Molecular analysis of inhibition mechanisms of feline infectious peritonitis virus replication by cyclosporin.

• Project/Area Number: 26450413
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: TANAKA YOSHIKAZU 日本獣医生命科学大学, 獣医学部, 准教授 (50291159)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: ウイルス感染症 / ネコ伝染性腹膜炎 / ネココロナウイルス / サイクロスポリン / シクロフィリン / ウイルス複製制御 / 抗ウイルス薬 / ネコウイルス性感染症 / Pin1 / ウイルス複製 / 抗ウイルス剤

Outline of Final Research Achievements

Feline infectious peritonitis (FIP) is a viral infectious disease and lethal. We previously found that cyclosporin A (CsA) inhibits FIP virus (FIPV) replication in vitro and in vivo. We made cellular cyclophilin (Cyp) mutants which binds CsA less than with a wild type Cyp and transfected them into fcwf-4 cells. Consequently, these mutants suppressed FIPV replication less than those in transfected into the cells with the wild type Cyp. These findings show that CsA competitively binds the binding domain of Cyp with any cellular and/or viral factors.

Research Products

• [Journal Article] Feline coronavirus replication is affected by both cyclophilin A and cyclophilin B. (2017)
  • Author(s): Tanaka Y, Sato Y, Sasaki T.
  • Journal Title: Journal of General Virology Volume: 98 Issue: 2 Pages: 190-200
  • DOI: 10.1099/jgv.0.000663
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus. (2016)
  • Author(s): Tanaka Y, Amano A, Morisaki M, Sato Y, Sasaki T.
  • Journal Title: Antiviral Research Volume: 126 Pages: 1-7
  • DOI: 10.1016/j.antiviral.2015.11.013
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Treatment of a case of feline infectious peritonitis with cyclosporin A (2015)
  • Author(s): Tanaka Y, Sato Y, Takahashi D, Matsumoto H, Sasaki T.
  • Journal Title: Veterinary Record Case Reports Volume: 3 Issue: 1
  • DOI: 10.1136/vetreccr-2014-000134
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 gene (2015)
  • Author(s): Tanaka Y., Sasaki, T., Matsuda, R., Uematsu, Y., Yamaguchi, T.
  • Journal Title: BMC Veterinary Research Volume: 11 (1), art. No. 57 Issue: 1 Pages: 1-9
  • DOI: 10.1186/s12917-015-0372-2
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Induction of thioredoxin-1 in response to oxidative stress in dogs (2015)
  • Author(s): Munakata S, Tanaka Y, Nezu Y, Harada Y, Yogo T, Hara Y, Tian H, Matsuo Y, Tagawa M, Yodoi J.
  • Journal Title: Am J Vet Res. Volume: 76(6) Issue: 6 Pages: 554-60
  • DOI: 10.2460/ajvr.76.6.554
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Complete Genome Sequence of Methicillin-Resistant Staphylococcus schleiferi Strain TSCC54 of Canine Origin (2015)
  • Author(s): Sasaki T, Tsubakishita S, Kuwahara-Arai K, Matsuo M, Lu YJ, Tanaka Y,, Hiramatsu K.
  • Journal Title: Genome Announc. Volume: 29; 3(5) Issue: 5
  • DOI: 10.1128/genomea.01268-15
  • NAID: 120006311885
  • Peer Reviewed
• [Presentation] Cellular Peptidyl-Prolyl cis/trans Isomerase Pin1 Facilitates Replication of Feline Coronavirus (2016)
  • Author(s): Yoshikazu Tanaka
  • Organizer: American Society for Virology, 35th Annual meeting, 2016
  • Place of Presentation: Virginia Tech, Virginia, USA
  • Year and Date: 2016-06-18
  • Int'l Joint Research
• [Presentation] Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus (2016)
  • Author(s): Yoshikazu Tanaka, Yuka Sato, Takashi Sasaki
  • Organizer: 35th Annual Meeting, American Society for Virology
  • Place of Presentation: Virginia Tech, Virginia, USA
  • Year and Date: 2016-06-17
  • Int'l Joint Research
• [Presentation] FIPの基礎研究から臨床応用へのトランスレーショナルリサーチ (2015)
  • Author(s): 田中良和
  • Organizer: 日本獣医内科学アカデミー
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2015-02-20 – 2015-02-22
  • Invited
• [Presentation] サイクロスポリン製剤によるネコ伝染性腹膜炎治療効果 (2014)
  • Author(s): 田中良和
  • Organizer: 第157回 日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] FIP治療効果を遺伝子検査から読み取る ～基礎研究から臨床応用研究への展開の試み～ (2014)
  • Author(s): 田中良和
  • Organizer: 日本獣医臨床病理学会 2014年大会
  • Place of Presentation: 日本獣医生命科学大学
  • Year and Date: 2014-05-31 – 2014-06-01
  • Invited
• [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎の治療および予防するための医薬組成物およびその使用 (2014)
  • Inventor(s): 田中良和
  • Industrial Property Rights Holder: 学校法人 日本医科大学
  • Industrial Property Rights Type: 特許
  • Filing Date: 2014-05-31
  • Acquisition Date: 2014-09-12