Physiological function of phosphatidylinositol 3,5-bisphosphate in oligodendrocytes

• Project/Area Number: 26460059
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Biological pharmacy
• Research Institution: Akita University
• Principal Investigator: Takasuga Shunsuke 秋田大学, 医学(系)研究科(研究院), 助教 (90375262)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2015: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: イノシトールリン脂質

Outline of Final Research Achievements

To elucidate physiological functions of phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) in the central nervous system (CNS), we generated and analyzed CNS-specific type III phosphatidylinositol phosphate kinase (PIPKIII)-deficient mice. These mutant mice showed the severe demyelination in the brain, which suggest that PI(3,5)P2 regulates the myelination in CNS. Futhermore, the cell lineage-specific deletion of PIPKIII gene revealed that PIPKIII in oligodendrocytes but not neurons had essential roles for the myelination.

Research Products

• [Journal Article] Vps34 regulates myofibril proteostasis to prevent hypertrophic cardiomyopathy. (2017)
  • Author(s): Kimura H, Eguchi S, Sasaki J, Kuba K, Nakanishi H, Takasuga S, Yamazaki M, Goto A, Watanabe H, Itoh H, Imai Y, Suzuki A, Mizushima N, Sasaki T
  • Journal Title: JCI Insight Volume: 2 Issue: 1 Pages: 89462-89478
  • DOI: 10.1172/jci.insight.89462
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] The ML1Nx2 Phosphatidylinositol 3,5-Bisphosphate Probe Shows Poor Selectivity in Cells. (2015)
  • Author(s): Hammond GR, Takasuga S, Sasaki T, Balla T.
  • Journal Title: PLoS One Volume: 10 Issue: 10 Pages: e0139957-e0139957
  • DOI: 10.1371/journal.pone.0139957
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] INPP4B Is a PtdIns(3,4,5)P3 Phosphatase That Can Act as a Tumor Suppressor. (2015)
  • Author(s): Kofuji S, Kimura H, Nakanishi H, Nanjo H, Takasuga S, Liu H, Eguchi S, Nakamura R, Itoh R, Ueno N, Asanuma K, Huang M, Koizumi A, Habuchi T, Yamazaki M, Suzuki A, Sasaki J, Sasaki T.
  • Journal Title: Cancer Discov. Volume: 5 Pages: 365-375
  • Peer Reviewed
• [Journal Article] Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20. (2014)
  • Author(s): Segawa T, Hazeki K, Nigorikawa K, Morioka S, Guo Y, Takasuga S, Asanuma K, Hazeki O.
  • Journal Title: Biochem. J. Volume: 464 Issue: 3 Pages: 365-375
  • DOI: 10.1042/bj20140916
  • Peer Reviewed
• [Presentation] Muscle-specific type III phosphatidylinositol phosphate kinase (PIPKIII/PIKfyve) deficiency improves glucose tolerance and insulin resistance in mice. (2014)
  • Author(s): Shunsuke Takasuga, Takehiko Sasaki
  • Organizer: International symposium on "Homeostasis through development, life, and diseases."
  • Place of Presentation: Tojo Hall, Gunma Unicersity, Gunma
  • Year and Date: 2014-11-07
• [Book] 疾患モデルの作製と利用ー脂質代謝異常と関連疾患《下巻》 (2015)
  • Author(s): 高須賀俊輔、佐々木雄彦
  • Total Pages: 413
  • Publisher: 株式会社 エル・アイ・シー