The study on the functions of hormones in the gonad.

• Project/Area Number: 26450285
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Hiroshima Institute of Technology (2016-2017); Ehime University (2014-2015)
• Principal Investigator: Miura Chiemi 広島工業大学, 環境学部, 教授 (90518002)
• Research Collaborator: KITAMURA Shu , 大学院生 ; HAYASHI Daichi , 大学院生
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 配偶子形成 / 成長 / 成長ホルモン / エストラジオール17β / 生殖巣 / 性成熟 / ニホンウナギ / 体細胞 / 精巣 / 卵巣

Outline of Final Research Achievements

Relationship between somatic growth and germ cells are unknown. In this study, we checked the growth in male and female Japanese eels, and the expression of growth control factor in pituitary gland, liver and　gonad. The results showed that, in both sexes, the maturity progressed with somatic growth. Also, a correlation with the expression of the growth control factor was shown.
In addition, the results suggested a possible role for the sex hormone, 17β-estradiol as one of the growth control factors, because the expression of growth control factor in the ovary varied with sex hormone in an in vitro experiment. The removal of gonad of the tilapia affected its growth. Treatment of gonadectomized fish with sex hormone influence its growth, and these effects are dependent on the stage of the gonad.

Research Products

• [Journal Article] Effect of the squid viscera hydrolysate on growth performance and digestion in the red sea bream Pagrus major (2017)
  • Author(s): Kondo Fumitaka、Ohta Takashi、Iwai Toshiharu、Ido Atsushi、Miura Chiemi、Miura Takeshi
  • Journal Title: Fish Physiol. Biochem. Volume: 43 Issue: 6 Pages: 1543-1555
  • DOI: 10.1007/s10695-017-0391-y
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 昆虫由来免疫賦活化物質「シルクロース」の魚類養殖への利用. (2017)
  • Author(s): 三浦猛, 井戸篤史, 橋爪篤史, 三浦智恵美
  • Journal Title: アクアネット Volume: 20(5) Pages: 48-54
• [Journal Article] 機能性多糖「シルクロース」による養殖魚介類の免疫機能向上と肉質改善. (2017)
  • Author(s): 三浦猛, 井戸篤史, 橋爪篤史, 高橋隆行, 三浦智恵美
  • Journal Title: 養殖ビジネス Volume: 54(10) Pages: 37-39
• [Journal Article] Analysis of feeding effects of EP on growth and digestion in cultured bluefin tuna (2016)
  • Author(s): 近藤史崇, 岩井俊治，三浦智恵美，坂田潤弥，太田史，井戸篤史， 入江奨，岡松一樹，角正浩一，三浦 猛
  • Journal Title: NIPPON SUISAN GAKKAISHI Volume: 82 Issue: 6 Pages: 923-933
  • DOI: 10.2331/suisan.16-00030
  • NAID: 130005284176
  • ISSN: 0021-5392, 1349-998X
  • Peer Reviewed
• [Journal Article] Silkrose: A novel acidic polysaccharide from the silkmoth that can stimulate the innate immune response. Carbohydr. Polym. (2016)
  • Author(s): Ohta, T., Kusano, K., Ido, A., Miura, C. and Miura, T.
  • Journal Title: Carbohydr. Polym. Volume: 136 Pages: 995-1001
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Dietary effects of housefly (Musca domestica) (Diptera: Muscidae) pupae on the growth performance and the resistance against bacterial pathogen in red sea bream (Pagrus major) (Perciformes: Sparidae). (2015)
  • Author(s): Ido, A., Iwai, T., Ito, K., Ohta, T., Mizushige, T., Kishida, T., Miura, C. and Miura, T.
  • Journal Title: Appl. Entomol. Zool. Volume: 50 Pages: 213-221
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] A novel polysaccharide in insects actives the innate immune system in mouse macrophage RAW 264 cells. (2014)
  • Author(s): Ohta, T., Ido, A., Kusano, K., Miura, C. and Miura, T.
  • Journal Title: PLOS ONE Volume: 9(12) Issue: 12 Pages: e114823-e114823
  • DOI: 10.1371/journal.pone.0114823
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Controlled feeding alleviates the recduced growth associated with spawning in farmed yellowtail (Seriola quinqueradiata). (2014)
  • Author(s): Miura, C., Yoshihara, Y., Shimizu-Yamaguchi, S., Hayashi, D., Hamada, K., Takeda, Y. Miura, M., Miura, T.
  • Journal Title: Aquaculture Volume: 424-425 Pages: 10-17
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Environmental condiditions of water body is the important factors in the growth of Akoya pearl oyster (Pinctada fucata) (2017)
  • Author(s): Chiemi Miura, Toshiharu Iwai, Tomohisa Konishi, Yoshinari Oguro Atsushi Ido and Takeshi Miura
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] The difference in substitution for fish meal with mealworm (Tenebrio molitor) in diet for red seabream (Pargus major) and amberjack (Seriola quinqueradiata). (2017)
  • Author(s): Atsushi Ido, Atsushi Hashizume, Chiemi Miura, Takeshi Miura.
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] Potential of housefly larva (Musca domestica) in sole replacement of fish meal; ingredient analysis and feeding trial in young red seabream (Pagrus major) (2017)
  • Author(s): Serigne Thierno Thiaw, Atsushi Hashizume, Atsushi Ido, Takayuki Takahashi, Chiemi Miura and Takeshi Miura
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] Protection mechanism of silkrose for bacterial pathogen infection in medaka (Oryzias latipes ) by comprehensive gene analysis (2017)
  • Author(s): Muhammad Fariz Zahir Ali , Kenta Kameda , Fumitaka Kondo , Atsushi Hashizume , Toshiharu Iwai, Chiemi Miura and Takeshi Miura
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] Silkrose is effective for vibriosis of Japanese tiger prawn (Marsupenaeus japonicas ): evidence from challenge study (2017)
  • Author(s): Indri Afriani Yasin , Takashi Ohta , Atsushi Hashizume , Atsushi Ido , Chiemi Miura Takeshi Miura
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium “Fisheries Science for Future Generations”
  • Int'l Joint Research
• [Presentation] Hemocytes play important roles in the pearl sac formation. (2017)
  • Author(s): Chiemi Miura, Toshiharu Iwai and Takeshi Miura
  • Organizer: The 14th International Symposium on Biomineralization (BIOMIN XIV)
  • Int'l Joint Research
• [Presentation] Analysis of the relationship of the hemocytes and epithelial cells of pearl oyster in pearl sac formation. (2016)
  • Author(s): Chiemi Miura, Toshiharu Iwai, Takeshi Miura
  • Organizer: The 87th Meeting of The Zoological Society of Japan
  • Place of Presentation: Okinawa Convention Center,Ginowan,Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Tidal current is one of the most important factors in the growth of Akoya pearl oyster. (2016)
  • Author(s): Takeshi Miura, Toshiharu Iwai, Ren Wakaki, Chiemi Miura
  • Organizer: The 87th Meeting of The Zoological Society of Japan
  • Place of Presentation: Okinawa Convention Center,Ginowan,Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] Bidirectional sex changes in Akoya pearl oyster. (2016)
  • Author(s): Toshiharu Iwai, Masaharu Takahashi, Jeane S. Mulyana, Chiemi Miura, Takeshi Miura
  • Organizer: The 87th Meeting of The Zoological Society of Japan
  • Place of Presentation: Okinawa Convention Center,Ginowan,Okinawa
  • Year and Date: 2016-11-17
  • Int'l Joint Research
• [Presentation] 黒潮海域サンゴ群生地でのオニヒトデの駆除と利用 (2015)
  • Author(s): 三浦智恵美・三宅開・太田史・目崎拓真・中地シュウ・三浦猛
  • Organizer: 中国四国地区生物系三学会
  • Place of Presentation: 愛媛大学(愛媛県・松山市）
  • Year and Date: 2015-05-16
• [Presentation] ニホンウナギの雌雄の成長差と生殖腺の相互関係 (2015)
  • Author(s): 北村脩・三浦智恵美・太田史・三浦猛
  • Organizer: 日本水産学会春季大会
  • Place of Presentation: 東京海洋大学（ 東京都・港区）
  • Year and Date: 2015-03-28 – 2015-03-31
• [Presentation] 天蚕サナギから得られた新規物質シルクロース (2014)
  • Author(s): 三浦智恵美・太田史・草野希枝・井戸篤史・三浦猛
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 九州大学（ 福岡県・福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] 養殖ブリにおける性成熟と骨との関係 (2014)
  • Author(s): 林大地・岩井俊治・三浦智恵美・三浦猛
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 九州大学（ 福岡県・福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] 脱Cdイカ内臓による養殖魚の 飼料効率改善効果の生理学的解析 (2014)
  • Author(s): 井戸篤史・太田史・岩井俊治・近藤史崇・ 三浦智恵美・三浦猛
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 九州大学（ 福岡県・福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] 養殖クロマグロの配合飼料に対する消化特性 (2014)
  • Author(s): 近藤史崇・岩井俊治・三浦智恵美・角正浩・三浦猛
  • Organizer: 日本水産学会秋季大会
  • Place of Presentation: 九州大学（ 福岡県・福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] ウリミバエサナギ由来免疫賦活化酸性多糖類Dipteroseの単離・同定 (2014)
  • Author(s): 太田史・草野希枝・井戸篤史・三浦智恵美・三浦猛
  • Organizer: 本水産学会秋季大会
  • Place of Presentation: 九州大学（ 福岡県・福岡市）
  • Year and Date: 2014-09-19 – 2014-09-22
• [Presentation] Controlled feeding rescues the growth reduction associated with spawning in farmed yellowtail (Seriola quinqueradiata) (2014)
  • Author(s): Miura, C., Yoshihara, Y., Hayashi, D. and Miura, T.
  • Organizer: 10th International Symposium on the Reproductive Physiology of Fish
  • Place of Presentation: オリョン（ ポルトガル）
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] The role of gonads for growth in teleosts (2014)
  • Author(s): Miura, T., Miura, C., Iwai,T and Butta, S.
  • Organizer: 10th International Symposium on the Reproductive Physiology of Fish
  • Place of Presentation: オリョン（ ポルトガル）
  • Year and Date: 2014-05-25 – 2014-05-30
• [Presentation] Positive effects of dietary housefly (Musca domestica) pupa for fish and mammal (2014)
  • Author(s): Ido, A., Ohta, T., Iwai, T., Kishida, T., Miura, C. and Miura, T.
  • Organizer: Insect to Feed the World
  • Place of Presentation: エーデ（ オランダ）
  • Year and Date: 2014-05-14 – 2014-05-17
• [Presentation] The benefits of using insects as fish and animal feed (2014)
  • Author(s): Miura, T., Ido, A., Ohta, T., Iwai, T., Kusano, K., Kobayashi, S., Kishida, T.and Miura, C.
  • Organizer: Insect to Feed the World
  • Place of Presentation: エーデ（ オランダ）
  • Year and Date: 2014-05-14 – 2014-05-17
• [Book] 「ホルモンから見た生命現象と進化」シリーズ 第3巻 成長・成熟・性決定－継－ (2016)
  • Author(s): 三浦 猛，三浦智恵美
  • Total Pages: 183
  • Publisher: 裳華房
• [Patent(Industrial Property Rights)] 成長又は摂餌促進剤、飼料、及び食品 (2018)
  • Inventor(s): 三浦猛,三浦智恵美,近藤 史崇,青木 良太
  • Industrial Property Rights Holder: 三浦猛,三浦智恵美,近藤 史崇,青木 良太
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2018-028338
  • Filing Date: 2018
• [Patent(Industrial Property Rights)] 組成物、及び組成物の製造方法 (2016)
  • Inventor(s): 三浦 猛, 三浦智恵美, 太田史, 橋爪篤史
  • Industrial Property Rights Holder: 三浦 猛, 三浦智恵美, 太田史, 橋爪篤史
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-113379
  • Filing Date: 2016-06-07