The role of antimicrobial peptides on the function of the bursa of Faburicius: Do they bridge innate and adaptive immunity in avian?
Project/Area Number |
26440161
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphology/Structure
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Research Institution | Saitama University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
西垣 功一 埼玉大学, 理工学研究科, 教授 (10107378)
菊山 榮 早稲田大学, 教育・総合科学学術院, 名誉教授 (20063638)
岩室 祥一 東邦大学, 理学部, 教授 (70221794)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 抗菌ペプチド / ファブリキウス嚢 / ウズラ / fowlicidin |
Outline of Final Research Achievements |
We cloned a cDNA encoding the precursors of fowlicidin-1(f1), -2(f2), -3(f3) and cathelicidin-B1(cb1) from the quail bursa of Fabricius. In situ hybridization experiments demonstrated that these mRNAs were invariably detected in the inter-follicular epithelium. In addition, f2 and cb1 mRNAs were also detected in the follicles. A broth dilution analysis showed that the synthetic compounds f1 and f2 exerted potent antimicrobial activity against E. coli and S. aureus. A binding assay demonstrated that synthetic these peptides showed binding activity for the LPS. An electromobility shift assay revealed that these peptides also bound to the LTA. Enhancement of f3 and f1 mRNA expressions was noted following intravenous injections of LPS and LTA into the quail, respectively. Dexamethasone treatment suppressed the LPS-induced f3 mRNA expression. Female mice injected with ovalbumin and f1 or f2 showed higher ovalbumin-specific antiserum titers than female mice injected with ovalbumin alone.
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Report
(4 results)
Research Products
(24 results)
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[Presentation] Regulation of mRNA expression and biological effects of fowlicidin-1, -2, -3 in the quail (Coturnix japonica) bursa of Fabricius.2016
Author(s)
Ikeda T, Kondo H, Nunomura D, Furudate H, Yamanaka N, Hasunuma I, Iwamuro S, Kikuyama S, Kobayashi T.
Organizer
RegPep2016
Place of Presentation
Rouen, France
Year and Date
2016-07-12
Related Report
Int'l Joint Research
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[Presentation] Molecular cloning, localization analysis and multifunctional characterization of host defense peptides in the tongue of Rana catesbeiana.2014
Author(s)
Shishido S, Omori S, Kondo H, Konishi Y, Sagae N, Hasunuma I, Iwamuro S, Kikuyama S, Kobayashi T.
Organizer
The 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology
Place of Presentation
Okazaki Conference Center, Okazaki
Year and Date
2014-11-07 – 2014-11-09
Related Report
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