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Map-based cloning and function analysis of a wheat vernalization gene Vrn-D4

Research Project

Project/Area Number 26450005
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Science in genetics and breeding
Research InstitutionOkayama University

Principal Investigator

Nishida Hidetaka  岡山大学, 環境生命科学研究科, 准教授 (30379820)

Research Collaborator KATO Kenji  
DUBCOVSKY Jorge  
KIPPES Nestor  
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsコムギ / 春播性遺伝子 / Vrn-D4 / クローニング / Vrn-A1 / 起源 / 重複配列 / テロソミック / シンテニー / 相互作用
Outline of Final Research Achievements

In the previous study, a wheat vernalization gene Vrn-D4 was mapped in a centromeric region, 0.09cM interval, of chromosome 5D. In this study, Vrn-D4 was found to be located on the short arm. This new information narrowed further down the Vrn-D4 region. In another analysis, the line with a spring allele Vrn-D4 was found to carry two copies of Vrn-A1, one of which was located on the short arm of chromosome 5D and had specific polymorphisms. Other lines with a spring allele Vrn-D4 also had this Vrn-A1 copy, which confirmed that the causal gene of Vrn-D4 is the Vrn-A1 copy on the short arm of chromosome 5D. Moreover, origin of Vrn-D4 was analyzed.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (7 results)

All 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (4 results)

  • [Int'l Joint Research] カリフォルニア大学デービス校(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] カリフォルニア大学デービス校(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Fine mapping and epistatic interactions of the vernalization gene VRN-D4 in hexaploid wheat.2014

    • Author(s)
      Kippes N, Zhu J, Chen A, Vanzetti L, Lukaszewski A, Nishida H, Kato K, Dvorak J, Dubcovsky J
    • Journal Title

      Molecular Genetics and Genomics

      Volume: 289 Issue: 1 Pages: 47-62

    • DOI

      10.1007/s00438-013-0788-y

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] コムギにおける春播性遺伝子Vrn-A1コピーの多様性とVrn-D4の起源2016

    • Author(s)
      原田英樹・西田英隆・加藤鎌司
    • Organizer
      第8回中国地域育種談話会・第11回ムギ類研究会(共催)
    • Place of Presentation
      岡山大学資源植物科学研究所(倉敷市)
    • Year and Date
      2016-12-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] コムギにおける春播性遺伝子Vrn-D4の起源の解明を目指して2015

    • Author(s)
      原田英樹,西田英隆,加藤鎌司
    • Organizer
      第7回 中国地域育種談話会
    • Place of Presentation
      岡山市(岡山大学)
    • Year and Date
      2015-12-19
    • Related Report
      2015 Research-status Report
  • [Presentation] コムギにおける春播性遺伝子Vrn-D4のクローニング及びその起源に関する考察2015

    • Author(s)
      加藤鎌司,N. Kippes,原田英樹,西田英隆,J. Dubcovsky
    • Organizer
      日本育種学会 第128回講演会
    • Place of Presentation
      新潟市(新潟大学)
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] 中国のコムギ在来品種におけるVrn-D4遺伝子型の再検討2015

    • Author(s)
      加藤鎌司・原田英樹・西田英隆
    • Organizer
      日本育種学会 第127回講演会
    • Place of Presentation
      玉川大学(町田市)
    • Year and Date
      2015-03-21 – 2015-03-22
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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