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Role of CCL28 for mucosal immunity

Research Project

Project/Area Number 26460582
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Immunology
Research InstitutionKindai University

Principal Investigator

YOSHIE Osamu  近畿大学, 医学部, 教授 (10166910)

Co-Investigator(Kenkyū-buntansha) 樋口 智紀  高知大学, 教育研究部医療学系基礎医学部門, 助教 (00448771)
金井 亨輔  近畿大学, 医学部, 助教 (20596621)
藤田 貢  近畿大学, 医学部, 准教授 (40609997)
Co-Investigator(Renkei-kenkyūsha) NAKAYAMA Takashi  近畿大学, 薬学部, 教授 (60319663)
Research Collaborator TSUNODA Ikuo  近畿大学, 医学部, 教授 (00261529)
Project Period (FY) 2014-04-01 – 2017-03-31
Project Status Completed (Fiscal Year 2016)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2015: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywordsケモカイン / CCL28 / 腸管免疫 / IgA / 腸内細菌叢 / 腸管マイクロビオーム / IgA 産生細胞 / 粘膜免疫 / 抗菌作用 / CCR10 / 腸管細菌叢 / IgA産生細胞 / 好酸球
Outline of Final Research Achievements

CCL28 is expressed in the mucosal tissues and to attract IgA-antibody secreting cells (IgA-ASCs) via CCR10. CCL28 has an antimicrobial activity against microbes in vitro. However, in vivo evidence for the role of CCL28 in the mucosal immunity remains scanty. We generated CCL28-deficient mice and demonstrated that CCL28-deficient mice showed reduced numbers and altered distribution of IgA-ASCs in the colon. The IgA contents in the fecal extracts were low. The average amounts of IgA secreted by a single IgA-ASC isolated from the lamina propria of the colon was reduced. Furthermore, the 16S rRNA sequencing analysis of feces revealed an increase of the Class Bacilli. Consistent with the low IgA production and altered microbiota in the colon, CCL28-deficient mice had aggravated colitis upon treatment with dextran sulfate, which was ameliorated by oral antibiotics. Therefore, CCL28 has an role in the mucosal immunity of the colon as a chemoattractant with a direct antimicrobial activity.

Report

(4 results)
  • 2016 Annual Research Report   Final Research Report ( PDF )
  • 2015 Research-status Report
  • 2014 Research-status Report
  • Research Products

    (9 results)

All 2017 2016 2015 2014

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 2 results,  Acknowledgement Compliant: 2 results) Presentation (4 results)

  • [Journal Article] IL-35 suppresses lipopolysaccharide-induced airway eosinophilia in EBI3-deficient mice.2017

    • Author(s)
      Kanai K, Park AM, Yoshida H, Tsunoda I, Yoshie O.
    • Journal Title

      Journal of Immunology

      Volume: 198(1) Issue: 1 Pages: 119-127

    • DOI

      10.4049/jimmunol.1600506

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Upregulated CCL28 expression in the nasal mucosa in experimental allergic rhinitis: Implication for CD4(+) memory T cell recruitment2016

    • Author(s)
      Nagakubo D, Yoshie O, Hirata T
    • Journal Title

      Cellular Immunology

      Volume: 302 Pages: 58-62

    • DOI

      10.1016/j.cellimm.2016.02.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] CCR4 and its ligands: from bench to bedside2015

    • Author(s)
      Yoshie O, Matsushima K
    • Journal Title

      International Immunology

      Volume: 27 Issue: 1 Pages: 11-20

    • DOI

      10.1093/intimm/dxu079

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional roles of evolutionary conserved motifs and residues in vertebrate chemokine receptors2015

    • Author(s)
      Nomiyama H, Yoshie O
    • Journal Title

      Journal of Leukocyte Biology

      Volume: 97 Issue: 1 Pages: 39-47

    • DOI

      10.1189/jlb.2ru0614-290r

    • Related Report
      2014 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of colonic IgA-secreting cells in the cecal patch2014

    • Author(s)
      Masahata, K., Umemoto, E., Kayama, H., Kotani, M., Nakamura, S., Kurakawa, T., Kikuta, J., Gotoh, K., Motooka, D., Sato, S., Higuchi, T., Baba, Y., Kurosaki, T., Kinoshita, M., Shimada, Y., Kimura, T., Okumura, R., Takeda, A., Tajima, M., Yoshie, O., Fukuzawa, M., Kiyono, H., Fagarasan, S., Iida, T., Ishii, M., Takeda, K
    • Journal Title

      Nature Comm

      Volume: 5 Issue: 1 Pages: 3704-3704

    • DOI

      10.1038/ncomms4704

    • Related Report
      2014 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ケモカインCCL28の欠損はDSS腸炎モデルマウスにおける病態を増悪させる2017

    • Author(s)
      神原弘和、松尾一彦、山本真也、長久保大輔、義江 修、中山隆志
    • Organizer
      日本薬学会第137年会
    • Place of Presentation
      仙台国際センター (宮城県仙台市)
    • Year and Date
      2017-03-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] CCL28 plays an intestinal protection against microbes in vivo as a chemoattractant for IgA-secreting cells and also as an antimicrobial activity.2015

    • Author(s)
      Matsuo K, Fujita M, Nakayaa T, Yoshie O.
    • Organizer
      第 44 回日本免疫学会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2015-11-18
    • Related Report
      2015 Research-status Report
  • [Presentation] ケモカイン MEC/CCL28 の腸管組織における生物学的役割2015

    • Author(s)
      松尾一彦、 北田卓也、重田暁子、藤田貢、義江修、中山隆志
    • Organizer
      日本薬学会第 135 年会
    • Place of Presentation
      神戸学院大学 (神戸市)
    • Year and Date
      2015-03-26 – 2015-03-28
    • Related Report
      2014 Research-status Report
  • [Presentation] ケモカイン MEC/CCL28 は IgA 抗体産生細胞の腸管組織への遊走を制御することで DSS 誘発性大腸炎の劇症化抑制に寄与する2014

    • Author(s)
      北田卓也、松尾一彦、重田 暁子、藤田貢、義江修、中山隆志
    • Organizer
      生体機能と創薬シンポジウム 2014
    • Place of Presentation
      近畿大学 (東大阪市)
    • Year and Date
      2014-08-28 – 2014-08-29
    • Related Report
      2014 Research-status Report

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Published: 2014-04-04   Modified: 2020-01-20  

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