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Analysis of non-coding RNA regulatory mechanism in B cell terminal differentiation

Research Project

Project/Area Number 26460580
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Immunology
Research InstitutionOsaka University (2015-2016)
Kumamoto University (2014)

Principal Investigator

Maeda Kazuhiko  大阪大学, 微生物病研究所, 准教授 (20332869)

Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords抗体 / 体細胞突然変異 / RNA代謝 / 非コードRNA / ゲノム / 脱アミノ化酵素 / B細胞
Outline of Final Research Achievements

Antibodies responsible for adaptive immunity have a variety of repertoires against diverse antigens. Although antibody-producing B cells differentiate into either memory B cells or plasma cells from germinal center B cells, however, it is not clear what kind of mechanism maintains antibody production ability for a long time period. In this process, a molecular regulation mechanism via activation-induced cytidine deaminase (AID), which is specifically expressed in mature B cells, is essential. In this research, we focused on GANP molecule, which is relevant to the protein, DNA and RNA level as a cofactor of AID, and it was possible to clarify the access genomic region and the GANP binding various mRNA/miRNA. From the results of this research, we could disclose the molecular basis of intracellular RNA controlled by GANP.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (37 results)

All 2017 2016 2015 2014 Other

All Int'l Joint Research (2 results) Journal Article (8 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 8 results,  Open Access: 7 results,  Acknowledgement Compliant: 6 results) Presentation (20 results) (of which Int'l Joint Research: 3 results) Book (3 results) Remarks (4 results)

  • [Int'l Joint Research] 南カリフォルニア大学/ミネソタ大学/テキサス・リオ・グランド・バレー大学(米国)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University Sains Malaysia(Malaysia)

    • Related Report
      2015 Research-status Report
  • [Journal Article] Opossum APOBEC1 is a DNA mutator with retrovirus and retroelement restriction activity2017

    • Author(s)
      Ikeda T, Shimoda M, Ebrahimi D, VandeBerg J, Harris RS, Koito A, and Maeda K
    • Journal Title

      Sci. Reports

      Volume: 7 Issue: 1 Pages: 46719-46719

    • DOI

      10.1038/srep46719

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Regulation of mRNA stability by CCCH-type zinc finger proteins in immune cells2017

    • Author(s)
      Maeda K and Akira S
    • Journal Title

      Int. Immunol.

      Volume: - Issue: 4 Pages: 149-155

    • DOI

      10.1093/intimm/dxx015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Activation-induced deoxycytidine deaminase: structural basis favoring WRC hot motif specificities unique among APOBEC family members.2017

    • Author(s)
      Pham P, Afif SA, Shimoda M, Maeda K, Sakaguchi N, Pedersen LC, and Goodman MF
    • Journal Title

      DNA Repair (Amst)

      Volume: 54 Pages: 8-12

    • DOI

      10.1016/j.dnarep.2017.03.007

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] TLR7 structure: Cut in Z-loop2016

    • Author(s)
      Maeda K and Akira S
    • Journal Title

      Immunity

      Volume: 45 Issue: 4 Pages: 705-707

    • DOI

      10.1016/j.immuni.2016.10.003

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural analysis of the activation-induced deoxycytidine deaminase required in immunoglobulin diversification2016

    • Author(s)
      Pham P, Afif SA, Shimoda M, Maeda K, Sakaguchi N, Pedersen LC, and Goodman MF
    • Journal Title

      DNA Repair (Amst)

      Volume: 43 Pages: 48-56

    • DOI

      10.1016/j.dnarep.2016.05.029

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Creation of chimeric human/rabbit APOBEC1 with HIV-1 restriction and DNA mutation activities2016

    • Author(s)
      Ikeda T, Ong EBB, Watanabe N, Sakaguchi N, Maeda K, and Koito A.
    • Journal Title

      Sci. Reports

      Volume: 6 Issue: 1 Pages: 19035-19035

    • DOI

      10.1038/srep19035

    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Germinal center B-cell-associated nuclear protein (GANP) involved in RNA metabolism for B cell maturation2016

    • Author(s)
      Sakaguchi N and Maeda K
    • Journal Title

      Adv. Immunol

      Volume: 131 Pages: 135-186

    • DOI

      10.1016/bs.ai.2016.02.003

    • ISBN
      9780128047989
    • Related Report
      2015 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] GANP regulates the choice of DNA repair pathway by DNA-PKcs interaction in AID-dependent IgV region diversification2014

    • Author(s)
      Eid MMA, Maeda K, Almofty SA, Singh SK, Shimoda M, Sakaguchi N.
    • Journal Title

      The Journal of Immunology

      Volume: 192(印刷中) Issue: 12 Pages: 5529-5539

    • DOI

      10.4049/jimmunol.1400021

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Role of selective regulation of noncoding RNA by mammalian RNA export factor GANP2017

    • Author(s)
      Maeda K
    • Organizer
      Keystone Symposia Conference Noncoding RNAs: From Disease to Targeted Therapeutics
    • Place of Presentation
      Fairmont Banff Springs (Banff, Canada)
    • Year and Date
      2017-02-05
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Regulation of HIV-1 mRNA export by a host TREX-2 component GANP2016

    • Author(s)
      Shimoda M
    • Organizer
      The 45th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      沖縄コンベンションセンター、ラグナガーデンホテル、(沖縄県宜野湾市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Annual Research Report
  • [Presentation] Impact of RNA export component GANP on nuclear transport in viral infection2016

    • Author(s)
      Shimoda M
    • Organizer
      The 16th International Congress of Immunology
    • Place of Presentation
      Melbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Selective regulation of noncoding RNA by mediating AID-interacting molecule GANP2016

    • Author(s)
      Maeda K
    • Organizer
      The 16th International Congress of Immunology
    • Place of Presentation
      Melbourne Convention and Exhibition Centre (Melbourne, Australia)
    • Year and Date
      2016-08-20
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] GANP interacts c-myc gene and translation initiation factor 4E associated with Burkitt's lymphomagenesis2015

    • Author(s)
      Singh, S. K. Shimoda, M. Maeda, K. Sakaguchi, N.
    • Organizer
      The 44th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      札幌 コンベンションセンター
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] GANP, a component of RNA export TREX-2 complex, is involved in the retrotransposition regulation2015

    • Author(s)
      Shimoda, M Maeda, K. Almofty, S. A. Singh, S. K. Sakaguchi, N.
    • Organizer
      第9回日本エピジェネティクス研究会年会
    • Place of Presentation
      東京 学術総合センター 一橋講堂
    • Year and Date
      2015-05-25
    • Related Report
      2015 Research-status Report
  • [Presentation] Regulation of selective non-coding RNA species involved in B-cell maturation by AID-interacting molecule GANP2015

    • Author(s)
      Maeda, K. Shimoda, M Singh, S. K. Almofty, S. A. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      Keystone Symposia Conference The Golden Anniversary of B Cell Discovery
    • Place of Presentation
      Fairmont Banff Springs, Banff, Alberta, Canada
    • Year and Date
      2015-03-22 – 2015-03-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Mechanism of activation of LINE-1 retrotransposition in somatic cells2014

    • Author(s)
      Shimoda, M. Maeda, K. Almofty, S. A. Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      The 43th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kyoto International Conference Center
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] GANP takes part of the function of TREX-2 complex for exporting non-coding RNAs in B-cells2014

    • Author(s)
      Maeda, K. Almofty, S. A. Singh, S. K. Shimoda, M. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      The 43th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] APOBEC3G-associated GANP in HIV-1 virion initially interacts with the viral integration complex and might regulate viral integration into the host genome2014

    • Author(s)
      Almofty, S. A. Maeda, K. Shimoda, M. Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      The 43th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] GANP plays a critical role in the final stage of immunoglobulin V-region somatic hypermutation for generation of high-affinity antigen-specific antibody2014

    • Author(s)
      Eid, M. M. A. Maeda, K. Singh, S. K. Almofty, S. A. Shimoda, M. Sakaguchi, N.
    • Organizer
      The 43th Annual Meeting of the Japanese Society for Immunology
    • Place of Presentation
      Kyoto International Conference Center, Kyoto
    • Year and Date
      2014-12-10 – 2014-12-12
    • Related Report
      2014 Research-status Report
  • [Presentation] ヒトRNA 輸送TREX-2 因子GANP のHIV-1 抵抗性の分子機序2014

    • Author(s)
      前田和彦 アルモフティ・サーラ アミーン 下田真唯子 シン・シャイレンドラ クマール エイド・モハメド アバス 川畑輝弥 池田輝政 小糸厚 阪口薫雄
    • Organizer
      第28 回日本エイズ学会学術集会
    • Place of Presentation
      大阪国際会議場、大阪
    • Year and Date
      2014-12-03 – 2014-12-05
    • Related Report
      2014 Research-status Report
  • [Presentation] A host factor GANP involve in anti-HIV-1 infectivity response by facilitating the encapsidation of APOBEC3G into virion core2014

    • Author(s)
      Almofty, S. A. Maeda, K. Shimoda, M. Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      International Workshop on Biomedical Engineering at Osaka Institute of Technology
    • Place of Presentation
      Omiya Campus of Osaka Institute of Technology, Osaka
    • Year and Date
      2014-11-16
    • Related Report
      2014 Research-status Report
  • [Presentation] HIV-1 virus causes disassembly of TREX-2 component GANP from nuclear pores to be taken with integrase in the virus entry process2014

    • Author(s)
      Maeda, K. Almofty, S. A. Shimoda, M. Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      The 10th China-Japan Joint Laboratory Workshop: Pathogenesis, Gene Regulation and Signal Transduction
    • Place of Presentation
      Chinese Academy of Sciences, Beijing, China
    • Year and Date
      2014-11-15
    • Related Report
      2014 Research-status Report
  • [Presentation] GANP that interacts with HIV-1 integrase inherently enhances retrotransposition2014

    • Author(s)
      Shimoda, M. Maeda, K. Almofty, S. A. Singh, S. K. Eid, M. M. A. Koito, A. Sakaguchi, N.
    • Organizer
      15th Kumamoto AIDS Seminar
    • Place of Presentation
      Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel, Kumamoto, Japan
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] A TREX-2 component GANP is dispersed from the nuclear envelope during HIV-1 virus entry and associated with integrase in the host cell nucleus2014

    • Author(s)
      Almofty, S. A. Maeda, K. Shimoda, M. Singh, S. K. Eid, M. M. A. Koito, A. Sakaguchi, N.
    • Organizer
      15th Kumamoto AIDS Seminar
    • Place of Presentation
      Hotel Nikko Kumamoto, Aso Resort Grandvrio Hotel, Kumamoto, Japan
    • Year and Date
      2014-10-01 – 2014-10-03
    • Related Report
      2014 Research-status Report
  • [Presentation] Role of GANP in the DNA repair for AID-induced somatic hypermutation generating high affinity antibodies2014

    • Author(s)
      Sakaguchi, N. Eid, M. M. A. Singh, S. K. Almofty, S. A. Shimoda, M Maeda, K.
    • Organizer
      The 2nd Symposium of International Immunological Memory and Vaccine Forum
    • Place of Presentation
      La Jolla Institute for Allergy & Immunology, San Diego, CA, USA
    • Year and Date
      2014-08-25 – 2014-08-26
    • Related Report
      2014 Research-status Report
  • [Presentation] GANP-mediated APOBEC3G targeting in generation of non-infectious HIV-1viruses2014

    • Author(s)
      Maeda, K. Almofty, S. A. Shimoda, M Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      The 2nd Symposium of International Immunological Memory and Vaccine Forum
    • Place of Presentation
      La Jolla Institute for Allergy & Immunology, San Diego, CA, USA
    • Year and Date
      2014-08-25 – 2014-08-26
    • Related Report
      2014 Research-status Report
  • [Presentation] GANP plays a role in the LINE-1 life cycle in somatic cells2014

    • Author(s)
      Shimoda, M Maeda, K. Almofty, S. A. Singh, S. K. Eid, M. M. A. Sakaguchi, N.
    • Organizer
      第8 回日本エピジェネティクス研究会年会
    • Place of Presentation
      伊藤国際学術研究センター(東京大学構内)、東京
    • Year and Date
      2014-05-25 – 2014-05-27
    • Related Report
      2014 Research-status Report
  • [Presentation] Molecular mechanism of transcription and epigenetic alteration at immunoglobulin variable gene heavy chain locus induced by GANP2014

    • Author(s)
      Singh, S. K. Maeda, K. Eid, M. M. A. Almofty, S. A. Shimoda, M Kawabata, T Sakaguchi, N.
    • Organizer
      Immunology 2014 The American Association of Immunologists Annual Meeting
    • Place of Presentation
      David L. Lawrence Convention Center, Pittsburgh, Pennsylvania, USA
    • Year and Date
      2014-05-02 – 2014-05-06
    • Related Report
      2014 Research-status Report
  • [Book] 獲得免疫におけるB細胞の研究、B細胞分化・活性化、そして抗体親和性の分子機構2016

    • Author(s)
      阪口薫雄、前田和彦
    • Publisher
      羊土社
    • Related Report
      2016 Annual Research Report
  • [Book] エッセンシャル免疫学 第3版2016

    • Author(s)
      前田和彦,阪口薫雄 (分担)
    • Publisher
      メディカル・サイエンス・インターナショナル
    • Related Report
      2016 Annual Research Report
  • [Book] 免疫グロブリンの体細胞高頻度突然変異誘導とGANP2014

    • Author(s)
      下田真唯子, 前田和彦, 阪口薫雄
    • Publisher
      科学評論社
    • Related Report
      2014 Research-status Report
  • [Remarks]

    • URL

      http://www.ifrec.osaka-u.ac.jp/jpn/laboratory/shizuo_akira/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 大阪大学微生物病研究所 論文リスト

    • URL

      http://www.biken.osaka-u.ac.jp/achievement/publications/

    • Related Report
      2016 Annual Research Report
  • [Remarks] 論文

    • URL

      http://www.biken.osaka-u.ac.jp/publications_j/publications2016.html

    • Related Report
      2015 Research-status Report
  • [Remarks] 熊本大学大学院生命科学研究部 感染・免疫学講座 免疫学分野

    • URL

      http://www.k-immu.jp/ja/index.html

    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2022-02-16  

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