Elucidation of the vascular niche for the neural stem cells
Project/Area Number |
26460360
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General medical chemistry
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Research Institution | University of Toyama |
Principal Investigator |
Yamamoto Seiji 富山大学, 大学院医学薬学研究部(医学), 助教 (10456361)
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Co-Investigator(Kenkyū-buntansha) |
笹原 正清 富山大学, 大学院医学薬学研究部(医学), 教授 (20154015)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 血管 / 神経幹細胞 / EV / niche / RTKシグナル / miRNA / 神経 |
Outline of Final Research Achievements |
It has been known that adult brain has neural stem cells residing in the niche such as subventricular zone. Cerebral blood vessels are considered to be a source of soluble factors to interact with neural stem cells (NSCs). To clarify this, we conducted coculture experiment using cerebral blood vessel derived endothelial cell line and NSCs. We found that endothelial cell derived extracellular vesicles (E-EVs) included many kind of micro RNAs (miRNAs). In silico and in vitro studies showed that some of them could suppress the expression level of transcription factors that play an important role in neuronal differentiation. By real-time PCR analyses, the endothelial cell line produced a ligand that can activate a receptor type tyrosine kinase (RTK) expressed on the NSCs. Accordingly, we conclude that cerebral endothelial cells supply the two types of soluble factors as a niche for NSCs, which are miRNAs included in E-EVs and the ligand for activating the RTK.
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Report
(4 results)
Research Products
(28 results)
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[Journal Article] Isoliquiritigenin attenuates adipose tissue inflammation in vitro and adipose tissue fibrosis through inhibition of innate immune responses in mice.2016
Author(s)
Watanabe Y, Nagai Y, Honda H, Okamoto N, Yamamoto S, Hamashima T, Ishii Y, Tanaka M, Suganami T, Sasahara M, Miyake, K, Takatsu K.
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Journal Title
Scientific Reports
Volume: 6
Issue: 1
Pages: 23097-23097
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] PDGFR-β Plays a Key Role in the Ectopic Migration of Neuroblasts in Cerebral Stroke.2016
Author(s)
Sato H, Ishii Y, Yamamoto S, Azuma E, Takahashi Y, Hamashima T, Umezawa A, Mori H, Kuroda S, Endo S, Sasahara M.
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Journal Title
Stem Cells.
Volume: 34
Issue: 3
Pages: 685-698
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] MicroRNA-93 may control vascular endothelial growth factor A in circulating peripheral blood mononuclear cells in acute Kawasaki Disease2016
Author(s)
Saito K, Nakaoka H, Takasaki I, Hirono K, Yamamoto S, Kinoshita K, Miyao N, Ibuki K, Ozawa S, Watanabe K, Bowles NE, Ichida F
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Journal Title
Pediatr Res
Volume: 80
Issue: 3
Pages: 425-432
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] HIF-1α in Myeloid Cells Promotes Adipose Tissue Remodeling Toward Insulin Resistance.2016
Author(s)
Takikawa A, Mahmood A, Nawaz A, Kado T, Okabe K, Yamamoto S, Arif A, Senda S, Tsuneyama K, Ikutani M, Watanabe Y, Igarashi Y, Nagai Y, Takatsu K, Koizumi K, Imura J, Goda N, Sasahara M, Matsumoto M, Saeki K, Nakagawa T, Fujisaka S, Usui I, Tobe K.
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Journal Title
Diabetes
Volume: 65(12)
Issue: 12
Pages: 3649-3659
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Inflammation-induced endothelial cell-derived extracellukar vesicles modulate the cellular status of pericytes.2015
Author(s)
Yamamoto S., Niida S., Azuma E., Yanagibashi T., Muramatsu M., Huang TT., Sagara H., Higaki S., Ikutani M., Nagai Y., Takatsu K., Miyazaki K., Hamashima T., Mori H., Matsuda N., Ishii Y., Sasahara M.
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Journal Title
Scientific Reports
Volume: 5
Issue: 1
Pages: 8505-8505
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Discovery of power-law growth in the self-renewal of heterogeneous glioma stem cell populations.2015
Author(s)
Sugimori, M., Hayakawa, H., Boman, B.M., Fields, J.Z., Awaji, M., Kozano, H., Tamura, R., Yamamoto, S., Ogata, T., Yamada, M., Endo, S., Kurimoto, M. and Kuroda, S.
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Journal Title
PLoS ONE
Volume: 10
Issue: 8
Pages: e0135760-e0135760
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] PDGFRα plays a crucial role in connective tissue remodeling.2015
Author(s)
Horikawa S, Ishii Y, Hamashima T, Yamamoto S, Mori H, Fujimori T, Shen J, Inoue R, Nishizono H, Itoh H, Majima M, Abraham D, Miyawaki T, Sasahara M.
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Journal Title
Scientific Reports
Volume: Dec 7;5
Issue: 1
Pages: 17948-17948
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Presentation] Developed novel rodent model of diabetic retinopathy after conditional Pdgfrb gene targeting2016
Author(s)
Kitahara H, Yamamoto S, Kajikawa S, Azuma E, Hamashima T, Ishii Y, Sato H, Shimada Y, Sasahara M
Organizer
The 19th International Vascular Biology Meeting
Place of Presentation
Boston, Massachusetts, USA
Year and Date
2016-10-30
Related Report
Int'l Joint Research
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[Presentation] Involvement of PDGFR-beta in blood-brain barrier function after cerebral ischemia2014
Author(s)
Shen J, Ishii Y, OuYang X, Yuan J, Zhu R, Xu G, Dang TC, Hamashima T, Matsushima T, Yamamoto S, Sasahara M
Organizer
The 18th International Vascular Biology Meeting
Place of Presentation
Kyoto
Year and Date
2014-04-14 – 2014-04-17
Related Report
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