| Project/Area Number |
26460679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Yamaguchi University |
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Principal Investigator |
OKANO Kozue 山口大学, 医学(系)研究科(研究院), 教授 (50160693)
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| Co-Investigator(Kenkyū-buntansha) |
野島 順三 山口大学, 医学(系)研究科(研究院), 教授 (30448071)
丸田 雄一 山口大学, 医学部, 特別医学研究員 (30543970)
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| Research Collaborator |
SHITAMOTO Kazuki
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| Project Period (FY) |
2014-04-01 – 2017-03-31
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| Project Status |
Completed (Fiscal Year 2016)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2014: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 活性化血小板 / マイクロパーティクル / ELISA法 / 血小板関連抗体 / 抗AnnexinV抗体 / 採血条件 / 不整脈 / 抗凝固薬 / 血小板特異的抗体 / aPLT-MP / 赤血球 / 好中球 / 単球 / 血管内皮細胞 / サンドイッチ法 / ペルオキシダーゼ標識抗マウス・ウサギ抗体 / ドットブロット法・免疫染色法 |
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| Outline of Final Research Achievements |
Activated-Platelet derived microparticle (aPLT-MP) has a strong influence on the blood clot formation and inflammatory, however, measuring method and mechanism of clot formation have not been clarified. We conducted a fundamental examination of an assay by ELISA using various platelet-related antibodies to determine aPLT-MP. Citrated plasma samples were obtained from 13 healthy volunteers, 72 patients with atrial fibrillation, 60 with hypertension, and 41 with diabetes mellitus.
The sensitivity and specificity of aPLT-MP varied according to the combination of platelet-related antibodies, and anti-AnnexinV polyclonal antibody for solid-phase and anti-GPIb monoclonal antibody for detector was the most suitable combination for aPLT-MP. There was no significant difference in PLT-MP values of volunteers between healthy and patients. Influencing factors to aPLT-MP values were arrhythmia and anticoagulant drugs.
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