Comparative study for gastrointestinal motor stimulating actions of ghrelin and motilin

• Project/Area Number: 26440169
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Rakuno Gakuen University
• Principal Investigator: KITAZAWA Takio 酪農学園大学, 獣医学群, 教授 (50146338)
• Co-Investigator(Kenkyū-buntansha): 海谷 啓之 国立研究開発法人国立循環器病研究センター, 研究所, 室長 (40300975) ; 寺岡 宏樹 酪農学園大学, 獣医学群, 教授 (50222146)
• Co-Investigator(Renkei-kenkyūsha): Sakai Takafumi 埼玉大学, 理工学研究科, 教授 (40235114)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: グレリン / モチリン / 消化管 / 収縮作用 / 脊椎動物 / グレリン受容体 / モチリン受容体 / 魚類 / 両生類 / 消化管運動 / 免疫組織化学 / 系統発生 / ゼブラフィッシュ / 鳥類 / 受容体

Outline of Final Research Achievements

Ghrelin and motilin are gut peptides that regulate the gastrointestinal motility of mammals and birds. The aim of this study is to clarify the function of motilin/ghrelin on gastrointestinal contractility of amphibians and fish using isolated muscle strips. Homologous ghrelin did not change the contractility of amphibians (bullfrog and Japanese fire belly newt) and fish (zebrafish) gastrointestinal tract, suggesting that the motor stimulating action of ghrelin is not conserved across vertebrates. On the other hand, motilin-mediated gastrointestinal motor regulating system is possible to present in the bullfrog and zebrafish but its physiological significance should be studied in future.

Research Products

• [Journal Article] Characterization of feline cytochrome P450 2B6. (2017)
  • Author(s): Okamatsu G, Komatsu T, Ono Y, Inoue H, Uchide T, Onaga T, Endoh D, Kitazawa T, Hiraga T, Uno Y, Teraoka H.
  • Journal Title: Xenobiotica. Volume: 47 Issue: 2 Pages: 93-102
  • DOI: 10.3109/00498254.2016.1145754
  • Peer Reviewed
• [Journal Article] Does motilin peptide regulate gastrointestinal motility of zebrafish? An in vitro study using isolated intestinal strips. (2017)
  • Author(s): Kitazawa T, Yoshida M, Teraoka H, Kaiya H.
  • Journal Title: General and Comparative Endocrinology Volume: 印刷中 Pages: 15-23
  • DOI: 10.1016/j.ygcen.2017.02.014
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Functional expression and comparative characterization of four feline P450 cytochromes using fluorescent substrates. (2017)
  • Author(s): Okamatsu G, Kawakami K, Komatsu T, Kitazawa T, Uno Y, Teraoka H.
  • Journal Title: Xenobiotica. Volume: 印刷中 Issue: 11 Pages: 951-961
  • DOI: 10.1080/00498254.2016.1257172
  • Peer Reviewed
• [Journal Article] Neuropeptide Y (NPY) inhibits spontaneous contraction of the mouse atrium by possible activation of NPY1 receptor (2017)
  • Author(s): Oki Y, Teraoka H, Kitazawa T
  • Journal Title: Autonomic and Autacoid Pharmacology Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Molecular cloning of motilin and mechanism of motilin-induced gastrointestinal motility in Japanese quail (2016)
  • Author(s): Auvijit Saha Apu, Anupom Mondal, Takio Kitazawa, Shota Takemi, Takafumi Sakai, Ichiro Sakata
  • Journal Title: General and Comparative Endocrinology Volume: 233 Pages: 53-62
  • DOI: 10.1016/j.ygcen.2016.05.017
  • NAID: 120006666537
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Protective effect of prostacyclin against pre-cardiac edema caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin and a thromboxane receptor agonist in developing zebrafish (2016)
  • Author(s): Nijoukubo, D., Tanaka, Y., Okuno, Y., Yin, G., Kitazawa, T., Peterson, R. E., Kubota, A. and Teraoka, H.
  • Journal Title: Chemosphere Volume: 156 Pages: 111-117
  • DOI: 10.1016/j.chemosphere.2016.04.107
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of ghrelin and motilin on smooth muscle contractility of the isolated gastrointestinal tract from the bullfrog and Japanese fire belly newt. (2016)
  • Author(s): Kitazawa T, Shimazaki M, Kikuta A, Yaosaka N, Teraoka H, Kaiya H.
  • Journal Title: Gen Comp Endocrinology Volume: 232 Pages: 51-59
  • DOI: 10.1016/j.ygcen.2015.12.013
  • NAID: 120006666510
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Identification and functional characterization of novel feline cytochrome P450 2A. (2015)
  • Author(s): Okamatsu G, Komatsu T, Kubota A, Onaga T, Uchide T, Endo D, Kirisawa R, Yin G, Inoue H, Kitazawa T, Uno Y, Teraoka H.
  • Journal Title: Xenobiotica Volume: 45 Issue: 6 Pages: 503-510
  • DOI: 10.3109/00498254.2014.998322
  • Peer Reviewed
• [Journal Article] Accumulation properties of inorganic mercury and organic mercury in the red-crowned crane Grus japonensis in east Hokkaido, Japan. (2015)
  • Author(s): Teraoka H, Okamoto E, Kudo M, Nakayama SM, Ikenaka Y, Ishizuka M, Endo T, Kitazawa T, Hiraga T.
  • Journal Title: Ecotoxicol Environ Saf. Volume: 122 Pages: 557-564
  • DOI: 10.1016/j.ecoenv.2015.09.025
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Correlation of ghrelin concentration and ghrelin, ghrelin-O-acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken. (2015)
  • Author(s): Kitazawa T, Hiraga T, Teraoka H, Yaosaka N. and Kaiya H
  • Journal Title: Peptides Volume: 63 Pages: 134-142
  • DOI: 10.1016/j.peptides.2014.11.006
  • NAID: 120006311881
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Involvement of COX2-thromboxane pathway in TCDD-induced precardiac edema in developing zebrafish (2014)
  • Author(s): Teraoka H, Okuno Y, Nijoukubo D, Yamakoshi A, Peterson R, Stegeman JJ, Kitazawa T, Hiraga T. and Kubota A.
  • Journal Title: Auat Toxicol. Volume: 154 Pages: 19-26
  • DOI: 10.1016/j.aquatox.2014.04.025
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] ムスカリン受容体を介した胃粘膜細胞の分化・増殖制御 (2016)
  • Author(s): 松山勇人、内藤清惟、和田善明、永野宏、斉藤正一郎、酒井洋樹、棚橋靖行、北澤多喜雄、小森成一、海野年弘
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（藤沢校舎）
  • Year and Date: 2016-09-05
• [Presentation] Possible regulation of gastrointestinal motility by motilin in zebrafish -an in vitro study using isolated muscle strips (2016)
  • Author(s): Kitazawa T, Teraoka H, Kaiya H
  • Organizer: Regukatiry Peptide 2016(REGPEP2016)
  • Place of Presentation: Rouen, France
  • Year and Date: 2016-07-12
  • Int'l Joint Research
• [Presentation] What is the general action of ghrelin in vertebrates? Study focused on gastrointestinal motor action (2016)
  • Author(s): ○北澤多喜雄、寺岡宏樹、海谷啓之
  • Organizer: 第93回日本生理学会
  • Place of Presentation: 札幌コンベンションセンター（札幌）
  • Year and Date: 2016-03-24
• [Presentation] ウシガエルとイモリの胃腸管収縮に対するグレリンの効果 (2015)
  • Author(s): 北澤多喜雄、嶋崎未里、菊田 歩、八百坂 紀子、寺岡宏樹、○海谷啓之
  • Organizer: 日本動物学会 第86回 大会 2015新潟
  • Place of Presentation: 朱鷺メッセ、新潟コンベンションセンター（新潟）
  • Year and Date: 2015-09-19
• [Presentation] ウシガエルの消化管収縮におよぼすghrelinおよびmotilinの影響 (2015)
  • Author(s): ○北澤多喜雄、嶋崎美里、寺岡宏樹、海谷啓之
  • Organizer: 第57回日本平滑筋学会
  • Place of Presentation: 山口大学医学部（宇部市）
  • Year and Date: 2015-08-27
• [Presentation] Chicken is a specific and useful animal model to study the functional role of ghrelin and motilin in regulation of gastrointestinal motility. (2014)
  • Author(s): Kitazawa T, Teraoka H and Kaiya H
  • Organizer: 20th International Symposium on Regulatory Peptides
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2014-09-07 – 2014-09-10
• [Book] Neuromethods Vol. 107. Muscarinic receptor. From Structure to Animal Models.Chapter 13. Regulation of heart contractility by M2 and M3 muscarinic receptors: functional studies using muscarinic receptor knockout mouse. Myslivecek J and Jakublk J (eds) (2015)
  • Author(s): Kitazawa T, Teraoka H, Harada N, Ochi K, Nakamura T, Asakawa K, Kanegae S, Yaosaka N, Unno T, Komori S and Yamada M.
  • Total Pages: 281
  • Publisher: Springer Science+Business Media New York.