Comparative analysis of the interaction of Rad51 recombinase and tumor suppressor BRCA2 aimed at drug development

• Project/Area Number: 26450400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: Morimatsu Masami 北海道大学, 獣医学研究科, 准教授 (70241370)
• Co-Investigator(Kenkyū-buntansha): 落合 和彦 日本獣医生命科学大学, 獣医学部, 准教授 (30550488)
• Co-Investigator(Renkei-kenkyūsha): ISHIGURO Toshina (OONUMA Toshina) 岩手大学, 農学部, 准教授 (60452695)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: DNA損傷修復 / 乳腺腫瘍 / 放射線感受性 / BRCA2 / Rad51

Outline of Final Research Achievements

Towards a better understanding of the molecular mechanisms of mammary tumor development in dogs, this study was focused on breast cancer tumor suppressor protein BRCA2 and its interacting recombinase Rad51 for an attempt to provide the basis of pure research leading to novel drug development and therapy for various mammals including dogs. Polymorphisms of canine BRCA2 affecting interaction with Rad51and reduced expression levels of BRCA2 in canine mammary tumors were found. Moreover, novel regulation mechanisms were shown for androgen receptor (AR) that is an BRCA2 interacting molecule.

Research Products

• [Int'l Joint Research] Kasetsart大学(タイ)
• [Journal Article] Tumor suppressor REIC/DKK-3 and co-chaperone SGTA: Their interaction and roles in the androgen sensitivity (2016)
  • Author(s): Ochiai K, Morimatsu M, Kato Y, Ishiguro-Oonuma T, Udagawa C, Rungsuriyawiboon O, Azakami D, Michishita M, Ariyoshi Y, Ueki H, Nasu Y, Kumon H, Watanabe M, Omi T.
  • Journal Title: Oncotarget Volume: 7 Issue: 3 Pages: 3283-3296
  • DOI: 10.18632/oncotarget.6488
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular cloning of canine co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA) and investigation of its ability to suppress androgen receptor signalling in androgen-independent prostate cancer. (2015)
  • Author(s): Kato, Y., Ochiai, K., Michishita, M., Azakami, D., Nakahira, R., Morimatsu, M., Ishiguro-Oonuma, T., Yoshikawa, Y., Kobayashi, M., Bonkobara, M., Kobayashi, M., Takahashi, K., Watanabe, M., and Omi, T.
  • Journal Title: The Vwterinary Journal Volume: 206 Issue: 2 Pages: 145-148
  • DOI: 10.1016/j.tvjl.2015.08.002
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Reduced canine BRCA2 expression levels in mammary gland tumors. (2015)
  • Author(s): Yoshikawa, Y., Morimatsu, M., Ochiai, K., Ishiguro-Oonuma, T., Wada, S., Orino, K. and Watanabe, K.
  • Journal Title: BMC Vet. Res. Volume: 11 Issue: 1 Pages: 159-166
  • DOI: 10.1186/s12917-015-0483-9
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] <b>Polymorphisms of canine BRCA2 BRC repeats affecting interaction with </b><b>RAD51 </b> (2015)
  • Author(s): Kazuhiko Ochiai, Toshina Ishiguro-Oonuma, Yasunaga Yoshikawa, Chihiro Udagawa, Yuiko Kato, Masami Watanabe, Makoto Bonkobara, Masami Morimatsu, Toshinori Omi
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 155-158
  • DOI: 10.2220/biomedres.36.155
  • NAID: 130005065275
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] <b>The role of IFN-γ in regulating Nfkbiz expression in epidermal </b><b>keratinocytes </b> (2015)
  • Author(s): Toshina Ishiguro-Oonuma, Kazuhiko Ochiai, Kazuyoshi Hashizume, Masami Morimatsu
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 103-107
  • DOI: 10.2220/biomedres.36.103
  • NAID: 130005065280
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] イヌ乳腺腫瘍サンプルにおけるBRCA2発現量の減少 (2015)
  • Author(s): 吉川泰永、森松正美、落合和彦、石黒-大沼俊名、和田成一、折野宏一
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学（青森県・十和田市）
  • Year and Date: 2015-09-07
• [Presentation] 癌抑制遺伝子BRCA2のイントロンにおける新規サイレンサー配列の同定と転写因子の特定 (2015)
  • Author(s): 角本俊介、吉川泰永、森松正美、落合和彦、石黒-大沼俊名、折野宏一
  • Organizer: 第158回日本獣医学会学術集会
  • Place of Presentation: 北里大学（青森県・十和田市）
  • Year and Date: 2015-09-07
• [Presentation] DNA相同組換えタンパク質RAD51の動物種間多型が機能に及ぼす影響の解析 (2014)
  • Author(s): 落合和彦、閑野大輝、宇田川智野、呰上大吾、大沼俊名、吉川泰永、森松正美、近江俊徳
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学（北海道・札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12