| Project/Area Number |
26450002
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Science in genetics and breeding
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| Research Institution | Mie University |
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Principal Investigator |
Tsuchiya Tohru 三重大学, 地域イノベーション推進機構, 准教授 (30293806)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 自家不和合性 / サツマイモ野生種 / 自他認識 / S遺伝子 / 自他認識機構 / 高等植物 / 生殖生理 |
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| Outline of Final Research Achievements |
Ipomoea trifida have sporophytic self-incompatibility system. In I.trifida, AB2 gene were identified as male S-candidate gene, SE2 and SEA genes were identified as female S-candidate genes. To determine male S-gene of I.trifida, S1-AB2 peptides were chemically synthesized and applied to S1 homozygous stigma before pollination with S3 pollen. According to this bioassay, it is strongly suggested that AB2 may act as male S-gene, because pollinated S3 pollen were not germinated on the S1 stigma. To determine female S-gene of I.trifida, S1-S candidate genes were introduced into S10 homozygous I.trifida, however, S-gene was not determined by this experiment because of low fertility of transgenic pollen grains. To determine S-genes of I.trifida, comparative analysis were carried out with S-loci sequences from 4 self-incompatible I.trifida lines and 2 self-compatible I.trifida and I.nil lines. From this analysis, AB2 and SEA genes were identified as predominant genes for S-genes in I.trifida.
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