Elucidation of the machinery for the biosynthesis of sulfur-containing bioactive compounds in medicinal plants

• Project/Area Number: 26460118
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Chiba University
• Principal Investigator: Yoshimoto Naoko 千葉大学, 大学院薬学研究院, 講師 (10415333)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 植物 / 生合成 / 含硫黄成分 / トランスクリプトーム / 天然薬用資源 / システインスルホキシド / 遺伝子 / 薬学 / 硫黄代謝物 / 含硫黄薬理成分 / ディープトランスクリプトーム / ネギ属 / ツルバギア属 / ニンニクカズラ属

Outline of Final Research Achievements

S-alk(en)ylcysteine sulfoxides are health-beneficial sulfur-containing compounds biosynthesized in plants that belong to genera Allium, Tulbaghia, and Mansoa. In this study, we performed deep-transcriptome and metabolite analyses of Allium, Tulbaghia, and Mansoa plants, and identified some enzymatic genes that are involved in the biosynthesis of S-alk(en)ylcysteine sulfoxides.

Research Products

• [Journal Article] Measurement of uptake and root-to-shoot distribution of sulfate in Arabidopsis seedlings (2016)
  • Author(s): Naoko Yoshimoto, Tatsuhiko Kataoka, Akiko Maruyama-Nakashita, Hideki Takahashi
  • Journal Title: Bio-protocol Volume: 6
  • NAID: 120007008029
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Identification of a flavin-containing S-oxygenating monooxygenase involved in alliin biosynthesis in garlic (2015)
  • Author(s): Naoko Yoshimoto, Misato Onuma, Shinya Mizuno, Yuka Sugino, Ryo Nakabayashi, Shinsuke Imai, Tadamitsu Tsuneyoshi, Shin-ichiro Sumi, Kazuki Saito
  • Journal Title: The Plant Journal Volume: 83 Issue: 6 Pages: 941-951
  • DOI: 10.1111/tpj.12954
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Garlic γ-glutamyl transpeptidases that catalyze deglutamylation of biosynthetic intermediate of alliin. (2015)
  • Author(s): Naoko Yoshimoto, Ayami Yabe, Yuka Sugino, Soichiro Murakami, Niti Sai-ngam, Shin-ichiro Sumi, Tadamitsu Tsuneyoshi and Kazuki Saito
  • Journal Title: Front. Plant Sci. Volume: 5 Pages: 758-758
  • DOI: 10.3389/fpls.2014.00758
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] ディープトランスクリプトーム解析によるニンニクの機能性含硫黄成分生合成に関わる酵素遺伝子群の探索 (2017)
  • Author(s): 吉本尚子，森直子，佐野彩夏，石井梨紗子，浅野雅代，鈴木秀幸，小寺幸広，恒吉唯充，斉藤和季
  • Organizer: 日本薬学会第137年会
  • Place of Presentation: 仙台
  • Year and Date: 2017-03-24
• [Presentation] トランスクリプトームマイニングによるニンニクの硫黄二次代謝に関与する遺伝子群の解析 (2017)
  • Author(s): 吉本尚子，森直子，佐野彩夏，石井梨紗子，浅野雅代，鈴木秀幸，小寺幸広，恒吉唯充，斉藤和季
  • Organizer: 第58回日本植物生理学会年会
  • Place of Presentation: 鹿児島
  • Year and Date: 2017-03-16
• [Presentation] ゲノムマイニングに基づくニンニクの含硫黄機能性成分の生合成に関与する酵素遺伝子群の同定 (2016)
  • Author(s): 吉本尚子，森直子，佐野彩夏，石井梨紗子，浅野雅代，小沼美沙都，矢部綾美，杉野由佳，鈴木秀幸，小寺幸広，恒吉唯充，斉藤和季
  • Organizer: 第21回天然薬物の開発と応用シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-10-27
• [Presentation] 機能性含硫黄成分の生合成に関する分子生物学的研究 (2016)
  • Author(s): 吉本尚子
  • Organizer: 日本生薬学会第63回年会
  • Place of Presentation: 富山
  • Year and Date: 2016-09-24
  • Invited
• [Presentation] Transcriptome analysis of garlic toward the identification of genes involved in the biosynthesis of sulfur-containing compounds (2016)
  • Author(s): Naoko Yoshimoto, Naoko Mori, Ayaka Sano, Risako Ishii, Masayo Asano, Misato Onuma, Ayami Yabe, Yuka Sugino, Hideyuki Suzuki, Yukihiro Kodera, Tadamitsu Tsuneyoshi, Kazuki Saito
  • Organizer: 5th Sulphyton Workshop
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-09-14
  • Int'l Joint Research
• [Presentation] Identification of two genes encoding S-oxygenases involved in the biosynthesis of S-alk(en)ylcysteine sulfoxides in garlic (2016)
  • Author(s): Naoko Mori, Naoko Yoshimoto, Misato Onuma, Shinya Mizuno, Hideyuki Suzuki, Yukihiro Kodera, Ryo Nakabayashi, Tadamitsu Tsuneyoshi, Kazuki Saito
  • Organizer: 5th Sulphyton Workshop
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2016-09-14
  • Int'l Joint Research
• [Presentation] ニンニクの含硫黄化合物生合成に関わる二つのS-酸化酵素遺伝子の機能解析 (2016)
  • Author(s): 森直子，吉本尚子，小沼美沙都，鈴木秀幸，小寺幸広，恒吉唯充，斉藤和季
  • Organizer: 第34回日本植物細胞分子生物学会大会
  • Place of Presentation: 上田
  • Year and Date: 2016-09-01
• [Presentation] ニンニクの含硫黄薬理成分生合成に関わるS-酸化酵素遺伝子のホモログ遺伝子AsFMO2の単離 (2016)
  • Author(s): 森直子、吉本尚子、小沼美沙都、鈴木秀幸、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 日本薬学会第136年会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] ニンニクの含硫黄薬理成分アリインの生合成機構の解析と酵素遺伝子の同定 (2016)
  • Author(s): 吉本尚子
  • Organizer: 第４回植物二次代謝フロンティア研究会例会
  • Place of Presentation: 花巻
  • Year and Date: 2016-03-21
  • Invited
• [Presentation] ニンニクにおけるシステインスルホキシド誘導体の生合成に関与するS-酸化酵素遺伝子の新規ホモログAsFMO2の同定 (2016)
  • Author(s): 森直子、吉本尚子、小沼美沙都、鈴木秀幸、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 第57回日本植物生理学会年会
  • Place of Presentation: 盛岡
  • Year and Date: 2016-03-18
• [Presentation] ゲノムマイニングによるニンニクの含硫黄二次代謝産物生合成遺伝子の同定 (2016)
  • Author(s): 吉本尚子
  • Organizer: 千葉大学戦略的重点研究強化プログラム「ファイトケミカル植物分子科学」プロジェクト第１回公開シンポジウム
  • Place of Presentation: 千葉
  • Year and Date: 2016-03-16
  • Invited
• [Presentation] 植物での硫黄の輸送、代謝と含硫黄成分の生合成に関する分子生物学的研究 (2015)
  • Author(s): 吉本尚子
  • Organizer: 第６回食品薬学シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2015-10-30
  • Invited
• [Presentation] ニンニクにおける含硫二次代謝物生合成酵素遺伝子群の器官特異的発現と機能分担 (2015)
  • Author(s): 吉本尚子、杉野由佳、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 日本生薬学会第62回年会
  • Place of Presentation: 岐阜
  • Year and Date: 2015-09-11
• [Presentation] Biosynthesis of sulfur-containing secondary metabolites in garlic (2015)
  • Author(s): Naoko Yoshimoto, Kazuki Saito
  • Organizer: 10th Jubilee Plant Sulfur Workshop
  • Place of Presentation: Goslar, Germany
  • Year and Date: 2015-09-01
  • Int'l Joint Research / Invited
• [Presentation] ニンニクのアリイン生合成に関与する新規S-酸化酵素遺伝子の単離 (2015)
  • Author(s): 森直子、吉本尚子、小沼美沙都、鈴木秀幸、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 第33回日本植物細胞分子生物学会大会
  • Place of Presentation: 東京
  • Year and Date: 2015-08-10
• [Presentation] ニンニクにおける含硫二次代謝物生合成酵素群の器官特異的発現の解析 (2015)
  • Author(s): 吉本尚子、杉野由佳、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 日本薬学会第135年会
  • Place of Presentation: 神戸
  • Year and Date: 2015-03-25 – 2015-03-28
• [Presentation] ニンニクにおける含硫二次代謝物生合成酵素群の発現部位の解析 (2015)
  • Author(s): 吉本尚子、杉野由佳、小寺幸広、恒吉唯充、斉藤和季
  • Organizer: 第56回日本植物生理学会年会
  • Place of Presentation: 東京
  • Year and Date: 2015-03-16 – 2015-03-18
• [Presentation] ニンニクの含硫二次代謝物アリインの生合成に関わるS酸化酵素遺伝子AsFMO1の機能解析 (2014)
  • Author(s): 小沼美沙都、吉本尚子、水野新也、中林亮、今井真介、角愼一郎、恒吉唯充、斉藤和
  • Organizer: 日本生薬学会第61回年会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-13 – 2014-09-14
• [Presentation] Functional characterization of de-glutamylating and S-oxygenating enzymes for the alliin biosynthesis in garlic (2014)
  • Author(s): Naoko Yoshimoto, Ayami Yabe, Misato Onuma, Yuka Sugino, Ryo Nakabayashi, Masae Ueyama, Yasuhiro Kamata, Shinsuke Imai, Shin-ichiro Sumi, Tadamitsu Tsuneyoshi, Kazuki Saito
  • Organizer: 9th International Workshop on Sulfur Metabolism in Plants
  • Place of Presentation: Freiburg, Germany
  • Year and Date: 2014-04-14 – 2014-04-17
• [Book] Biosynthesis of S-alk(en)yl-L-cysteine sulfoxides in Allium - Retro perspective. in "Sulfur Metabolism in Higher Plants - Fundamental, Environmental and Agricultural Aspects" (2017)
  • Author(s): Naoko Yoshimoto, Kazuki Saito
  • Publisher: Springer
• [Book] Sulfur Metabolism in Plants: Molecular Physiology and Ecophysiology (2015)
  • Author(s): Naoko Yoshimoto, Kazuki Saito
  • Publisher: Springer
• [Remarks] 千葉大学 特色ある研究活動の成果「ニンニクの薬用成分の生産の鍵となる遺伝子の発見」
  • URL: http://www.chiba-u.ac.jp/research/coe_gp/result/result34.html