The pathophysiology in hypoadiponectinemia

• Project/Area Number: 26460494
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Yokohama College of Pharmacy (2016-2017); Showa University (2014-2015)
• Principal Investigator: NAKANO Yasuko 横浜薬科大学, 薬学部, 教授 (20155790)
• Co-Investigator(Kenkyū-buntansha): 根来 孝治 帝京平成大学, 薬学部, 講師 (70218270) ; 谷岡 利裕 昭和大学, 薬学部, 講師 (80360585)
• Co-Investigator(Renkei-kenkyūsha): TANIOKA Toshihiro 昭和大学, 薬学部, 助教 (80360585)
• Project Period (FY): 2014-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2015: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 炎症 / アディポネクチン / 生活習慣病 / 未病状態 / マクロファージ / アディポネクチントランスジェニックマウス / 低アディポネクチン血症 / 易炎症性 / 高分子量アディポネクチン / 低アディポネクチン血漿

Outline of Final Research Achievements

Adiponectin secreted from adipocyte has anti-inflammatory function and inhibits diabetes, atherogenic disease and so on. Adiponectin anti-sense transgenic mouse which we produced shows no difference with wild type mouse in plasma adipocectin concentration and under normal conditions, there is no abnormality. On the other hand, once inflammation was induced, inflammation became severer and prolonged. These suggest anti-sense transgenic mouse is in predisease condition and we have carried out comprehensive analysis. As a result, in anti-sense transgenic mouse, genes of metabolic process and energy storage were down regulated and these changes and resulted low energy production are suggested to be the cause of easy induction of inflammation and resulted severer inflammation.

Research Products

• [Journal Article] Higher Adiponectin Expression Suppresses Neointimal Hyperplasia by Attenuating the Inflammatory Response Following Acceleration of Endothelialization in Damaged Areas in Adiponectin Transgenic Mice (2017)
  • Author(s): Omori Y, Suzuki H, Saito K, Shoji M, Iso Y, Negoro T, Koba S, Nakano Y, Kobayashi Y
  • Journal Title: The Showa University Journal of Medical Sciences Volume: 29 Issue: 2 Pages: 107-117
  • DOI: 10.15369/sujms.29.107
  • NAID: 130006100252
  • ISSN: 0915-6380, 2185-0968
  • Peer Reviewed / Open Access
• [Journal Article] TRPM2 channels in alveolar epithelial cells mediate bleomycin-induced lung inflammation (2016)
  • Author(s): Yonezawa R, Yamamoto S, Takenaka M, Kage Y, Negoro T, Toda T, Ohbayashi M, Numata T, Nakano Y, Yamamoto T, Mori Y, Ishii M, Shimizu S
  • Journal Title: Free Radic Biol Med Volume: 90 Pages: 101-113
  • DOI: 10.1016/j.freeradbiomed.2015.11.021
  • Peer Reviewed
• [Journal Article] バイオマーカーとしてのアディポネクチン (2015)
  • Author(s): 中野泰子、平野勉、芳野原、菅沼理恵、土岐彰、都島基夫
  • Journal Title: 昭和学士会雑誌 Volume: 75 Pages: 256-265
  • NAID: 130005111673
  • Peer Reviewed / Open Access
• [Journal Article] Elevated receptor for activated C kinase 1 expression is involved in intracellular Ca2+ influx and potentially associated with compromised regulatory T cell function in patients with asthma (2014)
  • Author(s): Negoro T, Shimizu S, Narushima M, Banham AH, Wakabayashi H, Takayanagi R, Hagiwara T, Roncador R, Osabe T, Yanai T, Kin T, Ikeda K, Endo A, Akiyama H, Nakano Y
  • Journal Title: Clin Exp Allergy Volume: 44 Issue: 9 Pages: 1154-1169
  • DOI: 10.1111/cea.12375
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] A simple smart amplification assay for the rapid detection of human cytomegalovirus in the urine of neonates (2014)
  • Author(s): Kohda C, Chiba N, Shimokoba K, Mizuno K, Negoro T, Nakano Y, Tanaka K
  • Journal Title: J Virol Methods Volume: 208 Pages: 160-165
  • DOI: 10.1016/j.jviromet.2014.07.034
  • Peer Reviewed / Open Access
• [Presentation] アディポネクチンアンチセンス、センストランスジェニックマウスの表現型解析 (2016)
  • Author(s): 中野泰子、秋山晴代、目加田和之、若菜茂晴、齊藤清美、巖本三壽、吉木淳、根来孝治
  • Organizer: 第８９回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Presentation] 易炎症性マウスを用いた未病に関与するターゲット因子の探索 (2016)
  • Author(s): 秋山晴代、長部隆広、根来孝治、荒木真由美、瀬戸山央、青木信義、渡邊裕子、甲斐茂美、中野泰子、宮澤眞紀
  • Organizer: 日本薬学会
  • Place of Presentation: 横浜
  • Year and Date: 2016-03-26
• [Presentation] HMW Adiponectin Suppresses LPS-Induced IL-1b Expression through Inhibiting Akt-C/EBPb Inflammatory Signaling in RAW 264.7 Macrophage. (2015)
  • Author(s): Toshihiro Tanioka, Yoshinao Tainaga, Michiyo Yamada, Yuuri Kamimura, Mariko Suzuki, Saori Takatori, Masahiro Tateno, Mami Matsumot, Yasuko Nakano
  • Organizer: The 12th World Congress on Inflammation
  • Place of Presentation: Boston, Massachusetts, USA
  • Year and Date: 2015-08-08
  • Int'l Joint Research
• [Presentation] アディポネクチンによる炎症性サイトカイン抑制作用の解析 (2014)
  • Author(s): 谷岡利裕、躰長義直、山田倫世、根来孝治、中野泰子
  • Organizer: 第35回 日本炎症・再生医学会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-07-01 – 2014-07-04
• [Presentation] Adiponectin preferentially inhibit LPS-induced IL-1βexpression on macrophage. (2014)
  • Author(s): Tanioka T, Tainaga Y, Yamada Y, Negoro T, Nakano Y.
  • Organizer: The 22nd International Symposium on Molecular Cell Biology of Macrophages
  • Place of Presentation: 神戸
  • Year and Date: 2014-06-01 – 2014-06-03
• [Remarks] 横浜薬科大学臨床薬学科教員プロフィール
  • URL: http://www.hamayaku.jp/teacher/te_183.html
• [Remarks] 昭和大学薬学部薬物療法学講座遺伝解析部門
  • URL: http://www.showa-u.ac.jp/sch/pharm/major/medinfo/index.html