| Project/Area Number |
26460278
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TOYOSHIMA Fumiyo 宮崎大学, 医学部, 講師 (10468035)
TAKAHASHI Nobuyasu 宮崎大学, 医学部, 助教 (20404436)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 高圧凍結技法 / 上皮更新 / 超微細形態 / 電子顕微鏡 / 胃粘膜傷害 |
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| Outline of Final Research Achievements |
We investigated ultrastructural change in epithelial cell exfoliation and reconstruction by taking advantages of original cell exfoliation experimental model. As results, exfoliating cell exhibited drastic morphological changes in the subcellular organelle, plasma membrane, cytoskeleton, and autophagosomes processed by high-pressure freezing. Moreover, electron microscopy revealed ultrastructural changes of adhesion complex, and cytoskeletal dynamics in the cell process extending from the neighboring cells. In this study, we also verified that both H2 receptor blocker and proton pump inhibitor reduced the number of cell exfoliation. Taken together, the present results are highly anticipated to reach a solution for gastric mucosal injury due to H. Pylori infection, etc.
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