Analysis of non-coding RNA regulatory mechanism in B cell terminal differentiation
Project/Area Number |
26460580
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Immunology
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Research Institution | Osaka University (2015-2016) Kumamoto University (2014) |
Principal Investigator |
Maeda Kazuhiko 大阪大学, 微生物病研究所, 准教授 (20332869)
|
Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 抗体 / 体細胞突然変異 / RNA代謝 / 非コードRNA / ゲノム / 脱アミノ化酵素 / B細胞 |
Outline of Final Research Achievements |
Antibodies responsible for adaptive immunity have a variety of repertoires against diverse antigens. Although antibody-producing B cells differentiate into either memory B cells or plasma cells from germinal center B cells, however, it is not clear what kind of mechanism maintains antibody production ability for a long time period. In this process, a molecular regulation mechanism via activation-induced cytidine deaminase (AID), which is specifically expressed in mature B cells, is essential. In this research, we focused on GANP molecule, which is relevant to the protein, DNA and RNA level as a cofactor of AID, and it was possible to clarify the access genomic region and the GANP binding various mRNA/miRNA. From the results of this research, we could disclose the molecular basis of intracellular RNA controlled by GANP.
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Report
(4 results)
Research Products
(37 results)
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[Presentation] GANP that interacts with HIV-1 integrase inherently enhances retrotransposition2014
Author(s)
Shimoda, M. Maeda, K. Almofty, S. A. Singh, S. K. Eid, M. M. A. Koito, A. Sakaguchi, N.
Organizer
15th Kumamoto AIDS Seminar
Place of Presentation
Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel, Kumamoto, Japan
Year and Date
2014-10-01 – 2014-10-03
Related Report
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[Presentation] A TREX-2 component GANP is dispersed from the nuclear envelope during HIV-1 virus entry and associated with integrase in the host cell nucleus2014
Author(s)
Almofty, S. A. Maeda, K. Shimoda, M. Singh, S. K. Eid, M. M. A. Koito, A. Sakaguchi, N.
Organizer
15th Kumamoto AIDS Seminar
Place of Presentation
Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel, Kumamoto, Japan
Year and Date
2014-10-01 – 2014-10-03
Related Report
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[Presentation] Molecular mechanism of transcription and epigenetic alteration at immunoglobulin variable gene heavy chain locus induced by GANP2014
Author(s)
Singh, S. K. Maeda, K. Eid, M. M. A. Almofty, S. A. Shimoda, M Kawabata, T Sakaguchi, N.
Organizer
Immunology 2014 The American Association of Immunologists Annual Meeting
Place of Presentation
David L. Lawrence Convention Center, Pittsburgh, Pennsylvania, USA
Year and Date
2014-05-02 – 2014-05-06
Related Report
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