• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of Epithelial-Mesenchymal Transition (EMT) Mechanism by using EMT model system.

Research Project

Project/Area Number 26460273
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General anatomy (including histology/embryology)
Research InstitutionGifu University

Principal Investigator

MOTOHASHI Tsutomu  岐阜大学, 大学院医学系研究科, 講師 (40334932)

Research Collaborator WATANABE Natsuki  岐阜大学, 大学院医学系研究科, 大学院生
WAKAOKA Takanori  岐阜大学, 大学院医学系研究科, 大学院生
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2015: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2014: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords上皮-間葉系転換 / EMT / 神経堤細胞 / Neural Crest / 直接転換法 / ダイレクトリプログラミング / 転写因子SOX10 / 間葉系細胞 / 直接転換 / レトロウィルス / 上皮-間充織転換 / Sox10 / 蛍光タンパク質
Outline of Final Research Achievements

We revealed genes including transcription factors selectively expressed in epithelial-mesenchymal transition (EMT). Among these transcription factor genes tested, SOX10 and SOX9 were capable of converting mouse embryonic fibroblasts (MEFs) into SOX10-positive (SOX10+) cells. The SOX10+ cells were found to maintain the expression of the Neural Crest (NC) cell marker genes, P75, Foxd3 and Pax3, and were shown to differentiate into neurons, glial cells, smooth muscle cells, adipocytes and osteoblasts, suggesting that SOX10 and SOX9 directly converted MEFs into NC-like cells.
Furthermore, four transcription factors among the transcription factor genes expressed in EMT induced EMT in keratinocytes. Keratinocytes were converted into NC-like cells by overexpressing SOX10 concomitant with the EMT induction.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (14 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (1 results) Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 4 results,  Acknowledgement Compliant: 3 results) Presentation (6 results) Book (1 results) Remarks (1 results)

  • [Int'l Joint Research] National Institute on Aging, NIH(米国)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Galectin-1 enhances the generation of neural crest cells2017

    • Author(s)
      Motohashi Tsutomu、Nishioka Masahiro、Kitagawa Daisuke、Kawamura Norito、Watanabe Natsuki、Wakaoka Takanori、Kadoya Toshihiko、Kunisada Takahiro
    • Journal Title

      The International Journal of Developmental Biology

      Volume: 61 Issue: 6-7 Pages: 407-413

    • DOI

      10.1387/ijdb.160380tm

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multipotency of melanoblasts isolated from murine skin depends on the Notch signal pathway.2016

    • Author(s)
      Natsuki Watanabe, Tsutomu Motohashi, Masahiro Nishioka, Norito Kawamura, Tomohisa Hirobe, and Takahiro Kunisada
    • Journal Title

      Developmental Dynamics.

      Volume: 245 Issue: 4 Pages: 460-471

    • DOI

      10.1002/dvdy.24385

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells.2016

    • Author(s)
      Tsutomu Motohashi, Natsuki Watanabe, Masahiro Nishioka, Yuhki Nakatake, Piao Yulan, Hiromi Mochizuki, Yoshifumi Kawamura, Minoru S. H. Ko, Naoki Goshima and Takahiro Kunisada
    • Journal Title

      Biology Open

      Volume: 5 Issue: 3 Pages: 311-322

    • DOI

      10.1242/bio.015735

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Extended Multipotency of Neural Crest Cells and Neural Crest-Derived Cells.2015

    • Author(s)
      Tsutomu Motohashi, Takahiro Kunisada
    • Journal Title

      Current Topics in Developmental Biology

      Volume: 111 Pages: 69-95

    • DOI

      10.1016/bs.ctdb.2014.11.003

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] The stemness of neural crest cells and their derivatives.2014

    • Author(s)
      Kunisada T, Tezulka K, Aoki H, Motohashi T
    • Journal Title

      Birth Defects Res C Embryo Today.

      Volume: 102 Issue: 3 Pages: 251-261

    • DOI

      10.1002/bdrc.21079

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Direct conversion of mouse somatic cells into neural crest cells2017

    • Author(s)
      Tsutomu Motohashi
    • Organizer
      第40回日本分子生物学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 網羅的遺伝子発現解析による神経堤細胞への直接転換に関係する転写因子の同定2016

    • Author(s)
      本橋力
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] Gene array analysis of neural crest cells identifies transcription factors necessary for direct conversion of embryonic fibroblasts into neural crest cells.2016

    • Author(s)
      本橋力
    • Organizer
      第27回日本色素細胞学会学術大会
    • Place of Presentation
      岐阜市
    • Year and Date
      2016-11-12
    • Related Report
      2016 Research-status Report
  • [Presentation] Generation of neural crest cells by direct lineage conversion.2015

    • Author(s)
      Tsutomu Motohashi
    • Organizer
      48th Annual Meeting for the Japanese Society of Developmental Biologists
    • Place of Presentation
      つくば市
    • Year and Date
      2015-06-03
    • Related Report
      2015 Research-status Report
  • [Presentation] Neural Crest cells sustain their multipotency throughout embryogenesis.2014

    • Author(s)
      Tsutomu Motohashi, Natsuki Watanabe, Takahiro Kunisada
    • Organizer
      Grobal Controls in STEM CELLS
    • Place of Presentation
      Singapore, Biopolis
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Research-status Report
  • [Presentation] Notch signal plays important roles for the multipotency of melanoblasts.2014

    • Author(s)
      Natsuki Watanabe, Tsutomu Motohashi, Tomohisa Hirobe, Takahiro Kunisada
    • Organizer
      Grobal Controls in STEM CELLS
    • Place of Presentation
      Singapore, Biopolis
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Research-status Report
  • [Book] 色素細胞 第2版2015

    • Author(s)
      國貞隆弘、吉田尚弘、青木仁美、本橋力(他40名)
    • Total Pages
      310
    • Publisher
      慶應義塾大学出版会
    • Related Report
      2015 Research-status Report
  • [Remarks] 岐阜大学大学院、医学系研究科、組織・器官形成分野

    • URL

      http://www1.gifu-u.ac.jp/~saisei2/

    • Related Report
      2017 Annual Research Report 2016 Research-status Report 2015 Research-status Report 2014 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi