Study of functional evolution of adrenal steroid receptor in evolutionary process

• Project/Area Number: 26440159
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Hokkaido University
• Principal Investigator: Katsu Yoshinao 北海道大学, 理学研究院, 教授 (00332180)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 副腎ステロイド / ステロイドホルモン / ホルモン受容体 / 分子進化 / 電解質コルチコイド受容体 / 古代魚 / プロゲステロン / 副腎ステロイド受容体 / 分子シミュレーション

Outline of Final Research Achievements

The adrenal steroid receptor binds to adrenal steroids such as glucocorticoids and mineralocorticoids secreted from the adrenal cortex and is involved in the mechanism of maintaining the homeostasis of animals and endocrine secretions. However, it is still unknown at which stage of biological evolution the mechanism of maintaining endocrine homeostasis by adrenal steroid receptors found in higher vertebrates such as humans. The purpose of this research project was to clarify the process of adrenal steroid receptor gene appearance and function acquisition in evolution process. As a result, ancient fishes, gar and sturgeon have the adrenal steroid receptor genes like humans, and the adrenal steroid receptors from ancient fishes have functionally equivalent to human receptors. These results can be expected to serve as important clues to elucidate the molecular evolution of the adrenal steroid receptor.

Research Products

• [Int'l Joint Research] カリフォルニア大学/セントクラウド州立大学(米国)
• [Int'l Joint Research] University of California, San Diego/Medical University of South Carolina(米国)
• [Journal Article] Characterization of evolutionary trend in squamate estrogen receptor sensitivity (2016)
  • Author(s): Yatsu R, Katsu Y, Kohno S, Mizutani T, Ogino Y, Ohta Y, Myburgh J, van Wyk JH, Guillette Jr LJ, Miyagawa S, Iguchi T.
  • Journal Title: Gen Comp Endocrinol Volume: 238 Pages: 88-95
  • DOI: 10.1016/j.ygcen.2016.04.005
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular cloning and characterization of the aryl hydrocarbon receptors and aryl hydrocarbon receptor nuclear translocators in the American alligator (2016)
  • Author(s): Oka K, Kohno S, Ohta Y, Guillette LJ Jr, Iguchi T, Katsu Y
  • Journal Title: Gen Comp Endocrinol Volume: 238 Pages: 13-22
  • DOI: 10.1016/j.ygcen.2016.05.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Evolution of corticosteroid specificity for human, chicken, alligator and frog glucocorticoid receptors. (2016)
  • Author(s): Katsu Y, Kohno S, Oka K, Baker ME
  • Journal Title: Steroids Volume: 113 Pages: 38-45
  • DOI: 10.1016/j.steroids.2016.06.005
  • NAID: 120006337743
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] A second estrogen receptor from Japanese lamprey (Lethenteron japonicum) does not have activities for estrogen binding and transcription. (2016)
  • Author(s): Katsu Y, Cziko PA, Chandsawangbhuwana C, Thornton JW, Sato R, Oka K, Takei Y, Baker ME, Iguchi T
  • Journal Title: Gen Comp Endocrinol Volume: 236 Pages: 105-114
  • DOI: 10.1016/j.ygcen.2016.07.014
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Corticosteroid and progesterone transactivation of mineralocorticoid receptors from Amur sturgeon and tropical gar. (2016)
  • Author(s): Sugimoto A, Oka K, Sato R, Adachi S, Baker ME, Katsu Y
  • Journal Title: Biochem J Volume: 473 Issue: 20 Pages: 3655-3665
  • DOI: 10.1042/bcj20160579
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Molecular cloning and characterization of anti-Mullerian hormone (AMH) from the Japanese wrinkled frog, Rana rugosa. (2015)
  • Author(s): Kodama M, Suda M, Sakamoto D, Iwasaki T, Matsuo Y, Uno Y, Matsuda Y, Nakamura Y, Maekawa S, Katsu Y, Nakamura M
  • Journal Title: Endocrinology Volume: 156 Issue: 5 Pages: 1914-1923
  • DOI: 10.1210/en.2013-2053
  • Peer Reviewed
• [Journal Article] Estrogen receptor 1 (ESR1; ERα), not ESR2 (ERβ), modulates estrogen-induced sex reversal in the American alligator, a species with temperature-dependent sex determination (2015)
  • Author(s): Kohno, S., Bernhard, M.C., Katsu, Y., Zhu, J., Byan, T.A., Doheny, B.M., Iguchi, T. and Guillette, L.J.Jr.
  • Journal Title: Endocrinology Volume: 印刷中 Issue: 5 Pages: 1887-1899
  • DOI: 10.1210/en.2014-1852
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and characterization of the androgen receptor from the American alligator, Alligator mississippiensis. (2015)
  • Author(s): Miyagawa S, Yatsu R, Kohno S, Doheny BM, Ogino Y, Ishibashi H, Katsu Y, Ohta Y, Guillette LJ Jr, Iguchi T
  • Journal Title: Endocrinology Volume: 156 Issue: 8 Pages: 2015-2037
  • DOI: 10.1210/en.2015-2037
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Understanding the molecular basisi for differences in responses of fish estrogen receptor subtypes to environmental estrogens (2015)
  • Author(s): Tohyama S, Miyagawa S, Lange A, Ogino Y, Mizutani T, Tatarazako N, Katsu Y, Ihara M, Tanaka H, Ishibashi H, Kobayashi T, Tyler CR, Iguchi T
  • Journal Title: Environ Sci Technol Volume: 49 Issue: 12 Pages: 7439-7447
  • DOI: 10.1021/acs.est.5b00704
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Allosteric role of the amino-terminal A/B domain on corticosteroid transactivation of gar and human glucocorticoid receptors. (2015)
  • Author(s): Oka K, Hoang A, Okada D, Iguchi T, Baker ME, Katsu Y
  • Journal Title: J Steroid Biochem Mol Biol Volume: 154 Pages: 112-119
  • DOI: 10.1016/j.jsbmb.2015.07.025
  • NAID: 120005853068
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] TRPV4 associates environmental temprerature and sex determination in the American alligator (2015)
  • Author(s): Yatsu R, Miyagawa S, Kohno S, Saito S, Ogino Y, Katsu Y, Ohta Y, Tominaga M, Guillette LJ, Iguchi T
  • Journal Title: Scientific Reports Volume: 5 Issue: 1 Pages: 18581-18581
  • DOI: 10.1038/srep18581
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Differing species responsiveness of estrogenic contaminants in fish is conferred by the ligand binding domain of the estrogen receptor (2014)
  • Author(s): Miyagawa, S., Lange, A., Hirakawa, I., Tohyama, S., Ogino, Y., Mizutani, T., Kagami, Y., Kusano, T., Ihara, M., Tanaka, H., Tatarazako, N., Ohta, Y., Katsu, Y., Tyler, C.R. and Iguchi, T.
  • Journal Title: Environ. Sci. Technol. Volume: 未定 Issue: 9 Pages: 5254-5263
  • DOI: 10.1021/es5002659
  • Peer Reviewed / Open Access
• [Journal Article] Possible involvement of insulin-like growth factor 2 mRNA-binding protein 3 in zebrafish oocyte maturation as a novel cyclin B1 mRNA-binding protein that represses the translation in immature oocytes (2014)
  • Author(s): Takahashi, K., Kotani, T., Katsu, Y., Yamashita, M.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 448 Issue: 1 Pages: 22-27
  • DOI: 10.1016/j.bbrc.2014.04.020
  • NAID: 120005457022
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] LH-induced Expression of Ptger4b, a Prostaglandin E2 Receptor Indispensable for Ovulation of the Medaka Oryzias latipes, is Regulated by a Genomic Mechanism Involving Nuclear Progestin Receptor. (2014)
  • Author(s): Hagiwara, A.
  • Journal Title: Ｂｉｏｌ． Ｒｅｐｒｏｄ． Volume: 90 Issue: 6 Pages: 1-14
  • DOI: 10.1095/biolreprod.113.115485
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] 特異的アゴニストに対するエストロゲン受容体の種特異性 (2016)
  • Author(s): 佐藤塁・勝義直
  • Organizer: 日本比較内分泌学会
  • Place of Presentation: 神奈川県相模原市（北里大学相模原キャンパス）
• [Presentation] Cloning and characterization of Holocephalan elephant shark, Callorhinchus milii, estrogen receptors. (2015)
  • Author(s): 勝義直
  • Organizer: NIBBコンファレンス
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県・岡崎市）
  • Year and Date: 2015-11-30
  • Int'l Joint Research / Invited
• [Presentation] 古代魚の糖質コルチコイド受容体のホルモン応答性 (2015)
  • Author(s): 勝義直
  • Organizer: 日本動物学会
  • Place of Presentation: 新潟コンベンションセンター（新潟県・新潟市）
  • Year and Date: 2015-09-17
• [Presentation] 糖質コルチコイド受容体によるリガンド特異的転写活性におけるA/B領域の必要性 (2014)
  • Author(s): 岡香織、岡田大二朗、杉本章、勝義直
  • Organizer: 日本動物学会
  • Place of Presentation: 東北大学（仙台市）
  • Year and Date: 2014-09-11 – 2014-09-13
• [Remarks] 生殖発生生物学講座（1b）
  • URL: http://www.repdev-katsu.jp/index.html
• [Remarks] Press release (2016/8/22)
  • URL: http://www.hokudai.ac.jp/news/160822_sci_pr.pdf
• [Remarks] 北海道大学 生殖発生生物学講座1b
  • URL: http://www.repdev-katsu.jp/index.html