| Project/Area Number |
26460421
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Human pathology
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
村田 雅樹 札幌医科大学, 医学部, 講師 (10404592)
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| Co-Investigator(Renkei-kenkyūsha) |
SAWADA NORIMASA 札幌医科大学, 医学部, 教授 (30154149)
TANAKA SATOSHI 札幌医科大学, 医学部, 講師 (30374250)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 子宮頚部 / 子宮頚部腺癌 / タイト結合 / 治療・診断マーカー / 子宮頸部腺癌 / 子宮頸部 |
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| Outline of Final Research Achievements |
We exam the expression of tight junction proteins in uterine cervical adenocarcinoma specimens. We found that expression of Claudin-1 and JAM-A was significantly higher in cervical adenocarcinoma than in non-neoplastic glands. In adenocarcinoma, localization of Claudin-1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. Immunoreactivities and localization of Claudin-1 and JAM-A successfully distinguished neoplasms from non-neoplastic cervical glands with high specificity and high sensitivity. In cervical adenocarcinoma cell lines, gene knockout of Claudin-1 suppressed tumorigenicity, invasive capacity and tumor-initiation of cancer cells. Expression of Claudin-1 was mediated by ERK signaling. Targeting of Claudin-1 may be effective for cervical adenocarcinoma diagnosis and therapy.
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