| Project/Area Number |
26460580
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Osaka University (2015-2016)
Kumamoto University (2014) |
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Principal Investigator |
Maeda Kazuhiko 大阪大学, 微生物病研究所, 准教授 (20332869)
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 抗体 / 体細胞突然変異 / RNA代謝 / 非コードRNA / ゲノム / 脱アミノ化酵素 / B細胞 |
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| Outline of Final Research Achievements |
Antibodies responsible for adaptive immunity have a variety of repertoires against diverse antigens. Although antibody-producing B cells differentiate into either memory B cells or plasma cells from germinal center B cells, however, it is not clear what kind of mechanism maintains antibody production ability for a long time period. In this process, a molecular regulation mechanism via activation-induced cytidine deaminase (AID), which is specifically expressed in mature B cells, is essential. In this research, we focused on GANP molecule, which is relevant to the protein, DNA and RNA level as a cofactor of AID, and it was possible to clarify the access genomic region and the GANP binding various mRNA/miRNA. From the results of this research, we could disclose the molecular basis of intracellular RNA controlled by GANP.
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