Development of the diagnostic method for detection of multidrug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii using efflux pumps as a target, and its application to infection control
Project/Area Number |
26460657
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
|
Research Institution | Tokai University |
Principal Investigator |
ASAI Satomi 東海大学, 医学部, 准教授 (60365989)
|
Co-Investigator(Renkei-kenkyūsha) |
MIYACHI Hayato 東海大学, 医学部, 教授 (20174196)
|
Research Collaborator |
YOSHIHARA Eisaku 東海大学, 工学部, 客員准教授
OHSHIMA Toshio 東海大学, 医学部付属病院, 臨床検査技師
|
Project Period (FY) |
2014-04-01 – 2017-03-31
|
Project Status |
Completed (Fiscal Year 2016)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2016: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2015: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2014: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | 緑膿菌 / アシネトバクター / 多剤耐性菌 / 薬剤排出ポンプ / Bam / 簡易迅速検査 / 感染制御 / real time RT-PCR / 簡易検査 / 排出ポンプ / 多剤耐性 / 薬剤耐性 / 耐性獲得機構 |
Outline of Final Research Achievements |
The mutations in the genes responsible for the development of antimicrobial resistance were investigated in the drug-resistant Pseudomonas aeruginosa and Acinetobacter baumannii which showed resistance to at least 2 among imipenem, amikacin and ciprofloxacin. As a result, the contribution of the efflux pumps was found involved in almost all drug-resistant strains. On the other hand, the complex protein which supplied s peptides for efflux pumps did not significantly increased. The monitoring of the efflux pumps of P. aeruginosa and Acinetobacter baumannii was suggested to be useful in infection control.
|
Report
(4 results)
Research Products
(90 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Preoperative Spirometric Screening And Pharmacotherapy In The Patients With Obstructive Pulmonary Dysfunction.2014
Author(s)
Takiguchi H, Niimi K, Tomomatsu H, Tomomatsu K, Hayama N, Oguma T, Aoki T, Urano T, Asai S, Miyachi H,Abe T, Asano K
Organizer
American Thoracic Society 2014 International Conference
Place of Presentation
San Diego
Year and Date
2014-05-16 – 2014-05-21
Related Report
-
-
-