Development of sustained-release formulations for cedar pollen-specific desensitization therapy

• Project/Area Number: 26460226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: University of Shizuoka
• Principal Investigator: Iwao Yasunori 静岡県立大学, 薬学部, 准教授 (30433022)
• Co-Investigator(Kenkyū-buntansha): 野口 修治 東邦大学, 薬学部, 教授 (60237823) ; 板井 茂 静岡県立大学, 薬学部, 教授 (80453059)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 減感作 / 製剤学 / マイクロ・ナノデバイス / アレルギー・ぜんそく / スギ花粉 / 減感作療法

Outline of Final Research Achievements

In this study, to develop a sustained-release formulation for cedar pollen-specific desensitization therapy, we determined the necessary amount or released rate of cryptomeria japonica pollen 1 (CryJ1), one of main allergen antigens of Japan cedar pollen, and we tried to prepare sustained-release formulation with long-term stability of CryJ1. As a result, the CryJ1 released rate to lead to immune tolerances was found to be 132 ng/day, and we succeeded in preparing PLAPEG9604H microspheres containing CryJ1 showing the sustained release at zero order manner. In addition, any structural changes of the cryj1 after long-term incubation were not found to influence the desensitization therapy.

Research Products

• [Journal Article] Nobiletin: a citrus flavonoid displaying potent physiological activity. (2016)
  • Author(s): Noguchi, S., Atsumi, H., Iwao, Y., Kan, T. & Itai, S.
  • Journal Title: Acta Crystallographica Volume: C72 Issue: 2 Pages: 193-198
  • DOI: 10.1107/s2053229616000577
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Investigation of Physicochemical Drug Properties to Prepare Fine Globular Granules Composed of Only Drug Substance in Fluidized Bed Rotor Granulation (2015)
  • Author(s): Mise R, Iwao Y, Kimura S, Osugi Y, Noguchi S, Itai S.
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 63 Issue: 12 Pages: 1070-1075
  • DOI: 10.1248/cpb.c15-00491
  • NAID: 130005112212
  • ISSN: 0009-2363, 1347-5223
• [Journal Article] Preparation and Evaluation of Newly Developed Chitosan Salt Coating Dispersions for Colon Delivery without Requiring Overcoating (2015)
  • Author(s): Yamada K, Iwao Y, Bani-Jaber A, Noguchi S, Itai S.
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 63 Issue: 10 Pages: 799-806
  • DOI: 10.1248/cpb.c15-00308
  • NAID: 130005100925
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Effect of Alkyl Chain Length and Unsaturation of the Phospholipid on the Physicochemical Properties of Lipid Nanoparticles (2015)
  • Author(s): Effect of Alkyl Chain Length and Unsaturation of the Phospholipid on the Physicochemical Properties of Lipid Nanoparticles.
  • Journal Title: Chemical and Pharmaceutical Bulletin Volume: 63 Issue: 9 Pages: 731-736
  • DOI: 10.1248/cpb.c15-00077
  • NAID: 130005096027
  • ISSN: 0009-2363, 1347-5223
  • Peer Reviewed
• [Journal Article] Structural investigation of spherical hollow excipient Mannit Q by X-ray microtomography. (2015)
  • Author(s): Kajihara, R., Noguchi, S., Iwao, Y., Yasuda, Y., Segawa, M. & Itai, S.
  • Journal Title: International Journal of Pharmaceutics Volume: 495 Issue: 1 Pages: 204-217
  • DOI: 10.1016/j.ijpharm.2015.08.097
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] Effect of alkyl chain length of phospholipids on the physicochemical properties and stability of lipid nanoparticles (2015)
  • Author(s): Yuka Funakoshi, Yasunori Iwao, Shuji Noguchi, and Shigeru Itai.
  • Organizer: American Association of Pharmaceutical Scientists Annual Meeting and Exposition
  • Place of Presentation: Orlando, USA
  • Year and Date: 2015-10-27
  • Int'l Joint Research
• [Presentation] マイクロウェーブによる口腔内崩壊茶錠の設計と評価 (2015)
  • Author(s): 田中大智、岩尾康範、石田均司、野口修治、板井茂
  • Organizer: 日本薬剤学会第30年会
  • Place of Presentation: 長崎
  • Year and Date: 2015-05-21
• [Presentation] 敗血症治療を目指したフラボン C 配糖体内封脂質ナノ粒子の調製と評価 (2015)
  • Author(s): 土村彩花、岩尾康範、石田均司、舟越由香、冨口依津美、野口修治、板井茂
  • Organizer: 日本薬剤学会第30年会
  • Place of Presentation: 長崎
  • Year and Date: 2015-05-21
• [Remarks] 静岡県立大学薬学部創剤工学分野
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/pharmeng/index.html