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Analysis of genetic relationships of citrus and its relatives by next generation sequencer

Research Project

Project/Area Number 26450039
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Horticultural science
Research InstitutionSaga University

Principal Investigator

Nagano Yukio  佐賀大学, 総合分析実験センター, 准教授 (00263038)

Co-Investigator(Kenkyū-buntansha) 山本 雅史  鹿児島大学, 農水産獣医学域農学系, 教授 (00305161)
古藤田 信博  佐賀大学, 農学部, 准教授 (50355426)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsカンキツ / ヒマラヤンライム / ゲノム / 次世代シーケンサー / カンキツ近縁属 / 遺伝資源 / RAD-Seq / メキシカンライム / 遺伝子資源 / RAD-seq
Outline of Final Research Achievements

We examined the genetic relationship between various citrus fruits, by analyzing DNA using cutting-edge analytical instrument "Next Generation Sequencer." As a result, we reconfirmed the known findings more strictly, and described new findings. Particular finding is the discovery of the new citrus fruit, which was designated as “Himalayan lime.” Himalayan lime found in Bhutan is morphologically similar to Mexican lime, which is known all over the world. However, it was different from Mexican Lime when we examined its DNA.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (24 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (13 results) (of which Int'l Joint Research: 3 results) Remarks (2 results)

  • [Int'l Joint Research] 再生可能自然資源研究センター(ブータン)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] 雲南農業大学(中国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] RAD-Seq analysis of typical and minor <i>Citrus</i> accessions, including Bhutanese varieties2016

    • Author(s)
      Penjor T, Mimura T, Kotoda N, Matsumoto R, Nagano AJ, Honjo MN, Kudoh H, Yamamoto M, Nagano Y.
    • Journal Title

      Breeding Science

      Volume: 66 Issue: 5 Pages: 797-807

    • DOI

      10.1270/jsbbs.16059

    • NAID

      130005290452

    • ISSN
      1344-7610, 1347-3735
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Arabidopsis LBP/BPI related-1 and-2 bind to LPS directly and regulate PR1 expression2016

    • Author(s)
      Sayaka Iizasa, Ei'ichi Iizasa, Sawako Matsuzaki, Hiroyuki Tanaka, Yutaka Kodama,Keiichi Watanabe, Yukio Nagano
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 27527-27527

    • DOI

      10.1038/srep27527

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Complete Genome Sequence of Bacillus thuringiensis Serovar Tolworthi Strain Pasteur Institute Standard2015

    • Author(s)
      Kohzo Kanda, Kaede Nakashima, Yukio Nagano
    • Journal Title

      Genome Announcements

      Volume: 3 Issue: 4

    • DOI

      10.1128/genomea.00710-15

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 相同組換えで色々できちゃうDNAクローニング2015

    • Author(s)
      永野幸生・飯笹英一
    • Journal Title

      生物工学会誌

      Volume: 93 Pages: 623-626

    • Related Report
      2015 Research-status Report
  • [Journal Article] Characterization of limes (Citrus aurantifolia) grown in Bhutan and Indonesia using high-throughput sequencing2014

    • Author(s)
      Tshering Penjor, Takashi Mimura, Ryoji Matsumoto, Masashi Yamamoto, Yukio Nagano
    • Journal Title

      Sci. Rep

      Volume: 4 Issue: 1 Pages: 4853-4853

    • DOI

      10.1038/srep04853

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Exploration of Local Citrus Genetic Resources in Bhutan and their Chloroplast DNA Analysis2014

    • Author(s)
      Tshering Penjor, 永野幸生, 三村高史, 松本亮司, 山本雅史
    • Journal Title

      Horticultural Research (Japan)

      Volume: 13 Issue: 4 Pages: 307-314

    • DOI

      10.2503/hrj.13.307

    • NAID

      130004938804

    • ISSN
      1347-2658, 1880-3571
    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of chloroplast matK sequences of Citrus tachibana and Citrus depressa, two indigenous species in Japan2014

    • Author(s)
      Yukio Nagano, Sayuri Inafuku-Teramoto, Misako Hashimoto, Takashi Mimura, Ryoji Matsumoto, Masashi Yamamoto
    • Journal Title

      Advances in Horticultural Science

      Volume: 28 Pages: 95-99

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Presentation] LPS誘導性遺伝子群のジーンオントロジー解析におけるArabidopsis LBP/BPI related-2 (AtLBR-2)の重要性について2017

    • Author(s)
      飯笹さやか, 飯笹英一, 渡邉啓一, 永野幸生
    • Organizer
      第58回日本植物生理学会年会
    • Place of Presentation
      鹿児島大学
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
  • [Presentation] Himalayan lime, a new citrus variety found in Bhutan2016

    • Author(s)
      Yukio Nagano
    • Organizer
      Third Myanmar-Japan International Symposium
    • Place of Presentation
      Pathein University, Myanmar
    • Year and Date
      2016-12-03
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Arabidopsis LBP/BPI related-2 (AtLBR-2) binds to LPS directly and regulates LPS-induced defense responses2016

    • Author(s)
      Sayaka Iizasa, Ei'ichi Iizasa, Keiichi Watanabe,Yukio Nagano
    • Organizer
      The Cold Spring Harbor Asia conference on Latest Advances in Plant Development & Environmental Responses
    • Place of Presentation
      淡路夢舞台国際会議場
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] RAD-Seq analysis of Citrus species2016

    • Author(s)
      Yukio Nagano
    • Organizer
      The 3rd International Workshop for Conservation Genetics of Mangroves
    • Place of Presentation
      琉球大学・西表研究施設
    • Year and Date
      2016-10-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 完全配列を決定した Bacillus thuringiensis subsp. tolworthi のプロファージと転位領域の特徴づけ2016

    • Author(s)
      石動丸文子, 神田康三, 永野幸生
    • Organizer
      日本農芸化学会2016年度西日本支部大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2016-09-15
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒマラヤンライム等のブータンで発見した興味深いカンキツ遺伝資源2016

    • Author(s)
      永野幸生, Tshering Penjor, 三村高史, 古藤田信博, 松本亮司, 永野惇, 本庄三恵, 工藤洋, 山本雅史
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 中国雲南省におけるカンキツ遺伝資源の調査2016

    • Author(s)
      山本雅史, 楊学虎, 北島宣, 永野幸生
    • Organizer
      園芸学会
    • Place of Presentation
      名城大学
    • Year and Date
      2016-09-10
    • Related Report
      2016 Annual Research Report
  • [Presentation] 苦労続きのバチルス・チューリンゲンシス亜型tolworthi の全ゲノム配列決定2015

    • Author(s)
      永野 幸生, 中嶋 楓, 神田 康三
    • Organizer
      NGS現場の会 第四回研究会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2015-07-01
    • Related Report
      2015 Research-status Report
  • [Presentation] ヘテロ接合性喪失による無性生殖するカンキツの遺伝的分化2015

    • Author(s)
      永野幸生・Tshering Penjor・三村高史・松本亮司・山本雅史
    • Organizer
      日本農芸化学会2015年度大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Research-status Report
  • [Presentation] Loss of heterozygosity as a possible mechanism of genetic diversification of Citrus species2014

    • Author(s)
      Yukio Nagano, Tshering Penjor, Takashi Mimura, Ryoji Matsumoto, Masashi Yamamoto
    • Organizer
      第37回日本分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2014-11-25 – 2014-11-27
    • Related Report
      2014 Research-status Report
  • [Presentation] 無性生殖するライムで遺伝的分化がおこる仕組みとしての「ヘテロ接合性喪失」2014

    • Author(s)
      永野幸生・Tshering Penjor・三村高史・松本亮司・山本雅史
    • Organizer
      園芸学会平成26年度秋季大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-27 – 2014-09-29
    • Related Report
      2014 Research-status Report
  • [Presentation] カンキツとがんの遺伝的分化機構の類似性2014

    • Author(s)
      永野幸生・Tshering Penjor・三村高史・松本亮司・山本雅史
    • Organizer
      2014年度日本農芸化学会西日本支部大会
    • Place of Presentation
      佐賀大学
    • Year and Date
      2014-09-18 – 2014-09-19
    • Related Report
      2014 Research-status Report
  • [Presentation] Gap Repair2014

    • Author(s)
      永野幸生
    • Organizer
      第38回蛋白質と酵素の構造と機能に関する九州シンポジウム
    • Place of Presentation
      レイクサイドホテル久山
    • Year and Date
      2014-09-11 – 2014-09-13
    • Related Report
      2014 Research-status Report
  • [Remarks] ブータンとインドネシアで生育しているライムの、ハイスループット・シークエンサーによる特徴づけ

    • URL

      http://www.iac.saga-u.ac.jp/lifescience/webpage_for_lime/index.html

    • Related Report
      2014 Research-status Report
  • [Remarks] クローンで増えるカンキツの識別技術の開発

    • URL

      http://www.iac.saga-u.ac.jp/lifescience/webpage_for_lime/for_the_public.html

    • Related Report
      2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-02-16  

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