Development of a novel cryopreservation method of canine sperm

Research Project

Project/Area Number	26450428
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Veterinary medical science
Research Institution	Tottori University
Principal Investigator	Hishinuma Mitsugu 鳥取大学, 農学部, 教授 (30183578)
Research Collaborator	KIMURA Yusuke TAKASHIMA Rei SHIMIZU Hisako MORIYA Hikari ADACHI Naoki NISHIMURA Ryo
Project Period (FY)	2014-04-01 – 2017-03-31
Project Status	Completed (Fiscal Year 2016)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	犬 / 精子 / 低温保存 / 運動性 / 精子運動解析装置
Outline of Final Research Achievements	Sperm motility characteristics were examined by computer assisted sperm analysis system (SMAS) in fertile and infertile dogs. Motility parameters of frozen-thawed dog sperm were superior in the extender supplemented with disaccharide than monosaccharide. Type and binding mode of monosaccharide in disaccharide also affected motility parameters of frozen-thawed dog sperm. Cryopreservation of dog sperm affected morphometric findings of sperm head. In bovine semen, refrigeration affected sperm motility parameters.

Report

(4 results)