Functional aspects of bacterial multiple transporter genes for aromatic compounds
| Project/Area Number |
26450087
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Shizuoka University |
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Principal Investigator |
Ogawa Naoto 静岡大学, 農学部, 教授 (60354031)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | トランスポーター / 芳香族化合物 / 応用微生物 / 細菌 / 取り込み / MFS family / 発現制御 |
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| Outline of Final Research Achievements |
The analysis of gene expression in Burkholderia multivorans ATCC17616 revealed different expression patterns of related transporter genes of Major Facilitator Super Family (MFS) for aromatic compounds such as benzoate and 3-chlorobenzoate (3CB). While benK2 was induced by benzoate and 3CB, benK3 was expressed constitutively. On the other hand, benK4 was induced by 3CB strongly. This result and the fact that benK4 gene constitutes gene cluster together with 3CB degradation genes suggested strongly that benK4 is involved in uptake of 3CB. Another MFS family member, mhbK constitute gene cluster with 3-hydroxybenzoate (3OH-Ben) degradation genes which seemed to be expressed by 3OH-Ben and was found to be involved in uptake of 3OH-Ben. These results suggested that the efficient expression of transporter gene is critical for the uptake of aromatic compound.
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Report
(4 results)
Research Products
(2 results)
