Evaluation of potential clinical application of tight junction proteins in cervical adenocarcinoma
Project/Area Number |
26460421
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Human pathology
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Research Institution | Sapporo Medical University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
村田 雅樹 札幌医科大学, 医学部, 講師 (10404592)
|
Co-Investigator(Renkei-kenkyūsha) |
SAWADA NORIMASA 札幌医科大学, 医学部, 教授 (30154149)
TANAKA SATOSHI 札幌医科大学, 医学部, 講師 (30374250)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | 子宮頚部 / 子宮頚部腺癌 / タイト結合 / 治療・診断マーカー / 子宮頸部腺癌 / 子宮頸部 |
Outline of Final Research Achievements |
We exam the expression of tight junction proteins in uterine cervical adenocarcinoma specimens. We found that expression of Claudin-1 and JAM-A was significantly higher in cervical adenocarcinoma than in non-neoplastic glands. In adenocarcinoma, localization of Claudin-1 and JAM-A was extended throughout the whole cell membranes, whereas they were predominantly expressed at the most apical cell-cell junction in non-neoplastic glands. Immunoreactivities and localization of Claudin-1 and JAM-A successfully distinguished neoplasms from non-neoplastic cervical glands with high specificity and high sensitivity. In cervical adenocarcinoma cell lines, gene knockout of Claudin-1 suppressed tumorigenicity, invasive capacity and tumor-initiation of cancer cells. Expression of Claudin-1 was mediated by ERK signaling. Targeting of Claudin-1 may be effective for cervical adenocarcinoma diagnosis and therapy.
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Report
(4 results)
Research Products
(10 results)
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[Journal Article] Microenvironmental stresses induce HLA-E/Qa-1 surface expression and thereby reduce CD8+ T-cell recognition of stressed cells2016
Author(s)
Sasaki T, Kanaseki T, Shionoya Y, Tokita S, Miyamoto S, Saka E, Kochin V, Takasawa A, Hirohashi Y, Tamura Y, Miyazaki A, Torigoe T, Hiratsuka H, Sato N
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Journal Title
Eur J Immunol
Volume: 46
Issue: 4
Pages: 929-940
DOI
Related Report
Peer Reviewed
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[Journal Article] Nuclear localization of tricellulin promotes the oncogenic property of pancreatic cancer2016
Author(s)
Akira Takasawa, Masaki Murata, Kumi Takasawa, Yusuke Ono, Makoto Osanai, Satoshi Tanaka, Masanori Nojima, Tsuyoshi Kono, Koichi Hirata, Takashi Kojima, Norimasa Sawada
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Journal Title
Scientific Reports
Volume: -
Issue: 1
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Analysis of the expression and localization of tight junction transmembrane proteins, claudin-1, -4, -7, occludin and JAM-A, in human cervical adenocarcinoma.2016
Author(s)
Akimoto T, Takasawa A, Murata M, Kojima Y, Takasawa K, Nojima M, Aoyama T, Hiratsuka Y, Ono Y, Tanaka S, Osanai M, Hasegawa T, Saito T, Sawada N.
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Journal Title
Histology and Histopathology
Volume: in press
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Aberrant expression of claudin-4 and -7 in hepatocytes in the cirrhotic human liver.2015
Author(s)
Tsujiwaki M, Murata M, Takasawa A, Hiratsuka Y, Fukuda R, Sugimoto K, Ono Y, Nojima M, Tanaka S, Hirata K, Kojima T, Sawada N.
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Journal Title
Med Mol Morphol.
Volume: 48(1)
Issue: 1
Pages: 33-43
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] An immunohistochemical marker panel including claudin-18, maspin, and p53 improves diagnostic accuracy of bile duct neoplasms in surgical and presurgical biopsy specimens.2015
Author(s)
Keira Y, Takasawa A, Murata M, Nojima M, Takasawa K, Ogino J, Higashiura Y, Sasaki A, Kimura Y, Mizuguchi T, Tanaka S, Hirata K, Sawada N, Hasegawa T.
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Journal Title
Virchows Arch.
Volume: 466(3)
Issue: 3
Pages: 265-277
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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