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Research on prediction of persistent infection of HPV and improvement of sensitivity of cytodiagnosis using cervical scrape cells.

Research Project

Project/Area Number 26460459
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Human pathology
Research InstitutionKyorin University

Principal Investigator

Okodo Mitsuaki  杏林大学, 保健学部, 准教授 (10276206)

Co-Investigator(Kenkyū-buntansha) 藤井 雅彦  杏林大学, 保健学部, 教授 (50108065)
岡山 香里  群馬パース大学, 保健科学部, 助教 (10780116)
Research Collaborator ODA Mizue  こころとからだの元気プラザ
FUJII Masahiko  こころとからだの元気プラザ
Project Period (FY) 2014-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2016: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords子宮頸部上皮内腫瘍 / HPV / 持続感染 / 単独感染 / ハイリスクHPV / 子宮頸部細胞診 / integration / ハイリスク型HPV
Outline of Final Research Achievements

In this study, we developed a new assay (uniplex E6 / E7 PCR assay) to accurately detect single infection of human papilloma virus (HPV) type 16. As a result of analysis of cervical scrape cells in this assay,(1) Most cases were multiple infections in cases where it was determined to be single infection by type 16 by a commercially available examination method,(2) In CIN 1, there were very few HPV type 16 single infection cases,(3) Patients with HPV type 16 single infection were lesions with CIN 2 or higher,(4) In HPV type 16 single infection cases, HPV was integrated in the human genome.
From the results of this study, we concluded that it is important to detect single infection with appropriate assay to predict continuous infection of HPV and progression of lesion.

Report

(5 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 2015 2014

All Journal Article (4 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (13 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types2018

    • Author(s)
      Okodo Mitsuaki、Okayama Kaori、Teruya Koji、Sasagawa Toshiyuki
    • Journal Title

      Journal of Medical Virology

      Volume: 90 Issue: 5 Pages: 981-988

    • DOI

      10.1002/jmv.25017

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Significance of compression in binucleation while differentiating reactive cellular changes between human papillomavirus and Candida Infections.2017

    • Author(s)
      Okodo M, Okayama K, Fukui T, Shiina N, Caniz T, Yabusaki H, Fujii M.
    • Journal Title

      Asian Pac J Cancer Prev

      Volume: 18 Pages: 2507-2511

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluating the existence of small compressed binucleated squamous cells in ASC-H.2016

    • Author(s)
      Okodo M, Okayama K, Kitamura H, Shiina N, Caniz T, Ono M, Yabusaki H
    • Journal Title

      Asian Pacific Journal of Cancer Prevention

      Volume: 17 Pages: 4665-4669

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] HPVに特徴的な細胞像2016

    • Author(s)
      岡山香里、大河戸光章
    • Journal Title

      産婦人科の実際

      Volume: 65 Pages: 1511-1517

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] HPVE6・E7遺伝子領域をターゲットとした型特異的プライマーによるuniplex PCR アッセイ2017

    • Author(s)
      大河戸光章, 岡山香里, 笹川寿之
    • Organizer
      第56回日本臨床細胞学会秋期大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 細胞検査士要望教育シンポジウム: 婦人科細胞診におけるin situ PCR法2017

    • Author(s)
      岡山香里, 大河戸光章
    • Organizer
      第56回日本臨床細胞学会秋期大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 日本における高リスクHPV型の同定と検診への応用の可能性2017

    • Author(s)
      坂本人一, 大阪康宏, 柴田健雄, 岡山香里, 大河戸光章, 笹川 寿之
    • Organizer
      第58回日本臨床細胞学会春期大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 子宮頸部および肛門管細胞診におけるHPV感染2016

    • Author(s)
      大河戸光章
    • Organizer
      日本臨床細胞学会埼玉県支部会
    • Place of Presentation
      埼玉
    • Year and Date
      2016-03-05
    • Related Report
      2015 Research-status Report
    • Invited
  • [Presentation] 子宮頸部におけるユニークハイリスク型HPVの感染経過2016

    • Author(s)
      大河戸光章・岡山香里
    • Organizer
      第7回日本性感染症学会北陸支部学術講演会
    • Place of Presentation
      金沢
    • Related Report
      2016 Research-status Report
  • [Presentation] 日本人男性間性交渉者における肛門HPV感染および肛門上皮細胞異型に関する検討(会議録)2015

    • Author(s)
      八重樫 洋, 重原 一慶, 中嶋 一史, 川口 昌平, 井戸田 一朗, 大河戸 光章, 笹川 寿之, 並木 幹夫
    • Organizer
      日本性感染症学会
    • Place of Presentation
      東京
    • Year and Date
      2015-12-05
    • Related Report
      2015 Research-status Report
  • [Presentation] 拡大肛門鏡を用いたHIV陽性者の肛門管上皮内病変の診断と治療(会議録)2015

    • Author(s)
      北村 浩, 井戸田 一朗, 岡山 香里, 大河戸 光章, 佐野 彰彦, 佐野 麻里子, 小林 治
    • Organizer
      日本エイズ学会
    • Place of Presentation
      東京
    • Year and Date
      2015-11-30
    • Related Report
      2015 Research-status Report
  • [Presentation] HIV陽性MSMにおける肛門擦過細胞診とHuman papillomavirus検査(会議録)2015

    • Author(s)
      大河戸 光章, 岡山 香里, 北村 浩, 井戸田 一朗
    • Organizer
      日本エイズ学会
    • Place of Presentation
      東京
    • Year and Date
      2015-11-30
    • Related Report
      2015 Research-status Report
  • [Presentation] HIV陽性men who have sex with men(MSM)における肛門擦過細胞診(会議録)2015

    • Author(s)
      岡山 香里, 大河戸 光章, 北村 浩, 井戸田 一朗
    • Organizer
      日本臨床細胞学会
    • Place of Presentation
      名古屋
    • Year and Date
      2015-11-21
    • Related Report
      2015 Research-status Report
  • [Presentation] 子宮頸部上皮内腫瘍においてハイリスクが疑われるマイナーHPVの検出状2015

    • Author(s)
      大河戸 光章(杏林大学 保健学部病理学研究室), 岡山 香里, 笹川 寿之, 小田 瑞恵, 栗原 達哲, 石井 保吉, 藤井 雅彦
    • Organizer
      日本臨床細胞学会
    • Place of Presentation
      松江
    • Year and Date
      2015-06-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 肛門管擦過細胞診標本における多彩なHPV感染所見(会議録)2015

    • Author(s)
      岡山 香里, 大河戸 光章, 北村 浩, 井戸田 一朗, 藤井 雅彦
    • Organizer
      日本臨床細胞学会
    • Place of Presentation
      松江
    • Year and Date
      2015-06-12
    • Related Report
      2015 Research-status Report
  • [Presentation] 子宮頸部上皮内腫瘍における新しいハイリスク型HPVの検出状況2014

    • Author(s)
      大河戸光章
    • Organizer
      京都・金沢 STIエキスパートミーティング
    • Place of Presentation
      京都
    • Year and Date
      2014-06-13
    • Related Report
      2014 Research-status Report
    • Invited
  • [Presentation] 細胞診標本のためのin situ PCR法2014

    • Author(s)
      岡山香里、大河戸光章、熊谷朋子、薮崎宏美、椎名義雄、吉永陽樹、福井正、藤井雅彦
    • Organizer
      第55回日本臨床細胞学会春季大会
    • Place of Presentation
      神奈川
    • Year and Date
      2014-06-06 – 2014-06-07
    • Related Report
      2014 Research-status Report
  • [Book] 臨床検査学講座 病理学/病理検査学2016

    • Author(s)
      大河戸光章
    • Total Pages
      138
    • Publisher
      医歯薬出版
    • Related Report
      2016 Research-status Report

URL: 

Published: 2014-04-04   Modified: 2019-03-29  

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