Morphological and Molecular Cell Biological Study on an Intermediate filament protein, synemin.

• Project/Area Number: 26460268
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: General anatomy (including histology/embryology)
• Research Institution: Gunma University
• Principal Investigator: Yorifuji Hiroshi 群馬大学, 大学院医学系研究科, 教授 (00158544)
• Co-Investigator(Kenkyū-buntansha): 村上 徹 群馬大学, 大学院医学系研究科, 准教授 (10239494) ; 多鹿 友喜 群馬大学, 大学院医学系研究科, 講師 (90400738) ; 上野 仁之 群馬大学, 大学院医学系研究科, 助教 (30586251)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: シネミン / 中間径フィラメント / 骨格筋 / 肝臓 / 線維化 / 肝線維化 / 細胞骨格タンパク

Outline of Final Research Achievements

To reveal the functions of intermediate filament protein, synemin, we studied skeletal muscles and liver. In skeletal muscles, the interaction between actinin 3 (ACTN3) and synemin was studied using immunoprecipitation and fluorescence microscope. Co-precipitation and co-localization of these proteins were found. Whereas mice lacking ACTN3 showed improvement of endurance, those lacking synemin showed its decline. We are now studying the mechanisms of the latter. In the liver, synemin is expressed in stellate cells. In experimental fibrosis models of the liver, synemin knockout mice showed the reduction of fibrosis compared with that of the wild type animals. This might lead to the prevention and/or treatment of fibrosis in human liver.

Research Products

• [Journal Article] Organization of organelles and VAMP-associated vesicular transport systems in differentiating skeletal muscle cells (2015)
  • Author(s): Tajika, Y., Takahashi, M., Ueno, H., Murakami, T., & Yorifuji, H
  • Journal Title: Anatomical Science International Volume: 90 Issue: 1 Pages: 33-39
  • DOI: 10.1007/s12565-014-0266-6
  • Peer Reviewed
• [Journal Article] A cadaveric study of the cervical nerve roots and spinal segments. (2015)
  • Author(s): Kobayashi R, Iizuka H, Nishinome, M, Iizuka, Y, Yorifuji, H, Takagishi, K
  • Journal Title: European Spine Journal Volume: 24 Issue: 12 Pages: 2828-2831
  • DOI: 10.1007/s00586-015-4070-3
  • Peer Reviewed
• [Journal Article] Analysis of human cytomegalovirus replication in primary cultured human corneal endothelial cells. (2015)
  • Author(s): Hosogai, M, Shima, N, Nakatani, Y, Inoue, T, Iso, T, Yokoo, H, Yorifuji, H, Akiyama, H, Kishi, S, Isomura, H.
  • Journal Title: British Journal of Ophthalmology Volume: 99 Issue: 11 Pages: 1583-1590
  • DOI: 10.1136/bjophthalmol-2014-306486
  • Peer Reviewed
• [Journal Article] Filamentous structures in skeletal muscle: anchors for the subsarcolemmal space (2014)
  • Author(s): Astrid Feinisa Khairani, Yuki Tajika, Maiko Takahashi, Hitoshi Ueno, Tohru Murakami, Arifin Soenggono & Hiroshi Yorifuji
  • Journal Title: Medical Molecular Morphology Volume: e-published Issue: 1 Pages: 1-12
  • DOI: 10.1007/s00795-014-0070-3
  • Peer Reviewed
• [Presentation] 四塩化炭素による急性肝炎の誘発における中間径フィラメントsynemin欠損の影響 (2017)
  • Author(s): 上野仁之、多鹿友喜、村上徹、池澤麻衣子、依藤宏
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] 胎生期マウスの2Dおよび3D形態解析 (2017)
  • Author(s): 多鹿友喜、村上徹、池澤麻衣子、上野仁之、依藤宏
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] 中間径フィラメントSyneminの欠損による骨格筋の変化 (2016)
  • Author(s): 上野仁之、佐藤真人、池澤麻衣子、多鹿友喜、村上 徹、依藤 宏
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市）
  • Year and Date: 2016-03-28
• [Presentation] 新生仔マウスの全身3D形態解析法 (2016)
  • Author(s): 多鹿友喜、村上 徹、池澤麻衣子、上野仁之、依藤 宏
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市)
  • Year and Date: 2016-03-28
• [Presentation] Visible Fish Danio rerio (2016)
  • Author(s): 村上 徹、多鹿友喜、上野仁之、池澤麻衣子、依藤 宏
  • Organizer: 第121回日本解剖学会総会・全国学術集会
  • Place of Presentation: ビッグパレットふくしま（福島県郡山市)
  • Year and Date: 2016-03-28
• [Presentation] Serial block-face imaging system for both 3D morphology and section histology. (2015)
  • Author(s): Yuki Tajika, Tohru Murakami, Maiko Takahashi, Hitoshi Ueno, Hiroshi Yorifuji
  • Organizer: 2015 American Society For Cell Biology Meeting
  • Place of Presentation: アメリカ サンジエゴ
  • Year and Date: 2015-12-12
• [Presentation] Molecular genetics of type IV intermediate filament, synemin. (2015)
  • Author(s): Hitoshi Ueno, Mahito Sato, Maiko Takahashi, Yuki Tajika, Tohru Murakami & Hiroshi Yorifuji
  • Organizer: The 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The 3D-atlas of adult zebrafish. (2015)
  • Author(s): Yuki Tajika, Tohru Murakami, Maiko Takahashi, Hitoshi Ueno & Hiroshi Yorifuji
  • Organizer: The 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] The expression and localization of VAMP protein in the kidney. (2015)
  • Author(s): Maiko Takahashi, Yuki Tajika, Hitoshi Ueno, Tohru Murakami & Hiroshi Yorifuji
  • Organizer: The 120th Annual Meeting of the Japanese Association of Anatomists and the 92nd Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2015-03-21 – 2015-03-23