The search for novel biomarkers by comprehensive identification of human lipid modified proteins
| Project/Area Number |
26450125
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | N-ミリストイル化 / プレニル化 / バイオマーカー探索 / 網羅的探索 / タンパク質脂質修飾 / タンパク質翻訳後修飾 / 細胞情報伝達 / 脂質修飾 / 網羅的解析 |
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| Outline of Final Research Achievements |
In this study, to search for novel lipid modified proteins that can be applied for molecular biomarkers, we have established the strategy to identify the novel lipid modified proteins from human proteome by using metabolic labeling method performed in the insect cell-free protein synthesis system. By using this strategy, we next tried the comprehensive identification of human N-myristoylated proteins from whole human proteins listed in UniProt protein database. As a result, 171 novel N-myristoylated proteins were found from all human proteins. These novel N-myristoylated proteins contain not only physiologically important proteins mainly involved in the signal transduction pathways but also many disease-associated proteins that can be applied for molecular biomarkers.
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Report
(4 results)
Research Products
(31 results)
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[Journal Article] Phytoestrogen Suppresses Efflux of the Diagnostic Marker Protoporphyrin IX in Lung Carcinoma.2016
Author(s)
Fujita H, Nagakawa K, Kobuchi H, Ogino T, Kondo Y, Inoue K, Shuin T, Utsumi T, Utsumi K, Sasaki J, Ohuchi H.
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Journal Title
Cancer Res
Volume: 76
Issue: 7
Pages: 1837-1846
DOI
Related Report
Peer Reviewed
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[Journal Article] Homozygous mutation of STXBP5L explains an autosomal recessive infantile-onset neurodegenerative disorder.2015
Author(s)
R. Kumar, M.A. Corbett, N.JC. Smith, L.A. Jolly, C. Tan, D.J. Keating, M.D. Duffield, T. Utsumi, K. Moriya, K.R. Smith, A. Hoischen, K. Abbott, M.G. Harbord, A.G. Compton, J.A. Woenig, P. Arts, M. Kwint, N. Wieskamp, S. Gijsen, J.A. Veltman, M. Bahlo, J.G. Gleeson, E. Haan, J. Gecz.
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Journal Title
Human Molecular Genetics
Volume: 24
Issue: 7
Pages: 2000-2010
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] A Cell-Free Translocation System Using Extracts of Cultured Insect Cells to Yield Functional Membrane Proteins2014
Author(s)
Toru E., Kei N., Yoko S., Satomi S., Keishi A., Satoshi S., Masaaki I., Takahiro H., Gunnar V. H., Toshihiko U., Keietsu A., Eiji A. and Nobuyuki U.
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Journal Title
Pros One
Volume: 9
Issue: 12
Pages: e112874-e112874
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] ヘム代謝制御に基づく光感受性物質protoporphyrin IX蓄積機構の研究2016
Author(s)
藤田洋史, 永川恵介, 小渕浩嗣, 荻野哲也, 近藤洋一, 井上 啓史, 執印太郎, 内海俊彦, 内海耕慥, 佐々木順造, 大内淑代
Organizer
第 69 回日本酸化ストレス学会学術集会
Place of Presentation
仙台国際センター（宮城県仙台市）
Year and Date
2016-08-30
Related Report
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[Presentation] Lipidation of Arabidopsis CPK6 promotes the plasma membrane targeting and the stomata closure mediated by activation of SLAC1 in Arabidopsis guard cells.2016
Author(s)
Saito S, Hamamoto S, Sato Y, Uozumi N, Hashimoto K, Kudla J, Utsumi T, Moriya K, Tozawa Y, and Yamauchi S.
Organizer
International workshop on Plant Membrane Biology
Place of Presentation
Annapolis, USA
Year and Date
2016-06-05
Related Report
Int'l Joint Research
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