Phosphorylation of ATBF1 and its intracellular localization in malignant mestothelioma - investigation of the association with malignant behavior

• Project/Area Number: 26460660
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Kanazawa Medical University
• Principal Investigator: MINATO Hiroshi 金沢医科大学, 医学部, 教授 (10293367)
• Co-Investigator(Kenkyū-buntansha): 竹上 勉 金沢医科大学, 総合医学研究所, 教授 (10113490) ; 中田 聡子 金沢医科大学, 医学部, 助教 (30569091) ; 三浦 裕 名古屋市立大学, 大学院医学研究科, 准教授 (90285198)
• Project Period (FY): 2014-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 悪性中皮腫 / ATBF1 / 癌抑制遺伝子 / 培養細胞 / 細胞内局在 / 核細胞質移行 / 遺伝子ノックアウト / リン酸化 / 脱リン酸化

Outline of Final Research Achievements

We cultured human malignant mesothelioma cell lines (NCI-H 2452, MSTO-211 H, NCI-H 28) and benign mesothelial cell line (Met-5 A) to explore the role of ATBF 1 in malignant mesothelioma. ATBF1 knockout strain was prepared from Met-5A strain using a CRISPR method. Luc gene was inserted into these Met-5A sub-strains and MSTO-211 H, NCI-H 28 strain and transplanted into the abdominal cavity of nude mice. By observing the change in the amount of luminescence, tumor growth was successfully confirmed intraabdominal cavity in MSTO-211H, whereas tumor growth could not be confirmed in Met-5A, NCI-H28 strain after about 2 weeks. Currently, knockout of ATBF1 alone may not cause tumorigenesis.

Research Products

• [Journal Article] A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression. (2016)
  • Author(s): Kawaguchi M, Hara N, Bilim V, Koike H, Suzuki M, Kim TS, Gao N, Dong Y, Zhang S, Fujinawa Y, Yamamoto O, Ito H, Tomita Y, Naruse Y, Sakamaki A, Ishii Y, Tsuneyama K, Inoue M, Itoh J, Yasuda M, Sakata N, Jung CG, Kanazawa S, Akatsu H, Minato H, Nojima T, Asai K, Miura Y.
  • Journal Title: BMC Cancer. Volume: 16 Issue: 1 Pages: 805-805
  • DOI: 10.1186/s12885-016-2845-5
  • NAID: 120006814095
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Regulation of Ripply1 expression in MDCK organoids. (2015)
  • Author(s): Yoshizaki H, Kuwajima Y, Minato H, Kiyokawa E.
  • Journal Title: Biochem Biophys Res Commun Volume: 468 Issue: 1-2 Pages: 337-342
  • DOI: 10.1016/j.bbrc.2015.10.099
  • Peer Reviewed / Open Access
• [Journal Article] NAB2-STAT6 fusion gene analysis in two cases of meningeal solitary fibrous tumor/hemangiopericytoma with late distant metastases. (2015)
  • Author(s): Nakada S, Minato H, Takegami T, Kurose N, Ikeda H, Kobayashi M, Sasagawa Y, Akai T, Kato T, Yamamoto N, Nojima T.
  • Journal Title: Brain Tumor Pathol Volume: 32 Issue: 4 Pages: 268-274
  • DOI: 10.1007/s10014-015-0220-x
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Thymic lymphoid hyperplasia with multilocular thymic cysts diagnosed before the Sjögren syndrome diagnosis. (2015)
  • Author(s): Minato H, Kinoshita E, Nakada S, Nojima T, Tanaka M, Usuda K, Sagawa M, Iwao H, Tanaka M, Doai M, Takahashi T, Shibata N.
  • Journal Title: Diagn Pathol Volume: 10 Issue: 1 Pages: 103-103
  • DOI: 10.1186/s13000-015-0332-y
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Comparative immunohistochemical analysis of IMP3, GLUT1, EMA, CD146, and desmin for distinguishing malignant mesothelioma from reactive mesothelial cells. (2014)
  • Author(s): Minato H, Sagawa M, Sakuma T, et al.
  • Journal Title: Am J Clin Pathol Volume: 141 Issue: 1 Pages: 85-93
  • DOI: 10.1309/ajcp5knl7qtellyi
  • Peer Reviewed
• [Journal Article] Narrow band imaging-guided endoscopic biopsy for intraventricular and paraventricular brain tumors: clinical experience with 14 cases. (2014)
  • Author(s): Sasagawa Y, Akai T, Nakada S, Minato H, Tachibana O, Nojima T, Iizuka H.
  • Journal Title: Acta Neurochir (Wien). Volume: 156 Issue: 4 Pages: 681-687
  • DOI: 10.1007/s00701-014-1995-y
  • Peer Reviewed
• [Journal Article] A rare Japanese case with a NUT midline carcinoma in the nasal cavity : A case report with immunohistochemical and genetic analyses (2014)
  • Author(s): Suzuki S, Sugimura H et al.
  • Journal Title: Pathol Res Pract Volume: 210(6) Issue: 6 Pages: 383-8
  • DOI: 10.1016/j.prp.2014.01.013
  • Peer Reviewed
• [Journal Article] Activated K-RAS and its effect on morphological appearance (2014)
  • Author(s): Etsuko Kiyokawa, Hiroshi Minato
  • Journal Title: J Biochem Volume: 156 Issue: 3 Pages: 137-145
  • DOI: 10.1093/jb/mvu041
  • NAID: 40020192539
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Rheumatoid nodule in the lower lip of a patient with rheumatoid arthritis: a novel case report and review of literature. (2014)
  • Author(s): Kato K, Segami N, Fukuda H, Minato H.
  • Journal Title: J Oral Maxillofac Surg. Volume: 72 Issue: 8 Pages: 1532.e1-1532.e5
  • DOI: 10.1016/j.joms.2014.03.034
  • Peer Reviewed
• [Presentation] 悪性中皮腫とアデノマトイド腫瘍におけるEZH2及びp16蛋白発現 (2016)
  • Author(s): 湊 宏、熊谷泉那、塩谷晃広、中田聡子、黒瀬望、野島孝之
  • Organizer: 第105回日本病理学会総会
  • Place of Presentation: 仙台国際センター(宮城県仙台市)
  • Year and Date: 2016-05-12
• [Presentation] A tracheal tumor of a young girl. (2015)
  • Author(s): Hiroshi Minato, Akari Iwakoshi, Masaki Hasegawa, Shu Ichihara, Suzuko Moritani, Toyonori Tsuzuki, Yasushi Yatabe
  • Organizer: The 6th Korean-Japanese Conjoint Slide Conference of Pulmonary Pathology
  • Place of Presentation: Seoul (Korea)
  • Year and Date: 2015-10-31
  • Int'l Joint Research
• [Presentation] 悪性中皮腫とアデノマトイド腫瘍におけるEZH2及びp16蛋白発現の相違と、鑑別診断における有用性 (2015)
  • Author(s): 湊 宏、薄田勝男、佐川元保
  • Organizer: 第56回日本肺癌学会学術集会
  • Place of Presentation: パシフィコ横浜(神奈川県横浜市)
  • Year and Date: 2015-10-26
• [Presentation] 14歳女児の気管内に発生したlow-grade sarcoma with EWSR1-CREB1 fusion geneの一例 (2015)
  • Author(s): 岩越朱里、森谷鈴子、都築豊徳、谷田部恭、湊 宏、野島孝之、長谷川正規、市原周
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 名古屋国際会議場(愛知県名古屋市)
  • Year and Date: 2015-04-30
• [Presentation] 転移を来した髄膜原発cellular solitary fibrous tumor 2例におけるNAB2-STAT6融合遺伝子解析 (2015)
  • Author(s): 中田聡子、湊 宏、竹上 勉、黒瀬 望、木下英理子、三輪有香子、塩谷晃広、池田博子、小林雅子、野島孝之
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 同上
  • Year and Date: 2015-04-30
• [Presentation] 鼻副鼻腔Nut midline carcinoma (NMC)の臨床病理学的解析とFISHの応用 (2015)
  • Author(s): 湊 宏、木下英理子、中田聡子、黒瀬 望、野島孝之、田中祐吉、鈴木潮人、谷岡書彦
  • Organizer: 第104回日本病理学会総会
  • Place of Presentation: 同上
  • Year and Date: 2015-04-30
• [Book] 呼吸器 Q16-21. In 読む・解く・学ぶ 細胞診 Quiz 50 ベーシック篇 (2014)
  • Author(s): 湊 宏
  • Total Pages: 128
  • Publisher: 診断と治療社
• [Book] 呼吸器 Q17-22. In 読む・解く・学ぶ 細胞診 Quiz 50 アドバンス篇 (2014)
  • Author(s): 湊 宏
  • Total Pages: 128
  • Publisher: 診断と治療社