Accumulation mechanisms of tetrodotoxin on hybrid pufferfish and its parental species

• Project/Area Number: 26450287
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Aquatic life science
• Research Institution: Nagasaki University
• Principal Investigator: TAKATANI Tomohiro 長崎大学, 水産・環境科学総合研究科(水産), 教授 (90304972)
• Co-Investigator(Kenkyū-buntansha): 荒川 修 長崎大学, 水産・環境科学総合研究科(水産), 教授 (40232037)
• Co-Investigator(Renkei-kenkyūsha): SUZUKI Shigenori 独立行政法人水産総合研究センター, 増養殖研究所, 主任研究員 (60463105) ; MOCHIOKA Noritaka 九州大学, 大学院農学研究院, 准教授 (40212261)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: TTX / 交雑フグ / 食品衛生 / フグ毒 / 交雑起源種 / マフグ / トラフグ

Outline of Final Research Achievements

17 natural hybrid pufferfish were collected from Enshu-nada, and their parent species were identified by analysis of those mitochondrial DNA, and those anatomical distributions of toxicity were analyzed by LC/MS analysis. Two species were contained in all specimens, and they were identified as “matora” (Takifugu porphyreus x T. rublipes), and “torama” (T. rublipes x T. porphyreus), respectively. 15 specimens were toxic, toxicity level of the ovary and liver were high, and the skin showed weakly toxic. Matora specimens indicated higher toxicity than torama specimens. In the TTX administration experiment to artificial matora hybrid puffer, these mainly accumulated TTX in the skin. In the TTX administration experiment to artificial T. rublipes and T. porphyreus as a parental species, the TTX accumulation rate of both species rose by their growth. It is supposed that the toxin accumulation mechanisms are hereditary from female parental species

Research Products

• [Journal Article] Administration of tetrodotoxin protects artificially-raised juvenile tiger puffer Takifugu rubripes from predators. (2017)
  • Author(s): Sakakura, Y., Takatani, T., Nakayasu, J., Yamazaki, H., Sakiyama, K.
  • Journal Title: Fisheries Science Volume: 83 Issue: 2 Pages: 191-198
  • DOI: 10.1007/s12562-016-1046-0
  • NAID: 120006987423
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Transcriptome analysis of tetrodotoxin sensing and action of tetrodotoxin in central nervous system of tiger puffer Takifugu rubripes juveniles (2017)
  • Author(s): Okita, K., Tan, E., Satone, H., Kinoshita, S., Asakawa, S., Ojima, D., Yamazaki, H., Sakiyama, K., Takatani, T., Arakawa, O., Hagiwara, A., Sakakura, Y.
  • Journal Title: Fisheries Science Volume: 83 Issue: 3 Pages: 401-412
  • DOI: 10.1007/s12562-017-1079-z
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] 交雑フグの毒性 (2016)
  • Author(s): 高谷智裕、荒川 修、鈴木重則、望岡典隆
  • Journal Title: 日本水産学会誌 Volume: 82 Pages: 167-167
• [Journal Article] Growth and paralytic shellfish toxin production by the dinoflagellate Alexandrium catenella cultured under monochromatic light. (2016)
  • Author(s): Jiang, S., Iwashita, H., Arakawa, O., Takatani, T.
  • Journal Title: Aquaculture Science Volume: 64 Pages: 379-390
  • NAID: 130006258900
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Cytotoxic Glycosylated Fatty Acid Amides from a Stelletta sp. Marine Sponge (2015)
  • Author(s): V. Peddie, K. Takada, S. Okuda, Y. Ise, Y. Morii, N. Yamawaki, T. Takatani, O. Arakawa,⊥ S. Okada, and S. Matsunaga
  • Journal Title: Journal of Natural Products Volume: 78 Issue: 11 Pages: 2808-2813
  • DOI: 10.1021/acs.jnatprod.5b00795
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Larval pufferfish protected by maternal tetrodotoxin (2014)
  • Author(s): Itoi, S., Yoshikawa, S., Asahina, K., Suzuki, M., Ishizuka, K., Takimoto, N., Mitsuoka, R., Yokoyama, N., Detake, A., Takayanagi, C., Eguchi, M., Tatsuno, R., Kawane, M., Kokubo, S., Takanashi, S., Miura, A., Suitoh, K., Takatani, T., Arakawa, O., Sakakura, Y., Sugita, H.
  • Journal Title: Toxicon Volume: 78 Pages: 35-40
  • DOI: 10.1016/j.toxicon.2013.11.003
  • NAID: 120006986794
  • Peer Reviewed / Open Access
• [Presentation] 屋久島および石垣島産オウギガニ科カニ類の毒性プロファイル (2017)
  • Author(s): 宗宮史樹、高谷智裕、青島 隆、森井康宏、荒川 修
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学、東京都港区
  • Year and Date: 2017-03-26
• [Presentation] 窒素安定同位体を用いた紅藻マクリの培養 (2017)
  • Author(s): 姜 珊珊、桑野和可、Gregory N. Nishihara、浦田千里、下田隆介、高谷智裕、荒川 修
  • Organizer: 日本水産学会
  • Place of Presentation: 東京海洋大学、東京都港区
  • Year and Date: 2017-03-26
  • Int'l Joint Research
• [Presentation] Growth and Psp Production of the Toxic Dinoflagellate Alexandrium catenella Cultured under Monowavelength Light Irradiation. (2016)
  • Author(s): Jiang, S., Iwashita, H., Arakawa, O., Takatani, T.
  • Organizer: 7th World Fisheries Congress in Busan, Korea
  • Place of Presentation: Bexco国際展示場, Busan, Korea
  • Year and Date: 2016-05-23
  • Int'l Joint Research
• [Presentation] Providing pufferfish liver for human consumption (1) (2016)
  • Author(s): Taniguchi, K.，Takao，H., Onuki, K., Tatsuno, R., Sakakura, Y., Takatani, T., Arakawa, O., Noguchi, T.
  • Organizer: 7th World Fisheries Congress in Busan, Korea
  • Place of Presentation: Bexco国際展示場, Busan, Korea
  • Year and Date: 2016-05-23
  • Int'l Joint Research
• [Presentation] 交雑フグの毒性 (2015)
  • Author(s): 高谷智裕・荒川修・鈴木重則・望岡典隆
  • Organizer: 日本水産学会-ミニシンポジウム
  • Place of Presentation: 東北大学（宮城県仙台市）
  • Year and Date: 2015-09-22
• [Presentation] Transcriptome Analysis of Tetrodotoxin Sensing and Action of Tetrodotoxin in the Brain of Tiger Puffer Takifugu rubripes by Next-Generation Sequencing (2015)
  • Author(s): Kogen Okita, Hina Satone, Engkong Tan, Shigeharu Kinoshita, Shuichi Asakawa, Hideki Yamazaki, Kazutaka Sakiyama, Tomohiro Takatani, Osamu Arakawa, Atsushi Hagiwara, Yoshitaka Sakakura
  • Organizer: 145th American Fisheries Society Annual Meeting
  • Place of Presentation: Portland, Oregon, USA
  • Year and Date: 2015-08-16
  • Int'l Joint Research
• [Presentation] フグ毒を投与したトラフグ人工種苗の 嗅上皮および脳のトランスクリプトーム解析 (2014)
  • Author(s): 沖田光玄・佐藤根 妃奈・陳 盈光・木下滋晴・浅川修一・山崎英樹・崎山一孝・ 高谷智裕・荒川 修・阪倉良孝・萩原篤志
  • Organizer: 日本水産学会
  • Place of Presentation: 三重大学（三重県津市）
  • Year and Date: 2014-09-19 – 2014-09-22