"Brain-type" glycosylation: A tag for diagnostic markers of central nervous system diseases
| Project/Area Number |
26460394
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Fukushima Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
苅谷 慶喜 福島県立医科大学, 医学部, 准教授 (00458217)
伊藤 浩美 福島県立医科大学, 医学部, 講師 (00450669)
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| Project Period (FY) |
2014-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 脳炎 / 脳症 / けいれん性疾患 / α2マクログロブリン / 髄液 / 全身性エリテマトーデス / 中枢神経障害 / 急性散在性脳脊髄炎 / α２マクログロブリン / 急性散財性脳脊髄炎 / 脳炎／脳症 / 熱性けいれん / 診断マーカー |
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| Outline of Final Research Achievements |
Alpha2-macroglobulin (α2M) is a glycoprotein in the blood and increases at early stages of inflammation. α2MG is a potent endogenous inhibitor for proteases and prevents harmful expansion of inflammation. In the present study, we quantified levels of α2MG in cerebrospinal fluid (CSF) of central nervous system diseases; i.e., acute disseminated encephalomyelitis (ADEM) and neuropsychiatric systemic lupus erythematous (NPSLE). The levels of α2MG in CSF of NPSLE were significantly higher than those of non-NPSLE, indicating that α2MG is a diagnostic marker for NPSLE. At early stage of ADEM, the levels of α2MG in CSF were significantly higher than those of benign febrile seizure (FS), suggesting that α2MG is a marker at early stage of ADEM. Thus, we concluded that α2MG could be a marker for inflammation in central nervous system.
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| Academic Significance and Societal Importance of the Research Achievements |
小児の発熱は頻度の高い症状である。この中には単なる熱発や熱性けいれんとともに、命にかかわる脳炎や脳症などが含まれることがある。例えば、脳症であれば発病の1日目に適切な治療を行えば80%が救命されるが、2日目の診断では40%、3日目の診断では10%にまで救命率が低下する。従って、脳症の病初期における診断は極めて重要であるが、従来は良い診断マーカーが知られていなかった。本研究で、脳内で生合成されるα2Mが脳炎・脳症のマーカーになることが示された。今後、イムノクロマト法でのベッドサイド診断による迅速診断が期待される。
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Report
(5 results)
Research Products
(46 results)
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[Journal Article] Spontaneous intracranial hypotension is diagnosed by a combination of lipocalin-type prostaglandin D synthase and brain-type transferrin in cerebrospinal fluid2018
Author(s)
Yuta Murakami, Koichi Takahashi, Kyoka Hoshi, Hiromi Ito, Mayumi Kanno, Kiyoshi Saito, Kenneth Nollet, Yoshiki Yamaguchi, Masakazu Miyajima, Hajime Arai, Yasuhiro Hashimoto, and Tatsuo Mima
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Journal Title
BBA General Subjects
Volume: -
Related Report
Peer Reviewed
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[Journal Article] Rapid increase of “brain-type” transferrin in cerebrospinal fluid after shunt surgery for idiopathic normal pressure hydrocephalus: a prognosis marker for cognitive recovery2018
Author(s)
Yuta Murakami, Yuka Matsumoto, Kyoka Hoshi, Hiromi Ito, Takashi J. Fuwa, Yoshiki Yamaguchi, Madoka Nakajima, Masakazu Miyajima, Hajime Arai, Kenneth Nollet, Naho Kato, Rie Nishikata, Naohito Kuroda, Takashi Honda, Jun Sakuma, Kiyoshi Saito and Yasuhiro Hashimoto
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Journal Title
Journal of Biochemistry
Volume: -
NAID
Related Report
Peer Reviewed
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[Journal Article] Excess APP O-glycosylation by GalNAc-T6 decreases Aβ production.2017
Author(s)
Keiko Akasaka-Manya, Masaki Kawamura, Hiroki Tsumoto, Yuko Saitoh, Yuriko Tachida, Shinobu Kitazume, Hiroyuki Hatsuta, Yuri Miura, Shin-ichi Hisanaga, Shigeo Murayama, Yasuhiro Hashimoto, Hiroshi Manya, Tamao Endo
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Journal Title
J. Biochem.
Volume: 161
Issue: 1
Pages: 99-111
DOI
NAID
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Subgroup differences in “brain-type” transferrin and alpha-synuclein in Parkinson’s disease and multiple system atrophy2016
Author(s)
Akioh Yoshihara, Masahiko Fukatsu, Kyoka Hoshi, Hiromi Ito, Kenneth Nollet, Yoshiki Yamaguchi, Ryotaro Ishii, Takahiko Tokuda, Masakazu Miyajima, Hajime Arai, Takeo Kato, Katsutoshi Furukawa, Hiroyuki Arai, Akio Kikuchi, Atsushi Takeda, Yoshikazu Ugawa and Yasuhiro Hashimoto
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Journal Title
J. Biochem.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] In situ visualization of a glycoform of transferrin: Localization of α2,6-sialylated transferrin in the liver2015
Author(s)
Yuka Matsumoto, Toshie Saito, Kyoka Hoshi, Hiromi Ito, Yoshinobu Kariya, Masamichi Nagae, Yoshiki Yamaguchi, Yoshiaki Hagiwara, Noriaki Kinoshita, Ikuo Wada, Kiyoshi Saito, Takashi Honda and Yasuhiro Hashimoto
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Journal Title
J. Biochem.
Volume: 157(4)
Issue: 4
Pages: 211-216
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] Subgroup differences in “brain-type” transferrin and alpha-synuclein in Parkinson’s disease and multiple system atrophy2015
Author(s)
Akioh Yoshihara, Masahiko Fukatsu, Kyoka Hoshi, Hiromi Ito, Ryotaro Ishii, Takahiko Tokuda, Masakazu Miyajima, Hajime Arai, Takeo Kato, Katsutoshi Furukawa, Hiroyuki Arai, Akio Kikuchi, Atsushi Takeda, Yoshiki Yamaguchi, Yoshikazu Ugawa and Yasuhiro Hashimoto
Organizer
7th Asian Community of Glycoscience and Glycotechnology Conference (ACGG)
Place of Presentation
Matsushima, Miyagi
Year and Date
2015-11-12
Related Report
Int'l Joint Research
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[Presentation] 髄液中トランスフェリン測定の意義2015
Author(s)
星 京香，吉原章王, 深津真彦，伊藤浩美，石井亮太郎，徳田隆彦，宮嶋雅一，新井 一，加藤丈夫，古川勝敏，荒井啓行，菊池昭夫，武田 篤，山口芳樹，宇川義一，橋本康弘
Organizer
第9回東北糖鎖研究会発表会・第9回東北薬科大学分子生体膜研究所(IMBG)シンポジウム
Place of Presentation
仙台
Year and Date
2015-09-04
Related Report
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