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Establishment of a SPR system to screen for plant-based seed compounds for drugs against lifestyle diseases

Research Project

Project/Area Number 26460131
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Natural medicines
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

KURODA Minpei  東京薬科大学, 薬学部, 准教授 (80266890)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2016: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2015: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywords表面プラズモン共鳴 / 抗生活習慣病薬 / スクリーニング / aldose reductase / 植物エキス / xanthine oxidase / PPAR-γ / Agrimonia eupatoria / flavoniod配糖体 / Achillea millefolium / キナ酸誘導体
Outline of Final Research Achievements

Using a SPR system, we analyzed the interaction between aldose reductase (AR), which plays a role in diabetic complications, placed on the chip and a plant extract with AR inhibitory activity as determined by a conventional method. The resulting sensorgram showed no interaction. Then, we prepared four different plant extracts with AR inhibitory activity and isolated 49 compounds, including four new compounds, from the extracts. Among the compounds, dicaffeoylquinic acid (DCQA) derivatives exhibited strong inhibitory activity toward AR, and the activity decreased when the carboxylic acid of the DCQA was converted to a methyl ester. Our findings also revealed that the activity of the DCQA was affected by the binding site and number of caffeoyl groups.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (8 results)

All 2017 2016 2015 2014

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (6 results)

  • [Journal Article] Chemical Compounds from the Leaves of Verbascum thapsus and Their Xanthine Oxidase Inhibitory Activity2017

    • Author(s)
      Minpei Kuroda, Katsura Iwabuchi, Saori Usui, Nanami Akiyama, Yoshihiro Mimaki
    • Journal Title

      The Japanese Journal of Pharmacognosy

      Volume: 71 Pages: 49-50

    • NAID

      130007437585

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemical Constituents of the Leaves of Tussilago farfara and Their Aldose Reductase Inhibitory Activity2016

    • Author(s)
      Minpei Kuroda, Takumi Ohshima, Chihiro Kan, Yoshihiro Mimaki
    • Journal Title

      Natural Product Communications

      Volume: 11 Pages: 1661-1664

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed
  • [Presentation] カバノキ科 Betula alba 樹皮の化学成分と aldose reductase 阻害活性2016

    • Author(s)
      秋山 七海,黒田 明平,三巻 祥浩
    • Organizer
      日本生薬学会 第63回年会
    • Place of Presentation
      富山国際会議場
    • Year and Date
      2016-09-24
    • Related Report
      2016 Annual Research Report
  • [Presentation] 天然由来のα-glucosidase 阻害活性物質の探索に関する研究2015

    • Author(s)
      熊谷 侑紀,黒田 明平,三巻 祥浩
    • Organizer
      第59回 日本薬学会 関東支部大会
    • Place of Presentation
      日本大学
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] マメ科 Trifolium pratense 種子および T. repens 種子の化学成分と生物活性2015

    • Author(s)
      石井麻里奈,菅 千紘,熊谷 侑紀,泉澤 彩美,黒田 明平,三巻 祥浩
    • Organizer
      第59回 日本薬学会 関東支部大会
    • Place of Presentation
      日本大学
    • Year and Date
      2015-09-12
    • Related Report
      2015 Research-status Report
  • [Presentation] キク科 Tussilago farfara 葉およびバラ科 Agrimonia eupatoria 全草の化学成分と aldose reductase 阻害活性2015

    • Author(s)
      菅 千紘,大島 匠,黒田 明平,三巻 祥浩
    • Organizer
      日本生薬学会 第62回年会
    • Place of Presentation
      長良川国際会議場
    • Year and Date
      2015-09-11
    • Related Report
      2015 Research-status Report
  • [Presentation] キク科 Achillea millefolium 地上部の化学成分と aldose reductase 阻害活性に関する研究2014

    • Author(s)
      成田 圭,大島 匠,黒田 明平,三巻 祥浩
    • Organizer
      日本生薬学会 第61回年会
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-09-13 – 2014-09-14
    • Related Report
      2014 Research-status Report
  • [Presentation] ユリ科植物の化学成分(82)Eremurus ruiteri の化学成分について(3)2014

    • Author(s)
      井口 巴樹,黒田 明平,三巻 祥浩
    • Organizer
      日本生薬学会 第61回年会
    • Place of Presentation
      福岡大学
    • Year and Date
      2014-09-13 – 2014-09-14
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2018-03-22  

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