| Project/Area Number |
26460665
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Laboratory medicine
|
|---|
| Research Institution | Gunma University |
|---|
Principal Investigator |
Handa Hiroshi 群馬大学, 医学部附属病院, 講師 (90282409)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
村上 博和 群馬大学, 大学院保健学研究科, 教授 (40166260)
山根 有人 群馬大学, 大学院医学系研究科, 講師 (10420192)
|
|---|
| Project Period (FY) |
2014-04-01 – 2017-03-31
|
| Project Status |
Completed (Fiscal Year 2016)
|
| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2015: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2014: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 多発性骨髄腫 / non coding RNA / 次世代シークエンサー / 髄外形質細胞腫 / ストレス / long non coding RNA / MALAT1 / プロテアソーム阻害 / Long non-coding RNA / 細胞ストレス / long non-coding RNA |
|---|
| Outline of Final Research Achievements |
We analyzed multiple myeloma (MM), precancerous stage MGUS, refractory extramedullary plasmacytoma (EMM) by the next-generation sequencer how non coding RNA was associated with disease progression. As a result, MALAT1 and NEAT1 expressed higher in MM and EMM than in MGUS, particularly, MALAT1 expressed markedly higher in EMM with several thousand fold. We knocked down MALAT1 in MM cell line, but the change was not found in a cell proliferation, cell motility gene expression. MALAT1 and NEAT1 expression increased by bortezomib and doxorubicin. It was suggested that induced MALAT1 and NEAT1 by drug stress were associated with EMM formation.
|
