| Project/Area Number |
26440140
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KINOSHITA Toshinori 名古屋大学, トランスフォーマティブ生命分子研究所, 教授 (50271101)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | オーキシン / 細胞膜プロトンポンプ / 伸長生長 / 細胞膜H+-ATPase / プロトンポンプ / リン酸化 / 胚軸伸長 / H+-ATPase / ABP1 |
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| Outline of Final Research Achievements |
The purpose of this project is to clarify the regulatory mechanism of plasma membrane proton pump (H+-ATPase) phosphorylation in response to phytohormone auxin during the elongation growth of plant tissues. It was suggested that Auxin-Binding Protein1 (ABP1), which was one of the auxin receptor candidates in this mechanism, has no direct involvement by the analysis using ABP1 knockout mutant, abp1-TD1 and abp1-C1. As results of chemical screening and mutant screening, several mutant candidates that markedly reduced the auxin-induced H+-ATPase phosphorylation could be isolated. We are going to examine the isolated mutants. In addition, we also investigated the effect of brassinosteroids on phosphorylation of H+-ATPase and found some of the crosstalk with auxin action.
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