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The role of antimicrobial peptides on the function of the bursa of Faburicius: Do they bridge innate and adaptive immunity in avian?

Research Project

Project/Area Number 26440161
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphology/Structure
Research InstitutionSaitama University

Principal Investigator

KOBAYASHI Tetsuya  埼玉大学, 理工学研究科, 教授 (00195794)

Co-Investigator(Kenkyū-buntansha) 西垣 功一  埼玉大学, 理工学研究科, 教授 (10107378)
菊山 榮  早稲田大学, 教育・総合科学学術院, 名誉教授 (20063638)
岩室 祥一  東邦大学, 理学部, 教授 (70221794)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords抗菌ペプチド / ファブリキウス嚢 / ウズラ / fowlicidin
Outline of Final Research Achievements

We cloned a cDNA encoding the precursors of fowlicidin-1(f1), -2(f2), -3(f3) and cathelicidin-B1(cb1) from the quail bursa of Fabricius. In situ hybridization experiments demonstrated that these mRNAs were invariably detected in the inter-follicular epithelium. In addition, f2 and cb1 mRNAs were also detected in the follicles. A broth dilution analysis showed that the synthetic compounds f1 and f2 exerted potent antimicrobial activity against E. coli and S. aureus. A binding assay demonstrated that synthetic these peptides showed binding activity for the LPS. An electromobility shift assay revealed that these peptides also bound to the LTA. Enhancement of f3 and f1 mRNA expressions was noted following intravenous injections of LPS and LTA into the quail, respectively. Dexamethasone treatment suppressed the LPS-induced f3 mRNA expression. Female mice injected with ovalbumin and f1 or f2 showed higher ovalbumin-specific antiserum titers than female mice injected with ovalbumin alone.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (24 results)

All 2016 2015 2014

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (22 results) (of which Int'l Joint Research: 1 results) Book (1 results)

  • [Journal Article] Bacterial toxin-inducible gene expression of cathelicidin-B1 in the chicken bursal lymphoma-derived cell line DT40: Functional characterization of cathelicidin-B12014

    • Author(s)
      Takeda A, Tsubaki T, Sagae N, Onda Y, Inada Y, Mochizuki T, Okumura K, Kikuyama S, Kobayashi T, Iwamuro S.
    • Journal Title

      Peptides

      Volume: 59 Pages: 94-102

    • DOI

      10.1016/j.peptides.2014.06.012

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 魔弾の射手: 両生類の先天的防御機構.2016

    • Author(s)
      岩室祥一, 小林哲也.
    • Organizer
      第41回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      北里大学相模原キャンパス, 神奈川、相模原市
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] ニホンウズラ(Coturnix japonica)におけるβ-Defensinの遺伝子発現.2016

    • Author(s)
      ファムディン・ユイ,蓮沼 至,岩室祥一,菊山 榮,小林哲也.
    • Organizer
      第41回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      北里大学相模原キャンパス, 神奈川、相模原市
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] ウシガエル生体防御ペプチドcatesbeianalectinの脳内発現解析.2016

    • Author(s)
      中野真樹, 小林浩志, 杉山恵利香, 藤澤静香, 小林哲也, 菊山 榮, 蓮沼 至, 岩室祥一.
    • Organizer
      第41回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      北里大学相模原キャンパス, 神奈川、相模原市
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] リュウキュウアカガエル(Rana ulma)皮膚の生体防御ペプチド.2016

    • Author(s)
      小川大輔, 古川真帆, 持立真希, 小林哲也, 菊山 榮, 蓮沼 至, 岩室祥一.
    • Organizer
      第41回日本比較内分泌学会大会及びシンポジウム
    • Place of Presentation
      北里大学相模原キャンパス, 神奈川、相模原市
    • Year and Date
      2016-12-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] ウズラのファブリキウス嚢におけるfowlicidin-1, -2, -3 mRNAの発現.2016

    • Author(s)
      池田拓実, 近藤洋匡, 布村大樹, 蓮沼 至, 岩室祥一, 菊山 榮, 小林哲也.
    • Organizer
      第40回鳥類内分泌研究会
    • Place of Presentation
      ホテルメルパルク松山、愛媛、松山市
    • Year and Date
      2016-11-03
    • Related Report
      2016 Annual Research Report
  • [Presentation] Regulation of mRNA expression and biological effects of fowlicidin-1, -2, -3 in the quail (Coturnix japonica) bursa of Fabricius.2016

    • Author(s)
      Ikeda T, Kondo H, Nunomura D, Furudate H, Yamanaka N, Hasunuma I, Iwamuro S, Kikuyama S, Kobayashi T.
    • Organizer
      RegPep2016
    • Place of Presentation
      Rouen, France
    • Year and Date
      2016-07-12
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Expression of fowlicidin 1-3 mRNAs in the quail (Coturnix japonica) bursa of Fabricius.2015

    • Author(s)
      Ikeda T. Nunomura D. Kondo H. Furudate H. Hasunuma I. Iwamuro S. Kikuyama S. Kobayashi T.
    • Organizer
      CompBiol 2015 (第40回日本比較内分泌学会・第40回日本比較内分泌学会・日本比較生理生化学会第37回大会 合同大会)
    • Place of Presentation
      JMSアステールプラザ(広島県広島市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Involvement of Ca2+ influx in the TRH-induced RPL release from the pituitary gland of the bullfrog.2015

    • Author(s)
      Yamada K. Urabe A. Hashida Y. Minagawa A. Nakano M. Shindo H. Hasunuma I. Yamamoto K. Kikuyama S. Kobayashi T.
    • Organizer
      CompBiol 2015 (第40回日本比較内分泌学会・第40回日本比較内分泌学会・日本比較生理生化学会第37回大会 合同大会)
    • Place of Presentation
      JMSアステールプラザ(広島県広島市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Impaired lipid metabolism in growth-retarded mice.2015

    • Author(s)
      Ohara N. Kobayashi T.
    • Organizer
      CompBiol 2015 (第40回日本比較内分泌学会・第40回日本比較内分泌学会・日本比較生理生化学会第37回大会 合同大会)
    • Place of Presentation
      JMSアステールプラザ(広島県広島市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] Molecular cloning and expression analysis of gene encoding host defense peptide in amphibian skin cell lines with special reference to acyclic brevinin-1.2015

    • Author(s)
      Mizuta K. Ukaji S. Saito A. Tazato S. Hasunuma I. Kobayashi T. Kikuyama S. Iwamuro S.
    • Organizer
      CompBiol 2015 (第40回日本比較内分泌学会・第40回日本比較内分泌学会・日本比較生理生化学会第37回大会 合同大会)
    • Place of Presentation
      JMSアステールプラザ(広島県広島市)
    • Year and Date
      2015-12-11
    • Related Report
      2015 Research-status Report
  • [Presentation] ウズラのファブリキウス嚢における fowlicidin-3 の発現解析2015

    • Author(s)
      池田拓実、布村大樹、近藤洋匡、蓮沼 至、岩室祥一、菊山 榮、小林哲也
    • Organizer
      日本動物学会第86回新潟大会
    • Place of Presentation
      朱鷺メッセ:新潟コンベンションセンター(新潟県新潟市)
    • Year and Date
      2015-09-15
    • Related Report
      2015 Research-status Report
  • [Presentation] First success in selection of functional peptides using a novel concept microarray MMV-A breakthrough for cell-based screenings.2015

    • Author(s)
      Nishigaki K. Furukawa F. Takeuchi N. Saiki T. Hongo A. Iwano M. Masubuhi Y. Suzuki M
    • Organizer
      第53回日本生物物理学会年会
    • Place of Presentation
      金沢大学角間キャンパス(石川県金沢市)
    • Year and Date
      2015-09-13
    • Related Report
      2015 Research-status Report
  • [Presentation] ファブリキウス嚢における抗菌ペプチドfowlicidin-3の発現解析2015

    • Author(s)
      池田拓実,近藤洋匡,蓮沼至,岩室祥一,菊山榮,小林哲也
    • Organizer
      日本動物学会第67回関東支部大会
    • Place of Presentation
      早稲田大学・先端生命医科学センター, 東京
    • Year and Date
      2015-03-14
    • Related Report
      2014 Research-status Report
  • [Presentation] Differential modes of antimicrobial action of histones against Gram-negative and Gram-positive bacteria.2014

    • Author(s)
      岩室祥一,川名夏未,山中菜々子,稲田豊里,白石貴如,森田愁,多賀井千尋,川崎広明
    • Organizer
      第37回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜,横浜
    • Year and Date
      2014-11-25
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular cloning, localization analysis and multifunctional characterization of host defense peptides in the tongue of Rana catesbeiana.2014

    • Author(s)
      Shishido S, Omori S, Kondo H, Konishi Y, Sagae N, Hasunuma I, Iwamuro S, Kikuyama S, Kobayashi T.
    • Organizer
      The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      Okazaki Conference Center, Okazaki
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Identification of thyrotropin-releasing hormone receptor mediating the prolactin secretion in the bullfrog pituitary.2014

    • Author(s)
      Tetsuya Kobayashi, Masaki Nakano, Atsuko Minagawa, Shawichi Iwamuro, Kazutoshi Yamamoto, Itaru Hasunuma, Sakae Kikuyama.
    • Organizer
      The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      Okazaki Conference Center, Okazaki
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular cloning and multifunctional characterization of catesbeianalectin from the bullfrog Harderian gland.2014

    • Author(s)
      Shawichi Iwamuro, Yuki Konishi, Eri Uchiyama, Takuya Mochizuki, Erika Sugiyama, Itaru Hasunuma, Tetsuya Kobayashi, and Sakae Kikuyama.
    • Organizer
      The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
    • Place of Presentation
      Okazaki Conference Center, Okazaki
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Tissue expression and biological effects of fowlicidin-2 in the quail Bursa of Fabricius.2014

    • Author(s)
      近藤洋匡,武田あすな,蓮沼至,岩室祥一,菊山榮,古舘宏之,小林哲也
    • Organizer
      第39 回 日本比較内分泌学会大会・第8 回 国際両生類爬虫類神経内分泌学会 合同大会
    • Place of Presentation
      基礎生物学研究所岡崎コンファレンスセンター, 岡崎
    • Year and Date
      2014-11-07 – 2014-11-09
    • Related Report
      2014 Research-status Report
  • [Presentation] Histones as antimicrobial agents.2014

    • Author(s)
      岩室祥一,多賀井千尋,森田愁,白石貴如,川崎広明
    • Organizer
      第51回ペプチド討論会
    • Place of Presentation
      徳島大学,徳島
    • Year and Date
      2014-10-22 – 2014-10-24
    • Related Report
      2014 Research-status Report
  • [Presentation] MMV-4SR : Development of basic detection system in Single Cell Biology (SCB)2014

    • Author(s)
      竹内尚紀, 永野富郎, 西垣功一
    • Organizer
      生物物理学会第52回大会
    • Place of Presentation
      札幌コンベンションセンター, 札幌
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Research-status Report
  • [Presentation] ファブリキウス嚢におけるfowlicidin-2の生理的役割2014

    • Author(s)
      近藤洋匡,武田あすな,蓮沼至,岩室祥一,菊山榮,古舘宏之,小林哲也
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学, 仙台
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Presentation] 生体防御分子としてのヒストンH3の発現調節機構の解明2014

    • Author(s)
      稲田豊里,岩室祥一
    • Organizer
      日本動物学会第85回大会
    • Place of Presentation
      東北大学, 仙台
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Book] 「ホルモンから見た生命現象と進化」シリーズ第7巻「生体防御・社会性」、12章「生体防御ペプチドによる両生類の先天的防御機構」2016

    • Author(s)
      岩室祥一、小林哲也.
    • Total Pages
      258
    • Publisher
      裳華房
    • Related Report
      2016 Annual Research Report

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Published: 2014-04-04   Modified: 2018-03-22  

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