| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
In this study, we investigated the changes in gene expression levels in various tissues of methylmercury (MeHg)-treated mice and found that MeHg induced brain-specific expression of secretoglobin SCGB3A1. We also found that knockdown of SCGB3A1 increased the toxicity of MeHg in mouse neural progenitor C17.2 cells. Moreover, addition of recombinant SCGB3A1 to culture medium decreased the toxicity of MeHg in C17.2 cells, suggesting that extracellular SCGB3A1 plays a key role in the defense mechanism against toxicity of MeHg. Furthermore, NF-κB and TCF3, transcription factors, were involved in MeHg-induced SCGB3A1 expression.
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