Phosphorylation of ATBF1 and its intracellular localization in malignant mestothelioma - investigation of the association with malignant behavior
Project/Area Number |
26460660
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Laboratory medicine
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Research Institution | Kanazawa Medical University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
竹上 勉 金沢医科大学, 総合医学研究所, 教授 (10113490)
中田 聡子 金沢医科大学, 医学部, 助教 (30569091)
三浦 裕 名古屋市立大学, 大学院医学研究科, 准教授 (90285198)
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Project Period (FY) |
2014-04-01 – 2017-03-31
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Project Status |
Completed (Fiscal Year 2016)
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Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2014: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 悪性中皮腫 / ATBF1 / 癌抑制遺伝子 / 培養細胞 / 細胞内局在 / 核細胞質移行 / 遺伝子ノックアウト / リン酸化 / 脱リン酸化 |
Outline of Final Research Achievements |
We cultured human malignant mesothelioma cell lines (NCI-H 2452, MSTO-211 H, NCI-H 28) and benign mesothelial cell line (Met-5 A) to explore the role of ATBF 1 in malignant mesothelioma. ATBF1 knockout strain was prepared from Met-5A strain using a CRISPR method. Luc gene was inserted into these Met-5A sub-strains and MSTO-211 H, NCI-H 28 strain and transplanted into the abdominal cavity of nude mice. By observing the change in the amount of luminescence, tumor growth was successfully confirmed intraabdominal cavity in MSTO-211H, whereas tumor growth could not be confirmed in Met-5A, NCI-H28 strain after about 2 weeks. Currently, knockout of ATBF1 alone may not cause tumorigenesis.
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Report
(4 results)
Research Products
(17 results)
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[Journal Article] A diagnostic marker for superficial urothelial bladder carcinoma: lack of nuclear ATBF1 (ZFHX3) by immunohistochemistry suggests malignant progression.2016
Author(s)
Kawaguchi M, Hara N, Bilim V, Koike H, Suzuki M, Kim TS, Gao N, Dong Y, Zhang S, Fujinawa Y, Yamamoto O, Ito H, Tomita Y, Naruse Y, Sakamaki A, Ishii Y, Tsuneyama K, Inoue M, Itoh J, Yasuda M, Sakata N, Jung CG, Kanazawa S, Akatsu H, Minato H, Nojima T, Asai K, Miura Y.
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Journal Title
BMC Cancer.
Volume: 16
Issue: 1
Pages: 805-805
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] NAB2-STAT6 fusion gene analysis in two cases of meningeal solitary fibrous tumor/hemangiopericytoma with late distant metastases.2015
Author(s)
Nakada S, Minato H, Takegami T, Kurose N, Ikeda H, Kobayashi M, Sasagawa Y, Akai T, Kato T, Yamamoto N, Nojima T.
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Journal Title
Brain Tumor Pathol
Volume: 32
Issue: 4
Pages: 268-274
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Thymic lymphoid hyperplasia with multilocular thymic cysts diagnosed before the Sjögren syndrome diagnosis.2015
Author(s)
Minato H, Kinoshita E, Nakada S, Nojima T, Tanaka M, Usuda K, Sagawa M, Iwao H, Tanaka M, Doai M, Takahashi T, Shibata N.
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Journal Title
Diagn Pathol
Volume: 10
Issue: 1
Pages: 103-103
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Presentation] A tracheal tumor of a young girl.2015
Author(s)
Hiroshi Minato, Akari Iwakoshi, Masaki Hasegawa, Shu Ichihara, Suzuko Moritani, Toyonori Tsuzuki, Yasushi Yatabe
Organizer
The 6th Korean-Japanese Conjoint Slide Conference of Pulmonary Pathology
Place of Presentation
Seoul (Korea)
Year and Date
2015-10-31
Related Report
Int'l Joint Research
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