Suppressive mechanism of pancreatic beta cell failure in obese/type 2 diabetes model mice by mulberry leaf intake

• Project/Area Number: 26460121
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Natural medicines
• Research Institution: Kyoto Institute of Technology
• Principal Investigator: Kamei Kaeko 京都工芸繊維大学, 分子化学系, 教授 (00214544)
• Co-Investigator(Kenkyū-buntansha): 横出 正之 京都大学, 医学研究科, 教授 (20252447)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 糖尿病 / 耐糖能異常 / 膵β細胞 / 桑葉 / インスリン抵抗性 / アポトーシス / インスリン産生細胞 / 耐糖能

Outline of Final Research Achievements

In this study, we assumed the improving mechanism of impaired glucose tolerance by mulberry leaf intake from the viewpoint of the suppressive effects on pancreatic beta cell failure. We analyzed the effect of mulberry leaves intake on the proliferation of pancreatic beta cells at the compensation stage, in which pancreatic beta cell volume and insulin secretion temporarily increase, and the effect on the pancreatic beta cell death at the incompensation stage, using obese/type 2 diabetes model mice (db/db mice). We also studied the effect of mulberry leaf extract on cultured beta cells.
In db/db mice, the oral administration of mulberry leaf reduced ER stress and oxidative stress in pancreatic beta cells and suppressed cell death at the incompensation stage. We found cell proliferation promoting activity on cultured beta cells in mulberry leaves extract. Thus, we could clarify a part of glucose tolerance improvement mechanism by mulberry leaf intake.

Research Products

• [Int'l Joint Research] Cantho University/University of Science, VNU-HCM/Hanoi Medical University(ベトナム)
• [Journal Article] Function of Lipid Storage Droplet 1 (Lsd1) in Wing Development of Drosophila melanogaster. (2016)
  • Author(s): Tran Thanh Men , Tran Duy Binh , Masamitsu Yamaguchi , Nguyen Tien Huy and Kaeko Kamei
  • Journal Title: Int. J. Mol. Sci. Volume: 17(5) Issue: 5 Pages: 648-648
  • DOI: 10.3390/ijms17050648
  • Peer Reviewed / Open Access
• [Journal Article] A Drosophila Model for Screening Antiobesity Agents. (2016)
  • Author(s): Tran Thanh Men, Duong Ngoc Van Thanh, Masamitsu Yamaguchi, Takayoshi Suzuki, Gen Hattori, Masayuki Arii, Nguyen Tien Huy, and Kaeko Kamei.
  • Journal Title: BioMed Research International Volume: 2016 Pages: 6293163-6293163
  • DOI: 10.1155/2016/6293163
  • Peer Reviewed / Open Access
• [Journal Article] Effect of Galangal (Alpinia galanga Linn.) Extract on the Growth Rate and Resistance to Vibrio harveyi and White Spot Diseases in Pacific White Shrimp (Litopenaeus vannamei). (2015)
  • Author(s): Tidaporn Chaweepack, Chutima Khomvilai, Surachart Chaweepack and Kaeko Kamei
  • Journal Title: J. Agricultural Science Volume: 7 Issue: 9 Pages: 117-128
  • DOI: 10.5539/jas.v7n9p117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Potential of Galangal (Alpinia galang Linn.) Extract against the Pathogens that Cause White Feces Syndrome and Acute Hepatopancreatic Necrosis Disease (AHPND) in Pacific White Shrimp (Litopenaeus vannamei). (2015)
  • Author(s): Tidaporn Chaweepack, Boonyee Muenthaisong, Surachart Chaweepack and Kaeko Kamei
  • Journal Title: International Journal of Biology Volume: 7 Issue: 3 Pages: 1-10
  • DOI: 10.5539/ijb.v7n3p8
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] AMAP1 as a negative-feedback regulator of nuclear factor-κB under inflammatory conditions. (2014)
  • Author(s): Tien, D.N., Kishihata, M., Yoshikawa, A., Hashimoto, A., Sabe, H., Nishi, E., Kamei, K., Arai, H., Kita, T., Kimura, T., Yokode, M. and Ashida, N.
  • Journal Title: Sci Rep. Volume: 4 Issue: 1 Pages: 5094-5094
  • DOI: 10.1038/srep05094
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Aspirin augments the expression of Adenomatous Polyposis Coli protein by suppression of IKKβ (2014)
  • Author(s): Noboru Ashida, Masako Kishihata, Dat Nguyen Tien, Kaeko Kamei, Takeshi Kimura, Masayuki Yokode
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 446 Issue: 2 Pages: 460-464
  • DOI: 10.1016/j.bbrc.2014.02.134
  • Peer Reviewed
• [Presentation] Function of lipid storage droplet 1 (Lsd1) in wing development of Drosophila melanogaster (2016)
  • Author(s): Tran Men Than, Duy Binh Tran, 劉冠辰, 山口政光, Tien Huy Nguyen, 亀井加恵子
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] 糖尿病モデルマウスにおける桑葉の経口投与による耐糖能改善機構 (2015)
  • Author(s): 柚木 英里, 岡田 峻明, 南 学, 村山 敏典, 横出 正之, 亀井 加恵子
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] 桑葉抽出物による膵β細胞の細胞保護効果 (2015)
  • Author(s): 江角 拓麻, 岡田 峻明, 田村 友嗣, 南 学, 村山 敏典, 横出 正之, 亀井 加恵子
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] ショウジョウバエにおける脂質代謝関連遺伝子の機能 (2015)
  • Author(s): 劉 冠辰, Men T. Tran, Thi M. Dao, 山口 政光, 亀井 加恵子
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Role of lipid storage droplet-associated gene in Drosophila wing development (2015)
  • Author(s): Men T. Tran, Masamitsu Yamaguchi, Kaeko Kamei
  • Organizer: 第88回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2015-12-01
• [Presentation] Roles of IKKβ in fibrosis (2014)
  • Author(s): Dat Nguyen-tien, Masako Kishihata, Megumi Nishio, Kaeko Kamei, Kenji Kabashima, Yoshiki Miyachi, Takeshi Kimura, Masayuki Yokode, Noboru Ashida
  • Organizer: 第３７回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-11-25 – 2014-11-27
• [Remarks] 生体分子機能化学研究室
  • URL: http://biomole-func-chem-turtle.jimdo.com