Analysis of Hedgehog signaling-mediated regulation of cell dynamics in the early embryo

• Project/Area Number: 26440130
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Osaka Medical College (2015-2017); JT Biohistory Research Hall (2014)
• Principal Investigator: Akiyama-Oda Yasuko 大阪医科大学, 医学部, 非常勤講師 (80426650)
• Research Collaborator: ODA Hiroki 株式会社生命誌研究館, 主任研究員 (50396222)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: ゲノム / 遺伝子発現 / 初期発生 / ヘッジホッグシグナル / ヘッジホッグ / RNAi / RNA-seq / RNA干渉 / 遺伝子 / シグナル伝達 / 発生・分化

Outline of Final Research Achievements

Hedgehog signaling has an important role in various developmental events. In the embryo of the spider Parasteatoda tepidariorum, Hedgehog signaling regulates early developmental events, such as anterior-posterior patterning, orientation of the dorsal-ventral axis, and morphogenesis. In this study, we performed genome-wide searches to identify target genes of Hedgehog signaling. We combined RNAi and RNA-seq techniques and identified many target genes that show region-specific expression in the early embryo. One of the identified genes, msh is found to be involved in segmentation and formation of the germ band.

Research Products

• [Journal Article] A quantitative study of the diversity of stripe-forming processes in an arthropod cell-based field undergoing axis formation and growth (2018)
  • Author(s): Natsuki Hemmi, Yasuko Akiyama-Oda, Koichi Fujimoto, Hiroki Oda
  • Journal Title: Developmental Biology Volume: 437 Issue: 2 Pages: 84-104
  • DOI: 10.1016/j.ydbio.2018.03.001
  • Peer Reviewed / Open Access
• [Journal Article] The house spider genome reveals an ancient whole-genome duplication during arachnid evolution (2017)
  • Author(s): Schwager, E. E., Sharma, P. P., Clarke, T., Leite, D. J., Wierschin, T., Pechmann, M., Akiyama-Oda, Y., Esposito, L., Bechsgaard, J.,(35 authors omitted), Bucher, G., Gibbs, R. A., Coddington, J., Oda, H., Stanke, M., Ayoub, N.A., Prpic, N. M., Flot, J. F., Posnien, N., Richards, S., McGregor, A.
  • Journal Title: BMC Biology Volume: 15 Issue: 1 Pages: 62-62
  • DOI: 10.1186/s12915-017-0399-x
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evolutionary origin of type IV classical cadherins in arthropods. (2017)
  • Author(s): Sasaki M, Akiyama-Oda Y and Oda H
  • Journal Title: BMC Evol. Biol. Volume: 17 Issue: 1 Pages: 142-142
  • DOI: 10.1186/s12862-017-0991-2
  • Peer Reviewed / Open Access
• [Journal Article] Multi-color FISH facilitates analysis of cell-type diversification and developmental gene regulation in the Parasteatoda spider embryo (2016)
  • Author(s): Yasuko Akiyama-Oda and Hiroki Oda
  • Journal Title: Development, Growth and Differentiation Volume: 58 Issue: 2 Pages: 215-224
  • DOI: 10.1111/dgd.12263
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] A genome-wide study of Hedgehog signaling identifies a transcription factor gene mediating gene expression dynamics in the early spider embryo. (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H
  • Organizer: 18th International Congress of Developmental Biology (Singapore)
  • Int'l Joint Research
• [Presentation] A Hedgehog signaling network regulates the initiation of segmental oscillations in the early spider embryo. (2017)
  • Author(s): Akiyama-Oda, Y., Iwasaki-Yokozawa, S., Oda, H.
  • Organizer: 50th Annual Meeting of JSDB (Tokyo)
• [Presentation] Genome-wide identification of Hedgehog signaling targets in axial patterning of the early embryo of the spider Parasteatoda tepidariorum (2016)
  • Author(s): Yasuko Akiyama-Oda, Sawa Iwasaki-Yokozawa, and Hiroki Oda
  • Organizer: EuroEvoDevo（第６回ヨーロッパ進化発生生物学会）
  • Place of Presentation: スウェーデン、ウプサラ
  • Year and Date: 2016-07-26
  • Int'l Joint Research
• [Presentation] Analysis of Hedgehog signaling in the early spider embryo using RNAi and RNA-seq techniques (2015)
  • Author(s): Yasuko Akiyama-Oda, Sawa Iwasaki, and Hiroki Oda
  • Organizer: 第48回日本発生生物学会
  • Place of Presentation: つくば市
  • Year and Date: 2015-06-02
• [Presentation] Genomic approach to studying hedgehog-dependent axis formation. (2014)
  • Author(s): Yasuko Akiyama-Oda
  • Organizer: Roth lab. seminar
  • Place of Presentation: Cologne, Germany
  • Year and Date: 2014-09-18
  • Invited
• [Presentation] Genome-wide identification of Hedgehog targets in the spider Parasteatoda tepidariorum. (2014)
  • Author(s): Yasuko Akiyama-Oda
  • Organizer: Second International SpiderWeb Meeting
  • Place of Presentation: Jena, Germany
  • Year and Date: 2014-09-15
• [Presentation] オオヒメグモRNAi胚のRNAseqから考える体軸形成機構の進化. (2014)
  • Author(s): 秋山-小田康子、小田広樹
  • Organizer: 日本進化学会第16回大会
  • Place of Presentation: 大阪府高槻市
  • Year and Date: 2014-08-24
• [Presentation] Genome-wide analysis of Hedgehog target genes using RNAi and RNA-seq in the spider Prasteatoda tepidariorum. (2014)
  • Author(s): Yasuko Akiyama-Oda, Hiroki Oda
  • Organizer: 第47回日本発生生物学会
  • Place of Presentation: 愛知県名古屋市
  • Year and Date: 2014-05-29
• [Remarks] BRH Data Resources
  • URL: http://www.brh2.jp/index.html
• [Remarks] 生命誌研究館ホームページ
  • URL: http://www.brh.co.jp/research/lab04/
• [Remarks] Pt_SpiderBase
  • URL: http://www.spider-brh.jp/pt_spiderbase/