| Project/Area Number |
26460273
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Gifu University |
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Principal Investigator |
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| Research Collaborator |
WATANABE Natsuki 岐阜大学, 大学院医学系研究科, 大学院生
WAKAOKA Takanori 岐阜大学, 大学院医学系研究科, 大学院生
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 上皮-間葉系転換 / EMT / 神経堤細胞 / Neural Crest / 直接転換法 / ダイレクトリプログラミング / 転写因子SOX10 / 間葉系細胞 / 直接転換 / レトロウィルス / 上皮-間充織転換 / Sox10 / 蛍光タンパク質 |
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| Outline of Final Research Achievements |
We revealed genes including transcription factors selectively expressed in epithelial-mesenchymal transition (EMT). Among these transcription factor genes tested, SOX10 and SOX9 were capable of converting mouse embryonic fibroblasts (MEFs) into SOX10-positive (SOX10+) cells. The SOX10+ cells were found to maintain the expression of the Neural Crest (NC) cell marker genes, P75, Foxd3 and Pax3, and were shown to differentiate into neurons, glial cells, smooth muscle cells, adipocytes and osteoblasts, suggesting that SOX10 and SOX9 directly converted MEFs into NC-like cells.
Furthermore, four transcription factors among the transcription factor genes expressed in EMT induced EMT in keratinocytes. Keratinocytes were converted into NC-like cells by overexpressing SOX10 concomitant with the EMT induction.
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