| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
The mechanism of “false in vitro elevation of uric acid level” is clarified using mice with highly active hypoxanthine phosphoribosyl transferase (Hprt) of B6-ChrXC-MSM, a consomic mouse strain with the chromosome portion of Mus musclus morocinus in the Hprt gene site, or urate oxidase gene (Uox) targeted mice (Uox-KO).
Uric acid level of B6-ChrXC-MSM blood tended to be lower than those of control blood. Those data were significantly lower than that of control. Moreover, uric acid level of Uox-KO blood, which was about 10 times higher than that of control, did not elevated after incubation in the test tube. Those data were also significantly lower than that of control. In conclusion, “false in vitro elevation of uric acid level” seems to be caused by the low levels of erythrocyte HPRT activity and high plasma uric acid level of laboratory mice.
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