • [Presentation] STO-609抵抗性変異体を用いたCaMKKシグナル伝達の機能解析2014

    • Author(s)
      藤原 侑哉, 平岡 佑理, 曲 正樹,金山 直樹,徳光 浩
    • Organizer
      第87回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2014-10-17
    • Related Report
      2014 Research-status Report
  • [Presentation] カルモデュリン標的分子の網羅解析から見た カルシウムシグナル伝達の多様性2014

    • Author(s)
      徳光 浩
    • Organizer
      日本薬学会関東支部会
    • Place of Presentation
      東京
    • Year and Date
      2014-10-04
    • Related Report
      2014 Research-status Report
    • Invited
  • [Remarks] 岡山大学・大学院自然科学研究科 細胞機能設計学研究室ホームページ

    • URL

      http://www.okayama-u.ac.jp/user/saibou/index.html

    • Related Report
      2016 Annual Research Report
  • [Remarks] 岡山大学 研究者総覧

    • URL

      http://soran.cc.okayama-u.ac.jp/view?l=ja&u=a94ada4de7b2082d74506e4da22f6611

    • Related Report
      2015 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-01-27  

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