Involvements of Cav1.3 in the generation of a sustained inward Na+ current in sinoatrial node cells
| Project/Area Number |
26460295
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
General physiology
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松浦 博 滋賀医科大学, 医学部, 教授 (60238962)
林 維光 滋賀医科大学, 医学部, 助教 (80242973)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 心臓 / ペースメーカー活動 / イオンチャネル / ペースメーカー / 洞房結節細胞 / 電気生理学 / 心筋細胞 |
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| Outline of Final Research Achievements |
The sustained inward Na+ current has been recorded in sinoatrial node cells and put foreword as a potential pacemaker current in the heart. However, molecular determinants for Ist remain unknown. The present study has investigated the involvement of Cav1.3 L-type Ca2+ channels in the generation of Ist. We found that genetic ablation of Cav1.3 resulted in total abolition of Ist. Then we applied a next-generation sequencing approach to examine potential splicing and RNA editing of Cav1.3 in sinoatrial node, but failed to provide evidence for the presence of variants that can produce a Na current. These results raise the possibility that Cav1.3 is functionally coupled to unknown Na-permable pathway to mediate Ist in cardiac pacemaker cells.
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Report
(5 results)
Research Products
(18 results)
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[Journal Article] Novel mutations in the gene for α-subunit of retinal cone cyclic nucleotide-gated channels in a Japanese patient with congenital achromatopsia.2016
Author(s)
Kuniyoshi K, Muraki-Oda S, Ueyama H, Toyoda F, Sakuramoto H, Ogita H, Irifune M, Yamamoto S, Nakao A, Tsunoda K, Iwata T, Ohji M, Shimomura Y.
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Journal Title
Jpn J Ophthalmol
Volume: 60
Issue: 3
Pages: 187-197
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Long QT Syndrome Type8: Novel CACNA1C Mutations Causing QT Prolongation and Variant Phenotypes.2014
Author(s)
Fukuyama M, Wang Q, Kato K, Ohno S, Ding WG, Toyoda F, Itoh H, Kimura H, Makiyama T, Ito M, Matsuura H, Horie M.
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Journal Title
Europace.
Volume: in press
Issue: 12
Pages: 1828-37
DOI
Related Report
Peer Reviewed / Open Access
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