In-depth analysis of the SRBD1 gene region in canine glaucoma

• Project/Area Number: 26450436
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Veterinary medical science
• Research Institution: Azabu University
• Principal Investigator: Kanemaki Nobuyuki 麻布大学, 大学病院, 准教授 (40139530)
• Co-Investigator(Kenkyū-buntansha): 水木 信久 横浜市立大学, 医学研究科, 教授 (90336579)
• Co-Investigator(Renkei-kenkyūsha): MEGURO Akira 横浜市立大学, 医学研究科, 特任准教授 (60508802)
• Research Collaborator: ENDO Lisa ; NANBA Reiko
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 緑内障

Outline of Final Research Achievements

Glaucoma is a degenerative optic neuropathy that is associated with elevated intraocular pressure. We previously reported a significant association between the SRBD1 SNPs and glaucoma in humans and dogs. The purpose of this study was to clarify the contribution of SRBD1 in the development of glaucoma and we performed an in-depth of the SRBD1 gene region in several dog breeds.
In this study, we identified the intronic SNP in SRBD1 which exhibited the strongest association with glaucoma in both Shiba-Inus and Shih-Tzus. We also identified another intronic SNP showing a suggestive association with glaucoma in American Cocker Spaniels, Miniature Dachshunds, and Beagles. The functional analysis suggested that these SNPs affect gene expression of SRBD1.

Research Products

• [Journal Article] Fundus photography with a smartphone in indirect ophthalmoscopy in dogs and cats. (2017)
  • Author(s): Kanemaki N, Inaniwa M, Terakado K, Kawarai S, Ichikawa Y.
  • Journal Title: Veterinary ophthalmology Volume: 20 Issue: 3 Pages: 280-284
  • DOI: 10.1111/vop.12399
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evaluation of weekly bathing in allergic dogs with methicillin-resistant Staphylococcal colonization. (2016)
  • Author(s): Kawarai S, Fujimoto A, Nozawa G, Kanemaki N, Madarame H, Shida T, Kiuchi A.
  • Journal Title: The Japanese journal of veterinary research Volume: 64 Pages: 153-158
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Classification of Clinical Findings of Glaucoma in Dogs Presenting at the Ophthalmic Department of Azabu Veterinary Teaching Hospital (2015)
  • Author(s): 印牧信行、市川陽一朗、川原井晋平、落合秀治
  • Journal Title: Journal of the Japan Veterinary Medical Association Volume: 68 Issue: 1 Pages: 55-58
  • DOI: 10.12935/jvma.68.55
  • NAID: 130004888070
  • ISSN: 0446-6454, 2186-0211
  • Peer Reviewed
• [Presentation] Associations of single-nucleotide polymorphisms of SRBD1 gene 64 with glaucoma in Shiba-inu dog: A meta-analysis in six Japanese veterinary hospitals. (2015)
  • Author(s): Ota N, Kanemaki N, Tsujita H, Kobayashi Y, Abe M, Takimoto Y, Imayasu M, Meguro A, Mizuki N
  • Organizer: ACVO Annual meeting
  • Place of Presentation: Coeur d’Alene, ID, USA
  • Year and Date: 2015-10-07
  • Int'l Joint Research