Identification of genes regulating seed size in rice

Research Project

Project/Area Number	26450008
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Science in genetics and breeding
Research Institution	Fukui Prefectural University
Principal Investigator	IWASAKI Yukimoto 福井県立大学, 生物資源学部, 教授 (20193732)
Research Collaborator	MIURA Kotaro 福井県立大学, 生物資源学部, 准教授 (70571561)
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000) Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords	イネ / 種子形 / 粒形変異体 / ３量体Gタンパク質 / ブラシノステロイド / ３量体Ｇタンパク質
Outline of Final Research Achievements	In order to understand mechanism regulating seed size, the identification and isolation of genes regulating seed size using novel seed size mutants in rice, srs2 and srs6 were carried out. A SNP due to nonsense mutation was detected in an orthologue gene to Arabidopsis BRASSINOSTEROID-SIGNALING KINASE 2 (BSK2) in srs2 mutant. A one base substitution due to generate a stop codon was found ERECT PANICLE 3 (EP3) gene, which encodes F-box protein in srs6 mutant. Now we start the complementation test introducing wild type genes and produce the knock out rice using CRISPR-Cas 9 system, in order to proof the function of the candidate genes.