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Molecular analysis of inhibition mechanisms of feline infectious peritonitis virus replication by cyclosporin.

Research Project

Project/Area Number 26450413
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Veterinary medical science
Research InstitutionNippon Veterinary and Life Science University

Principal Investigator

TANAKA YOSHIKAZU  日本獣医生命科学大学, 獣医学部, 准教授 (50291159)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywordsウイルス感染症 / ネコ伝染性腹膜炎 / ネココロナウイルス / サイクロスポリン / シクロフィリン / ウイルス複製制御 / 抗ウイルス薬 / ネコウイルス性感染症 / Pin1 / ウイルス複製 / 抗ウイルス剤
Outline of Final Research Achievements

Feline infectious peritonitis (FIP) is a viral infectious disease and lethal. We previously found that cyclosporin A (CsA) inhibits FIP virus (FIPV) replication in vitro and in vivo. We made cellular cyclophilin (Cyp) mutants which binds CsA less than with a wild type Cyp and transfected them into fcwf-4 cells. Consequently, these mutants suppressed FIPV replication less than those in transfected into the cells with the wild type Cyp. These findings show that CsA competitively binds the binding domain of Cyp with any cellular and/or viral factors.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (12 results)

All 2017 2016 2015 2014

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results,  Acknowledgement Compliant: 5 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Feline coronavirus replication is affected by both cyclophilin A and cyclophilin B.2017

    • Author(s)
      Tanaka Y, Sato Y, Sasaki T.
    • Journal Title

      Journal of General Virology

      Volume: 98 Issue: 2 Pages: 190-200

    • DOI

      10.1099/jgv.0.000663

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus.2016

    • Author(s)
      Tanaka Y, Amano A, Morisaki M, Sato Y, Sasaki T.
    • Journal Title

      Antiviral Research

      Volume: 126 Pages: 1-7

    • DOI

      10.1016/j.antiviral.2015.11.013

    • Related Report
      2016 Annual Research Report 2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Treatment of a case of feline infectious peritonitis with cyclosporin A2015

    • Author(s)
      Tanaka Y, Sato Y, Takahashi D, Matsumoto H, Sasaki T.
    • Journal Title

      Veterinary Record Case Reports

      Volume: 3 Issue: 1

    • DOI

      10.1136/vetreccr-2014-000134

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Molecular epidemiological study of feline coronavirus strains in Japan using RT-PCR targeting nsp14 gene2015

    • Author(s)
      Tanaka Y., Sasaki, T., Matsuda, R., Uematsu, Y., Yamaguchi, T.
    • Journal Title

      BMC Veterinary Research

      Volume: 11 (1), art. No. 57 Issue: 1 Pages: 1-9

    • DOI

      10.1186/s12917-015-0372-2

    • Related Report
      2015 Research-status Report 2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Induction of thioredoxin-1 in response to oxidative stress in dogs2015

    • Author(s)
      Munakata S, Tanaka Y, Nezu Y, Harada Y, Yogo T, Hara Y, Tian H, Matsuo Y, Tagawa M, Yodoi J.
    • Journal Title

      Am J Vet Res.

      Volume: 76(6) Issue: 6 Pages: 554-60

    • DOI

      10.2460/ajvr.76.6.554

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Complete Genome Sequence of Methicillin-Resistant Staphylococcus schleiferi Strain TSCC54 of Canine Origin2015

    • Author(s)
      Sasaki T, Tsubakishita S, Kuwahara-Arai K, Matsuo M, Lu YJ, Tanaka Y,, Hiramatsu K.
    • Journal Title

      Genome Announc.

      Volume: 29; 3(5) Issue: 5

    • DOI

      10.1128/genomea.01268-15

    • NAID

      120006311885

    • Related Report
      2015 Research-status Report
    • Peer Reviewed
  • [Presentation] Cellular Peptidyl-Prolyl cis/trans Isomerase Pin1 Facilitates Replication of Feline Coronavirus2016

    • Author(s)
      Yoshikazu Tanaka
    • Organizer
      American Society for Virology, 35th Annual meeting, 2016
    • Place of Presentation
      Virginia Tech, Virginia, USA
    • Year and Date
      2016-06-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cellular peptidyl-prolyl cis/trans isomerase Pin1 facilitates replication of feline coronavirus2016

    • Author(s)
      Yoshikazu Tanaka, Yuka Sato, Takashi Sasaki
    • Organizer
      35th Annual Meeting, American Society for Virology
    • Place of Presentation
      Virginia Tech, Virginia, USA
    • Year and Date
      2016-06-17
    • Related Report
      2015 Research-status Report
    • Int'l Joint Research
  • [Presentation] FIPの基礎研究から臨床応用へのトランスレーショナルリサーチ2015

    • Author(s)
      田中良和
    • Organizer
      日本獣医内科学アカデミー
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2015-02-20 – 2015-02-22
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] サイクロスポリン製剤によるネコ伝染性腹膜炎治療効果2014

    • Author(s)
      田中良和
    • Organizer
      第157回 日本獣医学会学術集会
    • Place of Presentation
      北海道大学
    • Year and Date
      2014-09-09 – 2014-09-12
    • Related Report
      2014 Research-status Report
  • [Presentation] FIP治療効果を遺伝子検査から読み取る ~基礎研究から臨床応用研究への展開の試み~2014

    • Author(s)
      田中良和
    • Organizer
      日本獣医臨床病理学会 2014年大会
    • Place of Presentation
      日本獣医生命科学大学
    • Year and Date
      2014-05-31 – 2014-06-01
    • Related Report
      2014 Research-status Report
    • Invited
  • [Patent(Industrial Property Rights)] ネコ伝染性腹膜炎の治療および予防するための医薬組成物およびその使用2014

    • Inventor(s)
      田中良和
    • Industrial Property Rights Holder
      学校法人 日本医科大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2014-05-31
    • Acquisition Date
      2014-09-12
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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