| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
It has been known that adult brain has neural stem cells residing in the niche such as subventricular zone. Cerebral blood vessels are considered to be a source of soluble factors to interact with neural stem cells (NSCs). To clarify this, we conducted coculture experiment using cerebral blood vessel derived endothelial cell line and NSCs. We found that endothelial cell derived extracellular vesicles (E-EVs) included many kind of micro RNAs (miRNAs). In silico and in vitro studies showed that some of them could suppress the expression level of transcription factors that play an important role in neuronal differentiation. By real-time PCR analyses, the endothelial cell line produced a ligand that can activate a receptor type tyrosine kinase (RTK) expressed on the NSCs. Accordingly, we conclude that cerebral endothelial cells supply the two types of soluble factors as a niche for NSCs, which are miRNAs included in E-EVs and the ligand for activating the RTK.
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